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101.
IS Han N Ramamurthy JH Yun U Schaller ME Meyerhoff VC Yang 《Canadian Metallurgical Quarterly》1996,10(14):1621-1626
A novel method to monitor specific peptidase activities in biological samples as complex as undiluted plasma/blood is described. The approach is based on the design of synthetic polypeptide substrates in which di- or triarginine sequences are linked to each other via one or more other amino acids recognized specifically by the peptidase to be determined. Detection of chymotrypsin and renin activities using synthetic substrates P4 (F-R-R-R-F-V-R-R-F-NH2) and P5 (R-R-R-L-L-R-R-L-L-R-R-R), respectively, serves to demonstrate the principles of this new assay system. A polyion-sensitive membrane electrode, prepared by doping polymer films with dinonylnaphthalene-sulfonate (DNNS), is shown to exhibit significant nonequilibrium electromotive force (EMF) responses toward these and other polycationic substrates at microgram/milliliter levels under physiological conditions. The same electrode, however, exhibits much smaller total EMF response toward the shorter fragments of the synthetic peptides generated by peptidase activity; hence, the addition of peptidase to a solution containing the synthetic substrate yields a change in electrode EMF response, the rate of which is proportional to the activity of peptidase present. Other synthetic polycationic peptides as well as natural polycationic peptides (e.g., protamine) that lack specific cleavage sites for chymotrypsin and renin, yet are detected by the DNNS-based membrane electrode, do not elicit any significant change in EMF response in the presence of the peptidases, confirming the feasibility and utility of the proposed bioanalytical method. 相似文献
102.
Calculation of Mass Attenuation Coefficients of Beta Particles 总被引:1,自引:0,他引:1
Yi C.Y.; Han H.S.; Cho W.K.; Park U.J.; Jun J.S.; Chai H.S. 《Radiation protection dosimetry》1998,78(3):221-229
103.
光催化氧化后四环素的分解率与COD去除率之间关系的探讨 总被引:1,自引:0,他引:1
以粉状无机半导体(ZnO)为催化剂,探讨了水溶液中四环素经光催化氧化分解率和COD去除率之间的关系。实验表明,在本实验中四环素的分解率和COD去除率之间有较好的正相关性。当反庆系统中有足量的活性催化剂和足够的光照时间时,绝大部分四环素将被较彻底地氧化分解成CO2,这不仅有助于光催化反应机理的研究,助于今后在环保中的实际应用。 相似文献
104.
螺丝湾水电站作为云南省电力局( 集团公司) 、迪庆州、省投资公司三方合资建设的电站,是迪庆藏族自治州自建国以来投资最大的一个建设项目,它的建成,开创了滇西北水电资源多方合资开发的先例,有其特殊的经济和政治意义。本文结合螺丝湾水电站建设的实际情况,论述了在市场经济条件下做好螺丝湾电站建设中“三控制”的几项措施 相似文献
105.
G Isenberg S Han A Schiefer MF Wendt-Gallitelli 《Canadian Metallurgical Quarterly》1993,27(10):1800-1809
OBJECTIVE: The aim was to examine whether mitochondrial Ca2+ fluxes are high enough to change mitochondrial and cytosolic calcium concentration during the contraction cycle. METHODS: Isolated guinea pig ventricular myocytes were stimulated with paired voltage clamp pulses until contractions were maximal (2 mM [Ca2+]o, 36 degrees C). At defined times of diastole or systole, the cells were shock frozen. Electron-probe microanalysis measured the concentration of total calcium in mitochondria (sigma Ca(mito)) and surrounding cytosol (sigma Cac). Other experiments were performed to evaluate DNP sensitive mitochondrial Ca2+ uptake from depolarisation induced [Ca2+]c transients (K5indo-1 fluorescence). RESULTS: At end of diastole, sigma Ca(mito) was 446 mumol.litre-1. During systole, sigma Ca(mito) increased with a 20 ms delay. A peak sigma Ca(mito) of 1050 mumol.litre-1 was measured 40 ms after start of systole, while 95 ms after start of systole sigma Ca(mito) had fallen to 530 mumol.litre-1. From the changes in sigma Ca(mito) the rates of net mitochondrial Ca2+ flux were estimated at 100 nmol.s-1 x mg-1 protein for Ca2+ influx and 36 nmol.s-1 x mg-1 protein for Ca2+ egress. Decay of sigma Ca(mito) was coupled to a rise in sigma Na(mito). sigma Cl(mito) and sigma K(mito) rose and fell in parallel with sigma Ca(mito), suggesting Ca2+ activation of mitochondrial anion and cation channels. Activation of the non-specific permeability can be excluded. Block of mitochondrial Ca2+ uptake with DNP (100 microM) or FCCP (10 microM) increased the amplitude of the [Ca2+]c transients for 1-3 min by about 50%; evaluation of mitochondrial Ca2+ uptake from DNP sensitive difference signals, however, was hampered by sequestration of mitochondrial Ca2+ into the sarcoplasmic reticulum. CONCLUSIONS: Mitochondrial calcium content changes during each individual contraction cycle; a substantial amount of calcium is taken up during the systole and released during later systole and diastole. 相似文献
106.
107.
Gang Bao 《Wireless Networks》1996,2(3):229-237
Due to the high frame error rate in wireless communication channels, an additional link layer protocol, Radio Link Protocol (RLP), has been introduced in the newly approvedData Services Option Standard for Wideband Spread Spectrum Digital Cellular System. In this paper, we investigate performance issues of a typical Cod Division Multiple Access (CDMA) wireless link using the protocol stack given in the standard. In particular, we focus on the dynamics of the TCP and RLP layers of the protocol stack since the fluctuation of system performance is largely caused by Automatic Repeat Request (ARQ) mechanisms implemented at these two layers. We compare the network performance of default parameter setting to those of other possible parameter settings. Analytical and simulation results presented in this paper can provide guidance to those attempting to further improve performance of interest. 相似文献
108.
本文介绍了采金船渡汛时的停泊方向及采金船在洪水中可能产生拖曳力的估算方法,为了防止采金船汛期发生溜船事故,建议采用缆绳牵拉固船的措施,以保障采金船的汛期安全。 相似文献
109.
多功能滚齿机的数字控制系统 总被引:1,自引:1,他引:0
本文叙述了以STDPC/XT(或PC/AT)机为主要核心构成的滚齿机数字控制系统的结构,软件设计和特点,系统采用混合语言编程,极大地提高了软件设计的灵活性与快速性,也分析了CNC系统的加工过程,提出了加工中的软件预处理控制方法,并给出了轴伺服控制的并行算法分析与实现。 相似文献
110.