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71.
The effect of unsaturated fatty acids on the abundance of Δ6 desaturase (D6D) mRNA and the fatty acid composition of HepG2
cell membranes was examined. Supplementation of HepG2 cells with oleic acid (18:1n-9, OA), linoleic acid (18:2n-6, LA), α-linolenic
acid (18:3n-3, ALA), arachidonic acid (20:4n-6, AA) or eicosapentaenoic acid (20:5n-3, EPA) reduced D6D mRNA abundance by
39 ± 6.6, 40 ± 2.2, 31 ± 5.2, 55 ± 4.8, and 52 ± 5.0%, respectively, compared with control cells. Despite the reduction in
D6D mRNA abundance, the level of D6D conversion products (20:3n-9, EPA and AA) in OA, ALA and LA supplemented cells, respectively,
was elevated above that in control cells. Our results suggest that although unsaturated fatty acids decrease the abundance
of D6D mRNA by as much as 50%, the conversion of polyunsaturated fatty acids and accumulation of long chain polyunsaturated
fatty acids (LCPUFA) in HepG2 cell phospholipids continues to occur. 相似文献
72.
Flaxseed, echium, and canola oils contain α-linolenic acid (18∶3n−3, ALA) in a range of concentrations. To examine their effect
on elevating cardiac levels of long-chain n−3 FA, diets based on these n−3-containing vegetable oils were fed to rats for
4 wk. Sunflower oil, which contains little ALA, was a comparator. Despite canola oil having the lowest ALA content of the
three n−3-containing vegetable oils, it was the most potent for elevating DHA (22∶6n−3) levels in rat hearts and plasma. However,
the relative potencies of the dietary oils for elevation of EPA (20∶5n−3) in heart and plasma followed the same rank order
as their ALA content, i.e., flaxseed>echium>canola>sunflower oil. This paradox may be explained by lower ALA intake leading
to decreased competition for Δ6 desaturase activity between ALA and the 24∶5n−3 FA precursor to DHA formation. 相似文献
73.
Mixed linkage β-glucane and pentosanes (mainly arabinoxylanes) are the major endosperm cell-wall polysaccharides of barely and wheat respectively. These polysaccharides, although minor components of the whole grain, significantly affect the industrial utilization of these cereals. The modification of barley corns during malting requires the dissolution of the β-glucane in the cell-wall of the starch endosperm. High β-glucane concentration in wort and beer effect the rate of filtration and can also lead to precipitate or gel formation in the final product. In a similar manner, pentosane is thought to cause filtration problems with wheat starch hydrolysates by increasing viscosity and by producing gelatinous precipitate which blocks filters. Ironically, it is this same viscosity building and water binding capacity which is considered to render pentosanes of considerable value in dough development and bread storage (anti-staling functions). In the current paper, some aspects of the beneficial and detrimental effects of pentosanes and β-glucane in the industrial utilization of wheat and barley are discussed. More specifically, enzymic methods for the preparation, analysis and identification of these polysaccharides and for the removal of their functional properties, are described in detail. 相似文献
74.
Brian R. Gibson Erna Storgårds Kristoffer Krogerus Virve Vidgren 《Yeast (Chichester, England)》2013,30(7):255-266
Two distinct genetic groups (Saaz and Frohberg) exist within the hybrid Saccharomyces pastorianus (S. cerevisiae × S. eubayanus) taxon. However, physiological/technological differences that exist between the two groups are not known. Fermentative capability of the parental S. eubayanus has likewise never been studied. Here, 58 lager strains were screened to determine which hybrid group they belonged to, and selected strains were characterized to determine salient characteristics. In 15 °P all‐malt wort fermentations at 22 °C, Frohberg strains showed greater growth and superior fermentation (80% apparent attenuation, 6.5% alcohol by volume in 3–4 days) compared to all other strains and maintained highest viability values (>93%). Fermentation with S. eubayanus was poor at the same temperature (33% apparent attenuation, 2.7% alcohol by volume at 6 days and viability reduced to 75%). Saaz strains and S. eubayanus were the least sensitive to cold (10 °C), though this did not translate to greater fermentation performance. Fermentation with S. eubayanus was poor at 10 °C but equal to or greater than that of the Saaz strains. Performance of Saaz yeast/S. eubayanus was limited by an inability to use wort maltotriose. [14C]‐Maltotriose transport assays also showed negligible activity in these strains (≤0.5 µmol min?1 g?1 dry yeast). Beers from Saaz fermentations were characterized by two‐ to sixfold lower production of the flavour compounds methyl butanol, ethyl acetate and 3‐methylbutyl acetate compared to Frohberg strains. Higher alcohol and ester production by S. eubayanus was similar to that of Frohberg strains. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
75.
C. J. Bettles P. A. Lynch A. W. Stevenson D. Tomus M. A. Gibson K. Wallwork J. Kimpton 《Metallurgical and Materials Transactions A》2011,42(1):100-110
The strain evolution in polycrystalline CP-Ti strip under tension was studied in situ and at two length scales using Synchrotron X-ray diffraction. To establish the bulk material behavior, experiments were performed
at the Australian Synchrotron facility. Because of the relatively large grain size, discontinuous “spotty” Debye ring patterns
were observed, and a peak fitting algorithm was developed to determine the individual spot positions with the necessary precision
for strain determination. The crystallographic directional dependence of strain anisotropy during the loading cycle was determined.
Strain anisotropy and yielding of individual crystallographic planes prior to the macroscopic yield point were further clarified
by in situ loading experiments performed at the Advanced Light Source (ALS). The deviatoric strain accumulation and plastic response
were mapped on a grain-by-grain basis. The onset of microscopic yielding in the grains was identified and correlated with
the relative orientation of the grains with respect to the loading direction. 相似文献
76.
Equal access to mental health services is necessary for healthy individuals and communities. However, due to geographical distances and other barriers, some clients cannot easily access mental health professionals. Technologies such as videoconferencing for clinical purposes (i.e., telemental health) may help to bridge these gaps to connect clients and clinicians at geographically diverse locations. However, despite its potential utility, telemental health has not been widely adopted in Canada. This study is an exploratory investigation into mental health professionals' attitudes toward telemental health, factors that affect the frequency with which they use this technology, and their perceptions of individual characteristics that make clients more or less suitable candidates for telemental health. This study has a particular focus on remote and rural and Operational Stress Injury (OSI) contexts. One hundred sixty mental health workers across Canada participated in an online survey, and 25 mental health workers from Operational Stress Injury clinics across Canada participated in in-person interviews. The data were examined using qualitative and quantitative analysis methods. Findings suggest that mental health workers have overall positive attitudes toward the use of telemental health—particularly for clients in remote and rural locations. Additionally, receiving training in telemental health, being in the mental health field for longer, and perceiving the technology as easy to use are associated with more frequent use of telemental health. Finally, clinicians reported specific client characteristics that they perceive to make some clients unsuitable candidates for telemental health. Implications of these findings and directions for future research are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved) 相似文献
77.
Mentoring can have a significant positive impact on the lives of individuals. People with disabilities seeking to locate mentors face a variety of potential challenges and benefits. This article addresses mentoring-related issues faced by professionals with disabilities. The article presents a model of mentoring and discusses the difficulties faced by individuals with disabilities in locating mentors. It describes the negative impact of the lack of available mentors upon the educational and vocational development of people with disabilities. It offers effective strategies aimed at enhancing mentoring relationships with people with disabilities, including macrofocused strategies capable of enhancing the effectiveness of disability-related mentoring programs and microfocused strategies useful in developing quality mentoring relationships. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
78.
This paper measures technological change, factor demand and inter-factor and inter-fuel substitutability measures for China. We use individual fuel price data and a two-stage approach to estimate total factor cost functions and fuel share equations. Both inter-factor and inter-fuel substitution elasticities are calculated and the change in energy intensity is decomposed into its driving forces. The results suggest that energy is substitutable for capital regionally and for labor nationally. Capital substitutes for energy more easily than labor does. Energy intensity changes vary by region but the major drivers seem to be “budget effect” and the adoption of energy-intensive technologies, which might be embodied in high-level energy-using exports and sectors, capital investment and even old technique and equipment imports. Whether the trend in rising energy intensity continues will be significant for China and the rest of the world. 相似文献
79.
Kyriaki Polychronopoulou Nicholaos G. Demas Peter N. Gibson Claus Rebholz Andreas A. Polycarpou 《Tribology Letters》2010,38(1):57-68
Cu–Cr–N coatings with Cu contents between 3 and 65 at.%, Cu/Cr ratios in the 0.04–4.5 range and 21–27 at.% N, synthesized
by twin electron-beam Physical Vapor Deposition at 450 °C, were investigated and compared against substoichiometric Cr–N reference
samples. The main objective of this study is to study the influence of Cu on the structure, and the subsequent effects on
the mechanical properties, room (22 °C) and high temperature (500 and 840 °C) tribological performance of Cu–Cr–N coatings.
Using X-ray photoelectron spectroscopy, glancing angle X-ray diffraction and scanning electron microscopy, in combination
with nanoindentation mechanical property measurements and laboratory-controlled ball-on-disc sliding experiments, it is shown
that Cu–Cr–N coatings with low Cu content (3 at.%) possess sufficient wear resistance for high-temperature demanding tribological
applications. The lubricious effect of oxide formation at high temperatures is also evaluated. 相似文献
80.
Abuelmaatti A. Zafar J. Khairuddin I. Gibson A.A.P. Haigh A. Morgan I. 《Microwaves, Antennas & Propagation, IET》2009,3(2):242-249
Careful design of the magnetic bias circuit used in ferrite phase shifters can help to reduce space, weight and energy consumption. Low reluctance circuits must avoid air-gaps and this can be achieved using toroidal-shaped ferrite inserts positioned inside a rectangular waveguide. Here, these ferrite inserts are fabricated using a viscous plastic processing method that avoids machining and produces a continuous magnetic circuit. Finite-element methods are used to initially model the magnetostatic solution for the bias circuit before being used to analyse the microwave performance of a double toroid phase shifter. A closedform technique is introduced to model the dielectric slab waveguide impedance transformer. The final insertion loss was ,1 dB over the 9.5?10.3 GHz band and return loss of 20 dB was achievable. The phase shift calculation agrees to within 10% of the measured values. 相似文献