Studies of substrate specificities of lipases from different sources

Although lipases are widely applied in a wide variety of reactions, available information on the factors that are responsible for the substrate specificities of lipases from different sources is scarce. In this paper, nine lipase‐producing microorganism strains were isolated from oil‐containing samples. The properties of these enzymes, including pH optima, temperature optima, thermal stability, and pH stability, vary significantly with the different sources from which these lipases were isolated. The substrate specificities of the nine lipases, including fatty acid and positional specificities, were also studied. The fatty acid specificities of the nine lipases were observably different toward 15 substrates with different carbon chain lengths, different saturation degrees and different side chains. The lipases from S₃ Penicillium citrinum (PCL), MJ₁ Aspergillus niger (ANL), MJ₂ Aspergillus oryzae (AOL), YM Bacillus coughing (BCL), S₉ Geotrichum candidum (GCL), and S₁₁ Candida lypolytica (CLL) showed the strongest specificities to short‐chain esters, and the other lipases showed strong selectivity for medium‐ or long‐chain and branched esters. The positional specificities were examined by analyzing the hydrolytic products of triolein catalyzed by the nine lipases, using TLC. The lipases can be mainly classified into two groups by their specificities for the ester bond at position 2 of triglycerides; one group can selectively hydrolyze the ester bond at position 2 of the triglycerides, the other group cannot. All these nine lipases were divided into four groups by hierarchical clustering analysis on the basis of the results of fatty acid and positional specificities.     

Porosity distribution in highly compressible cake: Experimental and theoretical verification of the dense skin

The rate of filtration and the water content of cake are influenced by the existence of a dense skin in a highly compressible cake. The phenomenon of the dense skin has been rarely studied, and its existence has not been verified experimentally. In this study, the porosity variation in a very compressible cake is measured by using a new experimental apparatus, and with this the existence of dense skin has been established experimentally. ‘Unified theory on solid-liquid separation’, a recently developed theory, is utilized for calculating the porosity variation in a very compressible cake.     

Tri-amelia and phocomelia with multiple malformations resembling Roberts syndrome in a fetus: is it a variant or a new syndrome?

An initial Bentall operation was performed on a patient aged 38 years with annuloaortic ectasia and aortic regurgitation. An echocardiogram and RI angiogram revealed a perigraft aortic aneurysm on the same patient aged 45 years. The aneurysm gradually developed and the maximum diameter reached 90 mm in size. The Redo-Bentall operation (Piehler modification) was successfully done once again on the same patient aged 56 years. The anastmosis between the right coronary orifice and the graft was detached on all sides. The anastmosis between the left coronary orifice and the graft was detached two thirds of all sides.     

隧洞开挖网络进度的优化研究

本文以单项工程为研究对象,以隧洞工程为例,研究了单项工程施工所需用的时间。文中以施工单位所具有的资源,结合施工工作环境、作业空间为约束条件,以费用最小为目标函数,建立了优化模型。由资源的工作性能指标,利用模糊数学原理,对资源间的匹配程度作了定量分析,并进一步推出了不同匹配程度的资源对作业时间的影响的计算式。并从影响各工序完工时间的因素的随机性出发,利用数理统计理论,由各工序的细分活动的规律推出了各工序的完工需用时间的概率表达式,解决了工期与置信度间的关系。最后结合工程实例,求出了一定置信度下,不同资源供应时的最优工期。     

氧化铝中微量锌的测定

在Tween -80存在下 ,于 pH 6.5 -7.5的NaOH -KH2 PO4 缓冲体系中 ,HQANB与锌形成稳定的红色配合物 ,最大吸收峰在 5 5 0nm处 ,摩尔吸光系数ε=1.1× 10 5。锌在 0～ 10ug/ 2 5ml范围内符合比尔定律 .方法选择性好 ,灵敏度高 ,操作简便 ,用于氧化铝中微量锌的测定 ,结果令人满意     

浸胶工艺对Nomex蜂窝性能的影响

本文研究了浸胶工艺对Ｎｏｍｅｘ蜂窝性能的影响，分析探讨了提高Ｎｏｍｅｘ蜂窝性能及外观质量的途径。研究结果表明：采取新的浸胶工艺制作的Ｎｏｍｅｘ蜂窝，各项性能全面达到法国宇航材料标准。     

浅谈钢铁厂煤气管道的规划与设计

本文主要阐述了钢铁厂煤气的种类及规划与设计中应注意的问题,提出了正确的规划与设计方法。     

朴素的认识

2月20日，“中国国际建筑艺术实践展”项目开工典礼在南京珍珠泉佛手湖工地现场举行。此时距这个项目正式启动已经过了一年半了。从主办方提供的资料看．参展建筑师的设计方案可谓各具特色，但也许正是这种有意为之的特色使得建筑真的成了一件展品。如果说迪斯尼乐园里那些城堡和尖塔是孩子们的大玩具的话，那么佛手湖畔这将要建成的24栋建筑就是成人们的大玩具。     

The dynamic effects and compensation of load transitions in robotic systems

The problem of a robot handling a payload typically involves pre-load, load, and post-load stages, and payload transitions between these stages. Although considerable work has focused on the control of robots carrying payloads, few consider the problem of load transitions—one of the most important effects on system performance. This article studies these transitions and presents a compensation method. It is shown that load transitions lead to abrupt (jump) parameters and uncertain transition times in the robot/payload model. A control strategy is proposed that takes the load transitions into account and guarantees the stable path tracking of robots in the face of these transitions. The effectiveness of the proposed control strategy is verified via simulation. © 1994 John Wiley & Sons, Inc.