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11.
Compatibilizing effects of diblock copolymer polystyrene-block-poly(ethylene-co-propylene) (SEP) on the morphology and mechanical properties of immiscible blends of poly(propylene) (PP) and polystyrene (PS) were investigated. Notched impact strength, yield stress, elongation at yield and Young's modulus were determined as a function of different weight ratios of PP and PS and different amounts of added SEP as well. Scanning electron microscopy revealed a two-phase morphology of PP/PS blends, which exhibit poor mechanical properties. Even 2,5 wt.-% of SEP added to PP/PS blends can improve the notched impact strength and elongation at yield compared to non-compatibilized PP/PS blends. 10 wt.-% of SEP compatibilizer converted the brittle PP/PS blend to quite impactresistant polymeric material. Mechanical properties were improved because of the morphological changes and increased interfacial adhesion as a result of SEP localization between PP and PS phases. An analysis of yield stress data in terms of theoretical models showed that yield stress values of binary PP/PS blends can be predicted with Nielsen's model. 相似文献
12.
Midon M Gimadutdinow O Meiss G Friedhoff P Pingoud A 《Chembiochem : a European journal of chemical biology》2012,13(5):713-721
The His-Asn-His (HNH) motif characterizes the active sites of a large number of different nucleases such as homing endonucleases, restriction endonucleases, structure-specific nucleases and, in particular, nonspecific nucleases. Several biochemical studies have revealed an essential catalytic function for the first amino acid of this motif in HNH nucleases. This histidine residue was identified as the general base that activates a water molecule for a nucleophilic attack on the sugar phosphate backbone of nucleic acids. Replacement of histidine by an amino acid such as glycine or alanine, which lack the catalytically active imidazole side chain, leads to decreases of several orders of magnitude in the nucleolytic activities of members of this nuclease family. We were able, however, to restore the activity of HNH nuclease variants (i.e., EndA (Streptococcus pneumoniae), SmaNuc (Serratia marcescens) and NucA (Anabaena sp.)) that had been inactivated by His→Gly or His→Ala substitution by adding excess imidazole to the inactive enzymes in vitro. Imidazole clearly replaces the missing histidine side chain and thereby restores nucleolytic activity. Significantly, this chemical rescue could also be observed in vivo (Escherichia coli). The in vivo assay might be a promising starting point for the development of a high-throughput screening system for functional EndA inhibitors because, unlike the wild-type enzyme, the H160G and H160A variants of EndA can easily be produced in E. coli. A simple viability assay would allow inhibitors of EndA to be identified because these would counteract the toxicities of the chemically rescued EndA variants. Such inhibitors could be used to block the nucleolytic activity of EndA, which as a surface-exposed enzyme in its natural host destroys the DNA scaffolds of neutrophil extracellular traps (NETs) and thereby allows S. pneumoniae to escape the innate immune response. 相似文献
13.
Prof. Dr. Frank Lipnizki Dr. Johan Thuvander Dr. Gregor Rudolph-Schöpping 《化学,工程师,技术》2023,95(9):1403-1408
Proteins are not only important for our daily diet but also one of the most important food ingredients worldwide. Since the 1970ies membrane processes have established themselves as a key unit operation in protein processing, e.g., in the dairy industry. The recent trend of the protein shift moving consumption of animal-based to plant-based proteins provides new applications for membrane processes. Thus, in this work, the opportunities to transfer the membrane processing experiences from animal-based proteins to plant-based protein products will be the focus. 相似文献
14.
Shin-ichiro Ohno Gregor P. C. Drummen Masahiko Kuroda 《International journal of molecular sciences》2016,17(2)
Many types of cells release phospholipid membrane vesicles thought to play key roles in cell-cell communication, antigen presentation, and the spread of infectious agents. Extracellular vesicles (EVs) carry various proteins, messenger RNAs (mRNAs), and microRNAs (miRNAs), like a “message in a bottle” to cells in remote locations. The encapsulated molecules are protected from multiple types of degradative enzymes in body fluids, making EVs ideal for delivering drugs. This review presents an overview of the potential roles of EVs as natural drugs and novel drug-delivery systems. 相似文献
15.
Dr. Kely Navakoski de Oliveira Vincent Andermark Susanne von Grafenstein Liliane A. Onambele Gregor Dahl Riccardo Rubbiani Prof. Dr. Gerhard Wolber Dr. Chiara Gabbiani Prof. Dr. Luigi Messori Prof. Dr. Aram Prokop Prof. Dr. Ingo Ott 《ChemMedChem》2013,8(2):256-264
Thioredoxin reductase (TrxR) is overexpressed in cancer cells and is therefore a putative cancer target. Inhibition of this enzyme is considered an important strategy for the development of new chemotherapeutic agents with a specific mechanism of action. Organotin compounds have been described as experimental antitumor agents, yet their mechanism of action remains largely unknown. Based on the outcome of a virtual screening study, various di‐ and tri‐n‐butyltin(IV) carboxylates were synthesized, and their biological properties were evaluated. All synthesized compounds were able to inhibit TrxR selectively within the micromolar range and showed potent antitumor activity against HT‐29 and MCF‐7 cancer cell lines. Moreover, tin(IV) organometallics were found to strongly induce apoptosis in the BJAB lymphoma cell line. Mass spectrometry and atomic absorption spectroscopy experiments revealed metal binding to proteins, and efficient cellular uptake was observed using a di‐n‐butyltin(IV) complex as an example. 相似文献
16.
Compatibilizing effects of ethylene/propylene (EPR) diblock copolymers on the morphology and mechanical properties of immiscible blends produced from recycled low‐density polyethylene (PE‐LD) and high‐density polyethylene (PE‐HD) with 20 wt.‐% of recycled poly(propylene) (PP) were investigated. Two different EPR block copolymers which differ in ethylene monomer unit content were applied to act as interfacial agents. The morphology of the studied blends was observed by scanning‐ (SEM) and transmission electron microscopy (TEM). It was found that both EPR copolymers were efficient in reducing the size of the dispersed phase and improving adhesion between PE and PP phases. Addition of 10 wt.‐% of EPR caused the formation of the interfacial layer surrounding dispersed PP particles with the occurrence of PE‐LD lamellae interpenetration into the layer. Tensile properties (elongation at yield, yield stress, elongation at break, Young's modulus) and notched impact strength were measured as a function of blend composition and chemical structure of EPR. It was found that the EPR with a higher content of ethylene monomer units was a more efficient compatibilizer, especially for the modification of PE‐LD/PP 80/20 blend. Notched impact strength and ductility were greatly improved due to the morphological changes and increased interfacial adhesion as a result of the EPR localization between the phases. No significant improvements of mechanical properties for recycled PE‐HD/PP 80/20 blend were observed by the addition of selected block copolymers. 相似文献
17.
18.
Tobias Rosenkranz Alexandros Katranidis Diaa Atta Ingo Gregor Dr. Jörg Enderlein Prof. Mariusz Grzelakowski Dr. Per Rigler Dr. Wolfgang Meier Prof. Jörg Fitter Dr. 《Chembiochem : a European journal of chemical biology》2009,10(4):702-709
Protein unfolding inside immobilized polymerosomes : One of the most interesting properties of polymeric vesicles is their remarkable stability against extreme temperatures and osmotic stress, and their longevity even under harsh environmental conditions. We have demonstrated, in an application on protein folding, that surface‐tethered polymerosomes are suitable for performing time‐resolved single molecule studies with encapsulated proteins, as illustrated here.
19.
20.
Marion Börnhorst Jens Friedland Bjarne Kreitz Emanuele Moioli Gregor Wehinger 《化学,工程师,技术》2021,93(8):1210-1213
In this article, the results of the NaWuReT (Early Career Reaction Engineers) workshop on the topic “Are we doing relevant science?” are presented. The topics “(In)surmountable hurdles for Citizen Scientists in reaction engineering?” and “Circular Economy in reaction engineering with/for society?” were discussed. Therefrom, a variety of ideas and suggestions were extracted. 相似文献