Detection of staphylococcal enterotoxin B in spiked food samples

Contamination of food with infectious agents, intentional or not, is a global concern with far-reaching economic and social impact. Staphylococcal enterotoxins are a major cause of food poisoning, but most methods for the identification of these agents in food require extensive pretreatment or concentration of the sample prior to analysis. The array biosensor was developed as a portable device for the simultaneous analysis of multiple complex samples for multiple targets with minimal sample preparation. In this study, we use an array biosensor to expand and improve on a staphylococcal enterotoxin B (SEB) assay with the ultimate intent of incorporating testing for SEB into a battery of sensitive and convenient assays for food safety validation. In addition to buffer studies, six different types of food samples, including beverages, homogenates of fruit and meat, and carcass washings, were spiked with SEB, incubated for at least 2 h to permit antigen sequestration, and assayed. For all samples, there were differences in fluorescence intensity, but 0.5 ng of SEB per ml could be detected in <20 min with little if any pretreatment and no sample preconcentration.     

<Emphasis Type="Italic">k</Emphasis>-Nearest-Neighbor Clustering and Percolation Theory

Let P be a realization of a homogeneous Poisson point process in ℝ ^d with density 1. We prove that there exists a constant k _d , 1<k _d <∞, such that the k-nearest neighborhood graph of P has an infinite connected component with probability 1 when k≥k _d . In particular, we prove that k ₂≤213. Our analysis establishes and exploits a close connection between the k-nearest neighborhood graphs of a Poisson point set and classical percolation theory. We give simulation results which suggest k ₂=3. We also obtain similar results for finite random point sets. Part of the work was done while S.-H. Teng was at Xerox Palo Alto Research Center and MIT. The work of F.F. Yao was supported in part by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China [Project No. CityU 1165/04E].     

Learning to use new technologies by older adults: Perceived difficulties,experimentation behaviour and usability

This paper examines the factors and theoretical frameworks for the adoption of technology for older adults, and proposes two models of technology acceptance and rejection, one from an ease of learning perspective, and one from a system and user perspective. Both models are supported from reports on two case studies of older adults using handheld touchscreen tablet devices; one in which the participants are supported during tasks primarily related to walking and navigation, and one in which participants are unsupported in communication related tasks. The first study shows the powerful role that facilitating conditions have for learning how to use digital technologies for this user group, whether supporting through step by step guidance, providing a friendly space to use trial and error methods, and/or provision of a manual. The second study shows the pitfalls of a lack of facilitating conditions during initial use, and highlights potential for appropriate design in helping to avoid some user errors during this phase.     

A developmental perspective on the study of depression: Children's evaluative reactions to sadness.

Examined developmental differences in emotional expression. It is suggested that there are cognitive and character structures that may be prerequisites for certain adult reactions to painful life events. The self-denigrating adult may be a discrepancy between self-representation and ideal self. In a child, these structures may not yet be stable enough to withstand such critical self-appraisal. 35 5- and 7-yr-olds were asked to give liking ratings to hypothetical children who had undergone a painful life event and to give evaluative ratings of themselves in the same situations. Older Ss evaluated a hypothetical other and themselves negatively, whereas the 5-yr-olds could not extend their negative evaluation of a sad other to themselves. Results are discussed in terms of developmental changes in the depressive reaction. (31 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The role of market and technical downsizing in reducing carbon emissions from the Swedish new car fleet

Doubts have been raised on whether the car industry will manage to reach the goal set by the Voluntary Agreement with the European Commission, unless tougher measures are taken to reduce CO₂ emissions. Taking a stance from the concept of downsizing, we study the historical development of two strategies: first, shifting the market toward smaller cars, market downsizing; second, a technical downsizing, i.e., technical improvements that have enabled a reduction of engine size. We focus on the Swedish new car market from 1975 to 2002. Analysis is done by combining sales statistics with databases over car model parameters. The development of the top and bottom 10% and 20% of the car market of various parameters points to an increased differentiation of the market; still, the market share of small models remains low. We identify the potential for downsizing created by increase of maximum specific torque and power and conclude that these have enabled the cylinder volume to stabilize around 2 l. Increase in engine size has also partly been dampened by the utilization of supercharging. The potential savings of fuel use presented by the introduction of a sixth gear in manual gearboxes have not materialized since gearshifts have not systematically been used to lower engine speed. We conclude that there are few signs of a downsizing in the Swedish new car fleet. From a market perspective, larger cars are still dominant and the technical potentials to reduce engine size have not been harnessed.

A peculiar form of amicrobial pustulosis of the folds associated with systemic lupus erythematosus and other auto-immune diseases

This article continues the series of electronic microscopical investigations of tick attachment and host's inflammatory reaction in the attachment site. In all this works we used larvae of different Ixodid ticks but the same species of the host (white mouse), Ixodid species having different type of attachment. Dermacentor marginatus (Amosova, 1989a) is characterized by the superficial penetration of mouth parts, the abundance of cement around them and on the surface of the host's skin, the mouth parts of Hyalomma asiaticum are fully inserted and the superficial cement is minimal (Amosova, 1989b) and Ixodes ricinus has mouth parts inserted more deeply in the skin tissue of the host and the cement substance localised only on the surface of the skin at the 1st and 2nd days of feeding period. For this investigation we choose Haemaphysalis longicornis because it is known (see Kemp et al., 1982) that genus Haemaphysalis has the most superficial localisation of the mouth parts in the attachment site. We have studied a fine structure of cement cone and the cement substance surrounding the mouth parts in the skin and have demonstrated that it is very similar to the Dermacentor marginatus cement structure. The pattern of cellular response is similar to D. marginatus lesion too.     

Trauma, dissociation, and conflict: The space where neuroscience, cognitive science, and psychoanalysis overlap.

The authors describe their scientific and clinical interests in developing the panel Trauma, Dissociation, and Conflict: The Space Where Neuroscience, Cognitive Science, and Psychoanalysis Overlap, given at the 22nd Annual Spring Meeting of the Division of Psychoanalysis of the American Psychological Association. They cite the influence of the panelists, Philip M. Bromberg, Wilma Bucci, and Joseph LeDoux, on their own work. Specializing in the treatment of chronic pain, Anderson has developed a relational intersubjective technique informed by this interdisciplinary approach. Gold's work has focused on the distinction between dissociative processes and repression in traumatic memory. The relevance of trauma, dissociation, and conflict, heightened since September 11, 2001, could not have been anticipated in the planning of the panel. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Making Large‐Area Titanium Disulfide Films at Reduced Temperature by Balancing the Kinetics of Sulfurization and Roughening

The synthesis of large‐area TiS₂ thin films is reported at temperatures as low as 500 °C using a scalable two‐step method of metal film deposition followed by sulfurization in an H₂S gas furnace. It is demonstrated that the lowest‐achievable sulfurization temperature depends strongly on the oxygen background during sulfurization. This dependence arises because Ti? O bonds present a substantial kinetic and thermodynamic barrier to TiS₂ formation. Lowering the sulfurization temperature is important to make smooth films, and to enable integration of TiS₂ and related transition metal dichalcogenides—including metastable phases and alloys—into device technology.     

Protein design for non-aqueous solvents

Improving protein stability in unnatural and suboptimal environmentsis a promising application of protein engineering technology.Carefully designed amino acid alterations may lead to dramaticpositive effects on the stability of proteins under highly perturbingconditions, such as in non-aqueous solvents. Applications ofbiocatalysts and proteins with specific binding capabilitiesin the chemical industry have been severely limited by constraintsplaced on the solvent environment. With the advent of convenientmethods for altering the amino acid composition and even synthesizingentirely new protein molecules, it is worthwhile to considerengineering proteins for stability in non-aqueous solvents.In order to identify the features that a protein would needfor stability in organic media, we have been studying the structureand properties of the hydrophobic protein crambin. Crambin isunique in that it is soluble and stable in very high concentrationsof polar organic solvents. Crambin and its water-soluble homologsoffer a powerful demonstration of protein engineering for non-aqueoussolvents. This paper describes the structural features thatcontribute to crambin's special properties. Based on these observationsand consideration of how non-aqueous solvents affect the interactionsimportant in protein folding, a set of rules for designing non-aqueoussolvent-stable proteins is proposed.