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101.
102.
提出了一种离线式基于时间门的荧光受激发射损耗(g-STED)显微方法。基于在强光照条件下荧光寿命缩短的理论模型,在常规STED架构基础上,使用时间相关单光子记数(TCSPC)算法获取图像的荧光寿命信息,离线设置合理的时间门阈值,丢弃短寿命信号数据,对荧光信号有效点扩展函数(PSF)进行压缩,达到超分辨显微的目的。与传统STED显微术相比,此方法所需光功率大幅度降低,减少了荧光漂白及光毒性;离线式处理则同时增加了时间门设置的灵活性。在实验中,使用45mW的连续STED光,最终获取了约80nm的图像空间分辨率。进一步对时间门的设置对获取图像信号的分辨率和信噪比的影响进行了讨论。 相似文献
103.
为集成调谐器接收机芯片系统设计了一个带自动幅度控制回路的差分结构电容电感压控振荡器.通过采用pMOS管作为有源负阻使振荡器谐振回路可以直接接地电平,减小了寄生效应,扩大了频率调谐的线性及其范围.采用的自动幅度控制AAC回路具有元件少,噪声低,控制灵敏,调节容易,结构简单及设计方便的优点,并保证振荡器电路的性能最小地依赖于环境和制造工艺参数的变化.所设计的压控振荡器采用新加坡特许50GHz 0.35μm SiGe BiCMOS工艺流片,经测试在1MHz频率偏移处达到了-127.27dBc/Hz的相位噪声性能,具有宽的(990~1140MHz)和线性(调谐增益32.4MHz/V)的频率调谐曲线.整个振荡器电路在5V的供电电压下仅消耗6.6mA的电流,可以满足调谐器的应用需要. 相似文献
104.
Shanguo Huang Weihua Lian Xian Zhang Bingli Guo Pei Luo Jie Zhang Wanyi Gu 《Photonic Network Communications》2012,23(2):183-190
The routing issues in multi-layer and multi-domain optical networks have drawn much attention in current research. With the
introduction of the path computation element, routes can be calculated more efficiently in multi-domain optical networks.
However, the optimal degree of routing approach in multi-layer and multi-domain optical networks is also determined by the
clustering algorithms deployed for construction of hierarchical networks. Therefore, it is important to investigate the way
to evaluate the impact of the clustering algorithm on the routing approach (e.g., blocking probability) in optical networks
with dynamic traffic, which has not been studied sufficiently. In this paper, a novel method to describe and evaluate the
clustered structures generated by different clustering algorithms for hierarchical optical networks is proposed. This method
deploys a novel evaluation metric that represents blocking probability of clustered optical networks, so it can be used as
guidelines for designing clustered structures. Besides theoretical analysis, simulations are carried out on different network
topologies and clustered types to validate the effectiveness of the method presented. 相似文献
105.
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107.
Unpassivated/passivated AlGaN/GaN high electron mobility transistors (HEMTs) were exposed to 1.25 MeV 60Co γ-rays at a dose of 1 Mrad(Si). The saturation drain current of the unpassivated devices decreased by 15% at 1 Mrad γ-dose, and the maximal transconductance decreased by 9.1% under the same condition; more- over, either forward or reverse gate bias current was significantly increased, while the threshold voltage is relatively unaffected. By sharp contrast, the passivated devices showed scarcely any change in saturation drain current and maximal transconductance at the same γ dose. Based on the differences between the passivated HEMTs and un- passivated HEMTs, adding the C–V measurement results, the obviously parameter degradation of the unpassivated AlGaN/GaN HEMTs is believed to be caused by the creation of electronegative surface state charges in sourcegate spacer and gate–drain spacer at the low dose (1 Mrad). These results reveal that the passivation is effective in reducing the effects of surface state charges induced by the 60Co γ-rays irradiation, so the passivation is an effective reinforced approach. 相似文献
108.
Cancer Cell Membrane‐Coated Gold Nanocages with Hyperthermia‐Triggered Drug Release and Homotypic Target Inhibit Growth and Metastasis of Breast Cancer 下载免费PDF全文
Huiping Sun Jinghan Su Qingshuo Meng Qi Yin Lingli Chen Wangwen Gu Zhiwen Zhang Haijun Yu Pengcheng Zhang Siling Wang Yaping Li 《Advanced functional materials》2017,27(3)
The cell‐specific targeting drug delivery and controlled release of drug at the cancer cells are still the main challenges for anti‐breast cancer metastasis therapy. Herein, the authors first report a biomimetic drug delivery system composed of doxorubicin (DOX)‐loaded gold nanocages (AuNs) as the inner cores and 4T1 cancer cell membranes (CMVs) as the outer shells (coated surface of DOX‐incorporated AuNs (CDAuNs)). The CDAuNs, perfectly utilizing the natural cancer cell membranes with the homotypic targeting and hyperthermia‐responsive ability to cap the DAuNs with the photothermal property, can realize the selective targeting of the homotypic tumor cells, hyperthermia‐triggered drug release under the near‐infrared laser irradiation, and the combination of chemo/photothermal therapy. The CDAuNs exhibit a stimuli‐release of DOX under the hyperthermia and a high cell‐specific targeting of the 4T1 cells in vitro. Moreover, the excellent combinational therapy with about 98.9% and 98.5% inhibiting rates of the tumor volume and metastatic nodules is observed in the 4T1 orthotopic mammary tumor models. As a result, CDAuNs can be a promising nanodelivery system for the future therapy of breast cancer. 相似文献
109.
Metal‐Organic Frameworks Derived Nanotube of Nickel–Cobalt Bimetal Phosphides as Highly Efficient Electrocatalysts for Overall Water Splitting 下载免费PDF全文
Liting Yan Lei Cao Pengcheng Dai Xin Gu Dandan Liu Liangjun Li Ying Wang Xuebo Zhao 《Advanced functional materials》2017,27(40)
The design of highly efficient, stable, and noble‐metal‐free bifunctional electrocatalysts for overall water splitting is critical but challenging. Herein, a facile and controllable synthesis strategy for nickel–cobalt bimetal phosphide nanotubes as highly efficient electrocatalysts for overall water splitting via low‐temperature phosphorization from a bimetallic metal‐organic framework (MOF‐74) precursor is reported. By optimizing the molar ratio of Co/Ni atoms in MOF‐74, a series of Cox Niy P catalysts are synthesized, and the obtained Co4Ni1P has a rare form of nanotubes that possess similar morphology to the MOF precursor and exhibit perfect dispersal of the active sites. The nanotubes show remarkable hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) catalytic performance in an alkaline electrolyte, affording a current density of 10 mA cm?2 at overpotentials of 129 mV for HER and 245 mV for OER, respectively. An electrolyzer with Co4Ni1P nanotubes as both the cathode and anode catalyst in alkaline solutions achieves a current density of 10 mA cm?2 at a voltage of 1.59 V, which is comparable to the integrated Pt/C and RuO2 counterparts and ranks among the best of the metal‐phosphide electrocatalysts reported to date. 相似文献
110.