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71.
Gennady Verkhivker Steve Agajanian Ryan Kassab Keerthi Krishnan 《International journal of molecular sciences》2022,23(8)
In this study, we combine all-atom MD simulations and comprehensive mutational scanning of S-RBD complexes with the angiotensin-converting enzyme 2 (ACE2) host receptor in the native form as well as the S-RBD Delta and Omicron variants to (a) examine the differences in the dynamic signatures of the S-RBD complexes and (b) identify the critical binding hotspots and sensitivity of the mutational positions. We also examined the differences in allosteric interactions and communications in the S-RBD complexes for the Delta and Omicron variants. Through the perturbation-based scanning of the allosteric propensities of the SARS-CoV-2 S-RBD residues and dynamics-based network centrality and community analyses, we characterize the global mediating centers in the complexes and the nature of local stabilizing communities. We show that a constellation of mutational sites (G496S, Q498R, N501Y and Y505H) correspond to key binding energy hotspots and also contribute decisively to the key interfacial communities that mediate allosteric communications between S-RBD and ACE2. These Omicron mutations are responsible for both favorable local binding interactions and long-range allosteric interactions, providing key functional centers that mediate the high transmissibility of the virus. At the same time, our results show that other mutational sites could provide a “flexible shield” surrounding the stable community network, thereby allowing the Omicron virus to modulate immune evasion at different epitopes, while protecting the integrity of binding and allosteric interactions in the RBD–ACE2 complexes. This study suggests that the SARS-CoV-2 S protein may exploit the plasticity of the RBD to generate escape mutants, while engaging a small group of functional hotspots to mediate efficient local binding interactions and long-range allosteric communications with ACE2. 相似文献
72.
Noriah Bidin Siti Radhiana Azni Shumaila Islam Mundzir Abdullah M. Fakaruddin Sidi Ahmad Ganesan Krishnan A. Rahman Johari M. Aizat A. Bakar Nur Syahirah Sahidan NurFatin Musa M. Farizuddin Salebi Naqiuddin Razali Mohd Marsin Sanagi 《International Journal of Hydrogen Energy》2017,42(26):16325-16332
Hydrogen production via water electrolysis was studied under the effect of magnetic and optical field. A diode solid state laser at blue, green and red were utilized as optical field source. Magnetic bar was employed as external magnetic field. The green laser has shown a greatest effect in hydrogen production due to its non-absorbance properties in the water. Thus its intensity of electrical field is high enough to dissociation of hydronium and hydroxide ions during orientation toward polarization of water. The potential to break the autoprotolysis and generate the auto-ionization is the mechanism of optical field to reveal the hydrogen production in water electrolysis. The magnetic field effect is more dominant to enhance the hydrogen production. The diamagnetic property of water has repelled the present of magnetic in water. Consequently the water splitting occurs due to the repulsive force induced by the external magnetic field. The magnetic distributed more homogenous in the water to involve more density of water molecule. As a result hydrogen production due to magnetic field is higher in comparison to optical field. However the combination both fields have generated superior effect whereby the hydrogen yields nine times higher in comparison to conventional water electrolysis. 相似文献
73.
Sree Hari Krishnan Parthasarathi R. Padmanabhan Hema A. Murthy 《International Journal of Speech Technology》2011,14(4):361-368
This paper demonstrates the robustness of group delay based features to additive noise. First, we analytically show the robustness
of group delay based representations. The analysis makes use of the fact that, for minimum-phase signals, the group delay
function can be represented in terms of the cepstral coefficients of the log-magnitude spectrum. Such a representation results
in the speech spectrum dominating over the noise spectrum, both at low and high SNRs. Further, we experimentally demonstrate
the robustness of the representation on a voice activity detection (VAD) task, comparing a group delay based VAD algorithm
with standard VAD methods as well as a magnitude-spectrum based method. 相似文献
74.
L. Satyanarayan Anish Kumar T. Jayakumar C. V. Krishnamurthy Krishnan Balasubramaniam Baldev Raj 《Journal of Nondestructive Evaluation》2009,28(3-4):111-124
New techniques developed using the array transducer, to image and size the bottom-surface and near-through-wall crack-like defects in thick carbon steel components are discussed. Three studies are reported here including (a) Optimal beam steering angle for focused and unfocused inspection using phased array method for bottom-surface crack sizing. (b) A front wall correction algorithm for sizing of near-through-wall crack-like defects. (c) A small aperture technique for sizing of near-through-wall crack-like defects. A Finite Difference Time Domain (FDTD) based simulation was used to study and verify the experimental observations. The application of time domain scheme relative arrival time technique (RATT), to measure the size of the near-through wall crack-like defects for the leak before break (LBB) criterion, was also investigated and found to be insufficient. A conventional SAFT algorithm was used for improving the sizing using the small aperture technique. 相似文献
75.
We present transmission electron microscopy observations of domain wall motion in thin freestanding KNbO 3 crystals under applied electric fields. Since there is no substrate, there is no elastic clamping of 90 domains. We observe that curved and tilted 90 domain walls are the most mobile, whereas untilted 90 domain walls are resistant to field-induced motion. We explain this result in terms of two factors. First, the switching pressure on a domain wall ( P 2 m P 1 ) E is determined by the relative electrostatic energies of the neighboring polarizations P 1 and P 2 . Consequently, some 90 domain walls are immobile under certain field directions, leading to domain interlocking. Second, domain walls experiencing a high switching pressure move by a ripple mechanism, and do not move as rigid sheets. The tilted wall region in such a ripple has a polarization charge, and an associated depolarization field, which reduces the local switching barrier. An accumulation of polarization charge can result in a tilted or curved wall, as occurs at the mobile tips of 90 domain needles. Any increase in density of immobile wall configurations with cycle time represents an inherent contribution to fatigue. Uniaxial ferroelectrics, with polarizations parallel to the field, should not experience such domain interlocking. 相似文献
76.
Koel Das Aditi Majumder Monica Siegenthaler Hans Keirstead M. Gopi 《The Visual computer》2011,27(12):1055-1069
Remyelination therapy is a state-of-the-art technique for treating spinal cord injury (SCI). Demyelination—the loss of myelin
sheath that insulates axons, is a prominent feature in many neurological disorders resulting in SCI. This lost myelin sheath
can be replaced by remyelination. In this paper, we propose an algorithm for efficient automated cell classification and visualization
to analyze the progress of remyelination therapy in SCI. Our method takes as input the images of the cells and outputs a density
map of the therapeutically important oligodendrocyte-remyelinated axons (OR-axons) which is used for efficacy analysis of
the therapy. Our method starts with detecting cell boundaries using a robust, shape-independent algorithm based on iso-contour
analysis of the image at progressively increasing intensity levels. The detected boundaries of spatially clustered cells are
then separated using the Delaunay triangulation based contour separation method. Finally, the OR-axons are identified and
a density map is generated for efficacy analysis of the therapy. Our efficient automated cell classification and visualization
of remyelination analysis significantly reduces error due to human subjectivity. We validate the accuracy of our results by
extensive cross-verification by the domain experts. 相似文献
77.
Sreeraj Gopi Augustine Amalraj Karthik Varma Shintu Jude Prakash B. Reddy Chandradhara Divya 《国际聚合物材料杂志》2018,67(9):581-588
A phytogenic feed additive (PFA) formulation was prepared with bioactive molecules—curcuminoids, gingerol, and carvacrol by encapsulating in nanofiber isolated from turmeric spent (turmeric nanofiber, TNF). This formulation was completely characterized by scanning electron microscope, ultra performance liquid chromatography, and gas chromatography and evaluated for its efficacy. PFA-encapsulated TNF (PFA@TNF) considerably increased mean body weight, decreased cholesterol level, mortality rate, and reduced Escherichia coli content of broilers than antibiotic growth promoter (AGP). The depth of crypts in the ileum of broilers was considerably reduced by the inclusion of PFA@TNF in diets compared with the AGP. 相似文献
78.
79.
Krishnan R. Arumugan R. Lindsay J.F. 《Industry Applications, IEEE Transactions on》1988,24(3):456-461
Some of the design aspects of the switched-reluctance motor for nonservo applications are considered. Towards this objective, a step-by-step procedure is developed for the design of switched-reluctance motors 相似文献
80.
Krishnan R. Stefanovic Victor R. Lindsay James F. 《Industry Applications, IEEE Transactions on》1983,(1):94-104
Efficient use of an inverter-fed induction motor drive necessitates a knowledge of the characteristics of the induction motor from a " control" point of view. The characteristics of both the voltage -and the current-source inverter-fed induction motor drive are investigated, neglecting the filter time constants in the link and feedback loops. Of particular interest is the role played by the voltage/current and frequency inputs. Their effects on variables that are not often discussed such as torque angle, stator real power, airgap flux- linkages and stator and rotor voltages/currents are studied. A by- product of this aspect of the study leads to the nature of feedback signals and their suitability for control purposes. It is also found that the link filter has little effect on relative suitability of these signals. A study of the current source induction motor drive dynamics for torque angle feedback is included. 相似文献