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11.
1. At a holding potential of -40 mV, carbachol (50 microM) produced a complex pattern of inward currents in single smooth muscle cells freshly isolated from the mouse anococcygeus. Membrane currents were monitored by the whole-cell configuration of the patch-clamp technique. Previous work has identified the first, transient component as a calcium-activated chloride current (ICl(Ca)) and the second sustained component as a store depletion-operated non-selective cation current (I(DOC)). The object of the present study was to examine the cellular mechanisms underlying the third component, a series of inward current oscillations (I(oscil)) superimposed on I(DOC). 2. Carbachol-induced I(oscil) (amplitude 97 +/- 11 pA; frequency 0.26 +/- 0.02 Hz) was inhibited by the chloride channel blocker anthracene-9-carboxylic acid (A-9-C; 1 mM), and by inclusion of 1 mM EGTA in the patch-pipette filling solution. 3. In calcium-free extracellular medium (plus 1 mM EGTA), carbachol produced an initial burst of oscillatory current which lasted 94 s before decaying to zero; I(oscil) could be restored by re-admission of calcium. The frequency, but not the amplitude, of I(oscil) increased with increasing concentrations of extracellular calcium (0.5-10 mM). 4. Inclusion of the inositol triphosphate (IP3) receptor antagonist heparin (5 mg ml(-1) in the patch-pipette filling solution, or pretreatment of cells with the sarcoplasmic reticulum (SR) calcium ATPase inhibitor cyclopiazonic acid (CPA; 10 microM), prevented the activation of I(oscil) by carbachol. Caffeine (10 mM) activated both ICl(Ca) and I(DOC) and prevented the induction of I(oscil) by carbachol. Caffeine and CPA also abolished I(oscil) in the presence of carbachol, as did both a low (3 microM) and a high (30 microM) concentration of ryanodine. 5. Carbachol-induced I(oscil) was abolished by the general calcium entry blocker SKF 96365 (10 MM) and by Cd2+ (100 microM), but was unaffected by La3+ (400 microM). As found previously, I(DOC) was also blocked by SKF 96365 and Cd2+, but not La3+; the inhibition of I(DOC) preceded the abolition of I(oscil) by 27 s with SKF 96365 and by 30 s with Cd2+. Nifedipine (1 microM) produced a partial inhibition of the carbachol-induced I(oscil) frequency at holding potentials of -20 mV and -60 mV and, in addition, reduced I(DOC) at -60 mV by 18%. 6. It is concluded that carbachol-induced inward current oscillations in mouse anococcygeus cells are due to a calcium-activated chloride current, and reflect oscillatory changes in cytoplasmic calcium ion concentration. These calcium oscillations are derived primarily from the SR stores, but entry of calcium into the cell is necessary for store replenishment and maintenance of the oscillations. Capacitative calcium entry (via I(DOC) appears to be important not only for sustained contraction of this tissue, but also as a route for re-filling of the SR and, therefore, represents an important target for the development of novel and selective drugs. 相似文献
12.
GN Clarke 《Canadian Metallurgical Quarterly》1993,60(1):198-199
13.
KJ Clark AB Sarr PG Grant TD Phillips GN Woode 《Canadian Metallurgical Quarterly》1998,63(2-4):137-146
Rotaviruses are the leading cause and coronaviruses are the major contributors of acute gastroenteritis in the young of various mammalian and avian species. Despite numerous trials and decades of research, vaccines have limited efficacy particularly for calves. As an alternative method of controlling infection, we have investigated broad spectrum antiviral agents that are not discriminatory among various viruses. This report involves testing a variety of adsorbent agents including charcoal, clay, and clay minerals to adsorb rotavirus and coronavirus in vitro. Results revealed that all the adsorbent agents had good to excellent capability of adsorbing rotavirus and excellent capability of adsorbing coronavirus. Percent adsorptions ranged from 78.74% to 99.89% for rotavirus and 99.99% for coronavirus; while sand (negative control) was < 0.01%. A high affinity binding was present as determined by a low percent desorption (0.06-3.09%). However, the adsorbent bound virus complex retained, and may have actually enhanced, infectivity. 相似文献
14.
A constrained joint source/channel coder design 总被引:3,自引:0,他引:3
Sayood K. Fuling Liu Gibson J.D. 《Selected Areas in Communications, IEEE Journal on》1994,12(9):1584-1593
The design of joint source/channel coders in situations where there is residual redundancy at the output of the source coder is examined. It has previously been shown that this residual redundancy can be used to provide error protection without a channel coder. In this paper, this approach is extended to conventional source coder/convolutional coder combinations. A family of nonbinary encoders is developed which more efficiently use the residual redundancy in the source coder output. It is shown through simulation results that the proposed systems outperform conventional source-channel coder pairs with gains of greater than 9 dB in the reconstruction SNR at high probability of error 相似文献
15.
The formation of a porous polymer monolith (PPM) is influenced by the physico-chemical properties of the wall surface of its container. This influence can have a dramatic effect on the resulting monolith morphology depending on the nature and composition of the wall. Indeed, a dense polymer layer or “sheath”, distinct from the bulk porous material, has been observed at the wall surface of capillaries, and thus a study was undertaken to explore the dependence of this layer on the hydrophobicity of the surface. A range of silanizing reagents were used to modify the surface of the fused silica capillary, including aminopropyl, trimethylsilyl, octadecyl and perfluorooctyl functionalities. Crosslinked butyl acrylate-based PPM was formed in the modified capillaries and extruded. SEM images of the monoliths were used to examine the sheath morphology and thickness, which are discussed with respect to surface hydrophobicity. 相似文献
16.
E Gibson 《Canadian Metallurgical Quarterly》1998,68(1):1-76
This paper proposes a new theory of the relationship between the sentence processing mechanism and the available computational resources. This theory--the Syntactic Prediction Locality Theory (SPLT)--has two components: an integration cost component and a component for the memory cost associated with keeping track of obligatory syntactic requirements. Memory cost is hypothesized to be quantified in terms of the number of syntactic categories that are necessary to complete the current input string as a grammatical sentence. Furthermore, in accordance with results from the working memory literature both memory cost and integration cost are hypothesized to be heavily influenced by locality (1) the longer a predicted category must be kept in memory before the prediction is satisfied, the greater is the cost for maintaining that prediction; and (2) the greater the distance between an incoming word and the most local head or dependent to which it attaches, the greater the integration cost. The SPLT is shown to explain a wide range of processing complexity phenomena not previously accounted for under a single theory, including (1) the lower complexity of subject-extracted relative clauses compared to object-extracted relative clauses, (2) numerous processing overload effects across languages, including the unacceptability of multiply center-embedded structures, (3) the lower complexity of cross-serial dependencies relative to center-embedded dependencies, (4) heaviness effects, such that sentences are easier to understand when larger phrases are placed later and (5) numerous ambiguity effects, such as those which have been argued to be evidence for the Active Filler Hypothesis. 相似文献
17.
18.
19.
Synthesis and Characterisation of Some New Ferrocenyl- and Ferrocenylalkynyl-[60]Fullerene Compounds
David J. Cardin Trevor Gibson Jason A. Peel 《Fullerenes, Nanotubes and Carbon Nanostructures》1997,5(4):681-694
We report the synthesis and characterisation of a series of ferrocenylfullerene compounds, and some new ferrocene derivatives required as intermediates. The new fullerene species are Fc-[60]fullerene, (8); Fc-C=C-[60]fullerene, (10); Fc-C=C-C=C-[60]fullerene, (12); and (η-C5H4SiBu3)Fe(η-C5H4)-[60]fullerene, (14). 相似文献
20.
Critics of Kohlberg's moral theory today focus on the content of his theory and more specifically on its justice-orientated moral concept. This has led to the well-known 'justice-care debate'. The purpose of this article is to critically examine the validity of Kohlberg's moral theory for research in nursing ethics from a caring perspective (referring to the content) as well as from a cognitive-structural perspective (referring to the basic assumptions of the model). The analysis points to the usefulness and value of the cognitive-structural model to empirically study nurses' ethical behaviour; the content of Kohlberg's model, however, needs to be adapted by adding a caring perspective as well as some personal and situational variables. An adjusted version of Kohlberg's model is proposed and discussed. 相似文献