A Dual Selection Module for Directed Evolution of Genetic Circuits

We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.     

Collaboration and Teamwork: Immersion and Presence in an Online Learning Environment

In the world of OTIS, an online Internet School for occupational therapists, students from four European countries were encouraged to work collaboratively through problem-based learning by interacting with each other in a virtual semi-immersive environment. This paper describes, often in their own words, the experience of European occupational therapy students working together across national and cultural boundaries. Collaboration and teamwork were facilitated exclusively through an online environment, since the students never met each other physically during the OTIS pilot course. The aim of the paper is to explore the observations that (1) there was little interaction between students from different tutorial groups and (2) virtual teamwork developed in each of the cross-cultural tutorial groups. Synchronous data from the students was captured during tutorial sessions and peer-booked meetings and analyzed using the qualitative constructs of immersion, presence and reflection in learning. The findings indicate that immersion was experienced only to a certain extent. However, students found both presence and shared presence, within their tutorial groups, to help collaboration and teamwork. Other evidence suggests that communities of interest were established. Further study is proposed to support group work in an online learning environment. It is possible to conclude that collaborative systems can be designed, which encourage students to build trust and teamwork in a cross cultural online learning environment.This revised version was published online in March 2005 with corrections to the cover dateFunded by the European Union through the TENTelecom programme.     

Identification of some marine oil constituents by chromatography

Summary and Conclusion A scheme for the separation and identification of some constituents of marine oils was developed. A preliminary separation on a silicic acid column with five solvent systems was followed by further separation and identification on silicic acid impregnated glass-fiber filter paper. This method can be used successfully for qualitative determinations, but the irregularities in the density of the glass paper prevent an accurate quantitative assay. Supported, in part, by grants from the Fish and Wildlife Service of the United States Department of the Interior, the Robert A. Welch Foundation, and the Office of Naval Research.     

Thermodynamically calculated phase diagrams for the Co-Cr-Ta and Co-Cr-Nb systems

Phase diagrams for the Co-Cr-Ta and Co-Cr-Nb systems have been calculated from thermodynamic data for the binary systems involved. Eleven isothermal sections for temperatures between 2200 and 1500 K are given. The calculations are in good agreement with the limited available experimental phase diagram data for these systems.     

A developmental perspective on the study of depression: Children's evaluative reactions to sadness.

Examined developmental differences in emotional expression. It is suggested that there are cognitive and character structures that may be prerequisites for certain adult reactions to painful life events. The self-denigrating adult may be a discrepancy between self-representation and ideal self. In a child, these structures may not yet be stable enough to withstand such critical self-appraisal. 35 5- and 7-yr-olds were asked to give liking ratings to hypothetical children who had undergone a painful life event and to give evaluative ratings of themselves in the same situations. Older Ss evaluated a hypothetical other and themselves negatively, whereas the 5-yr-olds could not extend their negative evaluation of a sad other to themselves. Results are discussed in terms of developmental changes in the depressive reaction. (31 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Further Characterization of Intrastriatal Lipopolysaccharide Model of Parkinson’s Disease in C57BL/6 Mice

Parkinson’s disease (PD) is the most common movement disorder, characterized by progressive degeneration of the nigrostriatal pathway, which consists of dopaminergic cell bodies in substantia nigra and their neuronal projections to the striatum. Moreover, PD is associated with an array of non-motor symptoms such as olfactory dysfunction, gastrointestinal dysfunction, impaired regulation of the sleep-wake cycle, anxiety, depression, and cognitive impairment. Inflammation and concomitant oxidative stress are crucial in the pathogenesis of PD. Thus, this study aimed to model PD via intrastriatal injection of the inflammagen lipopolysaccharide (LPS)to investigate if the lesion causes olfactory and motor impairments, inflammation, oxidative stress, and alteration in synaptic proteins in the olfactory bulb, striatum, and colon. Ten µg of LPS was injected unilaterally into the striatum of 27 male C57BL/6 mice, and behavioural assessment was conducted at 4 and 8 weeks post-treatment, followed by tissue collection. Intrastriatal LPS induced motor impairment in C57BL/6 mice at 8 weeks post-treatment evidenced by reduced latency time in the rotarod test. LPS also induced inflammation in the striatum characterized by increased expression of microglial marker Iba-1 and astrocytic marker GFAP, with degeneration of dopaminergic neuronal fibres (reduced tyrosine hydroxylase immunoreactivity), and reduction of synaptic proteins and DJ-1 protein. Additionally, intrastriatal LPS induced inflammation, oxidative stress and alterations in synaptic proteins within the olfactory bulb, although this did not induce a significant impairment in olfactory function. Intrastriatal LPS induced mild inflammatory changes in the distal colon, accompanied by increased protein expression of 3-nitrotyrosine-modified proteins. This model recapitulated the major features of PD such as motor impairment and degeneration of dopaminergic neuronal fibres in the striatum, as well as some pathological changes in the olfactory bulb and colon; thus, this model could be suitable for understanding clinical PD and testing neuroprotective strategies.     

Cellular Disulfide Bond Formation in Bioactive Peptides and Proteins

Bioactive peptides play important roles in metabolic regulation and modulation and many are used as therapeutics. These peptides often possess disulfide bonds, which are important for their structure, function and stability. A systematic network of enzymes—a disulfide bond generating enzyme, a disulfide bond donor enzyme and a redox cofactor—that function inside the cell dictates the formation and maintenance of disulfide bonds. The main pathways that catalyze disulfide bond formation in peptides and proteins in prokaryotes and eukaryotes are remarkably similar and share several mechanistic features. This review summarizes the formation of disulfide bonds in peptides and proteins by cellular and recombinant machinery.     

Evaluating the use of digital image correlation for strain measurement in historic tapestries using representative deformation fields

An analysis technique to assess the viability of digital image correlation (DIC) in tracking the full‐field strains across the surface of hanging historic tapestries is presented. Measurement uncertainty related to the use of the inherent tapestry image in tracking displacements is investigated through use of “synthetic” deformation fields. The latter are generated by mapping the details of a given tapestry image into finite element analyses. The combination of self‐weight loading, material non‐linearity, and image specific heterogeneity (related to slit stitching, damage, and patch‐restorations) serve to generate a bespoke deformation field complex enough to assess the reliability of DIC measurements. Accuracy is evaluated by comparing measured results with the original known deformations. The technique demonstrates that the optimum imaging settings and the choice of subset size for DIC analysis are strongly influenced by the tapestry image and the goal of the measurement, they are found using a compromise between conflicting objectives: minimising measurement error while maximising resolution.