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31.
The enzymic activity of the GTP-dependent acyl-CoA synthase isolated from rat liver mitochondria is affected by removal and
addition of lecithin (3). Purified preparations of this enzyme contain bound lecithin. In this presentation the nature of
the lecithin-protein interaction is examined. From binding measurements at different temperatures it is possible to postulate
which type of secondary valence bonds is holding the protein and lecithin together.
Presented at AOCS Meeting, Chicago, October 1967. 相似文献
32.
Gianni S Calosci N Aelen JM Vuister GW Brunori M Travaglini-Allocatelli C 《Protein engineering, design & selection : PEDS》2005,18(8):389-395
PDZ domains represent a large family of protein-interaction modules associated with a variety of unrelated proteins with different functions. We report a complete characterization of the kinetic folding mechanism of a fluorescent variant of PDZ2 from PTP-BL, investigated under a variety of different experimental conditions. For this purpose, we engineered a fluorescent variant of this protein Y43W (called pseudo-wild-type, pWT43). The results suggest the presence of a high-energy intermediate in the folding of PDZ2, as revealed by a pronounced non-linear dependence of the unfolding rate constant on denaturant concentration. Such an intermediate may or may not be detectable depending on the experimental conditions, giving rise to apparent two-state folding under stabilizing conditions (e.g. in the presence of sodium sulfate). Interestingly, even under these conditions, three-state folding can be restored by selectively destabilizing the native-like rate-limiting barrier by one specific mutation (V44A). Finally, we show that data taken on pWT43 under different experimental conditions (e.g. different pH values from 2.1 to 8.0 or in the presence of a stabilizing salt) and also data on a site-directed conservative mutant can be rationalized in terms of a simple reaction scheme involving a single set of intermediates and transition states. 相似文献
33.
Nisha Sheth Carl Greenley Raul Bermejo John C. Mauro Carlo G. Pantano Seong H. Kim 《Journal of the American Ceramic Society》2021,104(9):4550-4558
Water or acid soaking surface treatments have been shown to increase the mechanical strength of soda-lime silicate (SLS) glasses. This increase in strength has traditionally been attributed to effects related to residual stress or changes in fracture resistance. In this work, we report experimental data that cannot be explained based on the existing knowledge of glass surface mechanics. In dry environments, annealed and acid-leached SLS surfaces have comparable crack initiation stress and fracture stress as measured by Hertzian indentation and biaxial bending tests, respectively. Yet, in the presence of humidity, acid-leached surfaces have higher failure stress than the annealed surfaces. This apparent enhancement in the crack resistance of the acid-leached surface of SLS glass in humid environments supports the hypothesis that acid-leached surface chemistry can lower the transport kinetics of molecular water to critical flaws. 相似文献
34.
Anna Galotta Enrico Giust Mauro Bortolotti Gian D. Sorarù Vincenzo M. Sglavo Mattia Biesuz 《Journal of the American Ceramic Society》2021,104(9):4329-4340
Diatomite, a natural silicate-based sedimentary rock, was densified by cold sintering at room temperature and 150°C under various pressures (100, 200, and 300 MPa) and using different NaOH water solutions (0–3 M). The relative density of cold sintered diatomite can be as high as 90%, a condition that can be achieved by conventional firing only at 1200–1300°C. The cold sintered materials maintain the same mineralogical composition of the starting powder (quartz, glass, and illite) and are constituted by well-deformed and flattened grains oriented orthogonally to the applied pressure. Conversely, an evident phase evolution takes place upon conventional firing with the formation of cristobalite and mullite. The bending strength of cold sintered artifacts can exceed 40 MPa and increases to ≈80 MPa after post-annealing at 800°C, such mechanical strength is much larger than that of conventionally pressed samples sintered at 800°C, which is only ≈1 MPa. 相似文献
35.
Lorenzo Gatti Marco Guerini Oliviero Stock Carlo Strapparava 《Computational Intelligence》2015,31(4):669-683
The need for creativity is ubiquitous, and mobile devices connected to Web services can help us. Linguistic creativity is widely used in advertisements to surprise us, to get our attention, and to stick concepts in our memory. However, creativity can also be used as a defense. When we walk in the street, we are overwhelmed by messages that try to get our attention with any persuasive device at hand. As messages get ever more aggressive, often our basic cognitive defenses—trying not to perceive those messages—are not sufficient. One advanced defensive technique is based on transforming the perceived message into something different (for instance, making use of irony or hyperbole) from what was originally meant in the message. In this article, we describe an implemented application for smartphones, which creatively modifies the linguistic expression in a virtual copy of a poster encountered on the street. The mobile system is inspired by the subvertising practice of countercultural art. 相似文献
36.
37.
Ilaria Bartolini Vincenzo Moscato Ruggero G. Pensa Antonio Penta Antonio Picariello Carlo Sansone Maria Luisa Sapino 《Multimedia Tools and Applications》2016,75(7):3813-3842
The valorization and promotion of worldwide Cultural Heritage by the adoption of Information and Communication Technologies represent nowadays some of the most important research issues with a large variety of potential applications. This challenge is particularly perceived in the Italian scenario, where the artistic patrimony is one of the most diverse and rich of the world, able to attract millions of visitors every year to monuments, archaeological sites and museums. In this paper, we present a general recommendation framework able to uniformly manage heterogeneous multimedia data coming from several web repositories and to provide context-aware recommendation techniques supporting intelligent multimedia services for the users—i.e. dynamic visiting paths for a given environment. Specific applications of our system within the cultural heritage domain are proposed by means of real case studies in the mobile environment related both to an outdoor and indoor scenario, together with some results on user’s satisfaction and system accuracy. 相似文献
38.
Erik Sjöqvist Vahid Azimi Mousolou Carlo M. Canali 《Quantum Information Processing》2016,15(10):3995-4011
Geometric quantum computation is the idea that geometric phases can be used to implement quantum gates, i.e., the basic elements of the Boolean network that forms a quantum computer. Although originally thought to be limited to adiabatic evolution, controlled by slowly changing parameters, this form of quantum computation can as well be realized at high speed by using nonadiabatic schemes. Recent advances in quantum gate technology have allowed for experimental demonstrations of different types of geometric gates in adiabatic and nonadiabatic evolution. Here, we address some conceptual issues that arise in the realizations of geometric gates. We examine the appearance of dynamical phases in quantum evolution and point out that not all dynamical phases need to be compensated for in geometric quantum computation. We delineate the relation between Abelian and non-Abelian geometric gates and find an explicit physical example where the two types of gates coincide. We identify differences and similarities between adiabatic and nonadiabatic realizations of quantum computation based on non-Abelian geometric phases. 相似文献
39.
40.
Mirjana Mazuran Edoardo Serra Carlo Zaniolo 《The VLDB Journal The International Journal on Very Large Data Bases》2013,22(4):471-493
Supporting aggregates in recursive logic rules represents a very important problem for Datalog. To solve this problem, we propose a simple extension, called Datalog $^{FS}\,$ (Datalog extended with frequency support goals), that supports queries and reasoning about the number of distinct variable assignments satisfying given goals, or conjunctions of goals, in rules. This monotonic extension greatly enhances the power of Datalog, while preserving (i) its declarative semantics and (ii) its amenability to efficient implementation via differential fixpoint and other optimization techniques presented in the paper. Thus, Datalog $^{FS}\,$ enables the efficient formulation of queries that could not be expressed efficiently or could not be expressed at all in Datalog with stratified negation and aggregates. In fact, using a generalized notion of multiplicity called frequency, we show that diffusion models and page rank computations can be easily expressed and efficiently implemented using Datalog $^{FS}\,$ . 相似文献