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111.
Caggiano G Puntillo F Coretti C Giglio M Alicino I Manca F Bruno F Montagna MT 《International journal of molecular sciences》2011,12(10):7038-7047
Multiple-site colonization with Candida spp. is commonly recognized as a risk factor for invasive fungal infection in critically ill patients. We carried out a study to determine the relationship between Candida colonization and invasive infection in neurological patients admitted to an ICU. At admission (T0) and every three days for two weeks, different samples (pharynx swab, tracheal secretions, stomach contents, etc.) were collected for mycological surveillance. Candida mannan antigen and Candida anti-mannan antibodies were assayed. The Colonization Index (CI) and Corrected Colonization Index were calculated for each time point. Of all patients 70% was already colonized by Candida spp. at T0 and six of them had CI ≥ 0.5. Three patients developed candidemia; they had CI ≥ 0.5 before infection. Positive values of Candida mannan antigen and anti-mannan antibodies were found only in the patients with candidemia. The sensitivity and specificity of the Candida mannan test were 66.6% and 100%, respectively, while the sensitivity and specificity of the anti-mannan antibody test were 100%. In accordance with other authors, we find the surveillance cultures are useful to monitor the Candida colonization in ICU patients. In addition, the sequential observation of anti-mannan antibodies could contribute to early diagnosis of candidiasis more than Candida mannan antigen in immunocompetent patients. 相似文献
112.
Somma S Pose G Pardo A Mulè G Pinto VF Moretti A Logrieco AF 《International journal of food microbiology》2011,145(2-3):414-419
Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760 mg/kg), alternariol monomethyl ether (AME, up to 1860 mg/kg), and alternariol (up to 70 mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease. 相似文献
113.
114.
Nanocomposite layers based on silica nanoparticles and a methacrylate matrix are synthesized by a solvent-free process and characterized in order to realize UV/Vis transparent optical waveguides. Chemical functionalization of the silica nanoparticles permits to interface the polymers and the silica. The refractive index, roughness and wettability and the machinability of the layers can be tuned changing the silica nanoparticle concentration and chemical modification of the surface of the nanoparticles. The optical transparency of the layers is affected by the nanoparticles organization between the organic chains, while it increased proportionally with respect to silica concentration. Nanocomposite layers with a concentration of 40 wt% in silica reached UV transparency for a wavelength of 250 nm. UV/Vis transparent waveguides were micromilled through nanocomposite layers and characterized. Propagation losses were measured to be around 1 dB cm(-1) at a wavelength of 350 nm. 相似文献
115.
Alessandra Zamparelli Nicoletta Zini Luca Cattini Giulia Spaletta Davide Dallatana Elena Bassi Fulvio Barbaro Michele Iafisco Salvatore Mosca Annapaola Parrilli Milena Fini Roberto Giardino Monica Sandri Simone Sprio Anna Tampieri Nadir M. Maraldi Roberto Toni 《Journal of materials science. Materials in medicine》2014,25(10):2421-2436
Few data are available on the effect of biomaterials on surface antigens of mammalian bone marrow-derived, adult mesenchymal stromal cells (MSCs). Since poly(l-lactic acid) or PLLA is largely used in tissue engineering of human bones, and we are developing a reverse engineering program to prototype with biomaterials the vascular architecture of bones for their bioartificial reconstruction, both in humans and animal models, we have studied the effect of porous, flat and smooth PLLA scaffolds on the immunophenotype of in vitro grown, rat MSCs in the absence of any coating, co-polymeric enrichment, and differentiation stimuli. Similar to controls on plastic, we show that our PLLA scaffold does not modify the distribution of some surface markers in rat MSCs. In particular, the maintained expression of CD73 and CD90 on two different subpopulations (small and large cells) is consistent with their adhesion to the PLLA scaffold through specialized appendages, and to their prominent content in actin. In addition, our PLLA scaffold favours retention of the intermediate filament desmin, believed a putative marker of undifferentiated state. Finally, it preserves all rat MSCs morphotypes, and allows for their survival, adhesion to the substrate, and replication. Remarkably, a subpopulation of rat MSCs grown on our PLLA scaffold exhibited formation of membrane protrusions of uncertain significance, although in a size range and morphology compatible with either motility blebs or shedding vesicles. In summary, our PLLA scaffold has no detrimental effect on a number of features of rat MSCs, primarily the expression of CD73 and CD90. 相似文献
116.
We present the design criteria and the basic structure of GYPSY, a software laboratory for visual inspection and recognition in industrial automation tasks.GYPSY analyzes images of objects on the basis of their two-dimensional shape. It is fully portable and easily programmable. The user can write application programs in a Pascal-like language to give the system the ability to take over various tasks.GYPSY has been developed for a multi-microprocessor architecture designed in Italy especially for industrial automation. 相似文献
117.
S. Marchetti P. Sandri R. Simili 《Journal of Infrared, Millimeter and Terahertz Waves》1997,18(7):1395-1409
In this work we report a complete theory of the metal-insulator-metal, antenna coupled, mm and sub-mm detectors. The voltage-power responsivity is simply derived from the geometrical antenna proprieties and the electrical junction characteristics. The calculated responsivity simply foresees our experimental results in the sub-mm — mm range and well fits all the previously reported data 相似文献
118.
119.
A Sandri L Besenzon A Acquaviva C Marino L Cordero di Montezemolo E Madon 《Canadian Metallurgical Quarterly》1998,15(6):557-561
OBJECTIVE: To evaluate whether the addition of bismuth subnitrate to a dual oral therapy regimen with omeprazole plus amoxycillin could improve Helicobacter pylori eradication. METHODS: Fifty consecutive Helicobacter pylori-positive patients were randomly enrolled to receive either (A) bismuth subnitrate (300 mg q.d.s.), omeprazole (20 mg b.d.) and amoxycillin (500 mg q.d.s.), or (B) omeprazole (20 mg b.d.) and amoxycillin (500 mg q.d.s.). Both groups (n=25 each) received the medication for 14 days. H. pylori status was reassessed 30 days after completion of the therapy in order to evaluate eradication rates. RESULTS: Six patients were lost to follow-up and therefore excluded from the study (three patients from each group). One patient from Group B withdrew from the study because of side-effects. The addition of bismuth subnitrate to omeprazole and amoxycillin significantly improved its efficacy in eradicating H. pylori, with 72% (18/25) eradication in Group A and 52% (13/25) in Group B (P=0.027). The addition of bismuth subnitrate to dual oral therapy was also capable of improving the healing of peptic ulcers when compared with dual oral therapy alone (100%, 8/8 vs. 58%, 4/7; P=0.021). CONCLUSION: Our results demonstrate that the addition of bismuth subnitrate to dual oral therapy enhances H. pylori eradication, and improves healing of peptic ulcers. 相似文献
120.
Landi E Logroscino G Proietti L Tampieri A Sandri M Sprio S 《Journal of materials science. Materials in medicine》2008,19(1):239-247
The incorporation of magnesium ions (in the range 5–10 mol% in respect to Ca) into the hydroxyapatite structure, which is
of great interest for the developing of artificial bone, was performed using magnesium chloride, calcium hydroxide and phosphoric
acid, as reactants. Among the synthesized powders, the synthetic HA powder containing 5.7% Mg substituting for calcium was
selected, due to its better chemico-physical features, and transformed into granules of 400–600 μm, for biocompatibility tests
(genotoxicity, carcinogenicity, toxicity, in vitro cytotoxicity and in vivo skin irritation-sensitization tests). In vivo
tests were carried out on New Zealand White rabbits using the granulate as filling for a femoral bone defect: osteoconductivity
and resorption were found to be enhanced compared to commercial stoichiometric HA granulate, taken as control. 相似文献