Intraocular penetrating of amoxicillin

Experiments have been carried out on four adult ewes to determine the effects on duodenal motility of intravenous or intraduodenal infusion of glucose solution and intravenous injection of insulin. At blood glucose levels below 80 mg%, the mean frequency of duodenal contractions was 1.6/min, but following the establishment of hyperglycemia by either intraduodenal or intravenous glucose infusion, contraction frequency fell to 0.9/min. The strength of contraction was unaffected.     

Arteriovenous fistula of the jejunum associated with gastrointestinal hemorrhage in a dog

Hemorrhage from the gastrointestinal tract of a young dog resulted in melena with concurrent anemia. Exploratory laparotomy revealed the hemorrhage originated from an arteriovenous fistula in the jejunum. Resection of the abnormal part of the jejunum was curative. The arteriovenous fistula in the dog was probably congenital in origin, but may have been the result of gastrointestinal tract trauma.     

Primary spinal extradural hydatid disease: report of a case with magnetic resonance characteristics and pathological correlation

A rare case of hydatid disease of the lumbar extradural area and of the paravertebral muscles is reported. The patient underwent surgery, resulting in complete recovery. The limitations of computed tomography and the benefits of magnetic resonance imaging in forming a diagnosis are discussed. Magnetic resonance has been found to be an invaluable tool, not only in detecting the soft tissue extent of the disease but also in delineating the viability of hydatid cysts.     

Right superior bronchial artery arising from the right subclavian artery and accompanying nerve branches: an autopsy case

The beta CK2tes gene encodes a new variant of the tissue-specific regulatory beta-subunit of casein kinase 2 (CK2). The beta CK2tes open reading frame comprises nucleotide stretches encoding for the conservative polypeptide motifs characteristic for the CK2 beta-subunit including the Glu/Asp rich region responsible for regulation of CK2, C-terminal fragment responsible for binding to the catalytic alpha-subunit, and "zinc finger" motif. Unlike conserved sequences of CK2 beta-subunits in other organisms the beta CK2tes polypeptide has no autophosphorylation site or other putative phosphorylation sites. beta CK2tes is expressed only in testes, whereas beta CK2 expression is maximal at embryonic stages and is detected also in larvae. We suggest that beta CK2tes determines substrate specificity of CK2 and/or CK2 activity during spermatogenesis in Drosophila.     

Electrochemical assay of proteinase inhibitors using polycation-sensitive membrane electrode detection

OBJECTIVE: Past research has shown response biases to influence the accuracy of results from self-report measures. In pain assessment, where a percentage of patients have financial and other reasons to minimize or exaggerate psychological disturbance, it becomes especially important to identify the influence of response bias in self-report of adjustment. This study investigated the susceptibility of three commonly used self-report pain assessment measures to response bias. DESIGN: This study used a within-subjects (asymptomatic subjects) design with two experimental conditions and nonequivalent control group (chronic pain patients). SUBJECTS: Experimental group: 40 students enrolled in an occupational therapy program at a major southeastern United States university. Control group: 200 subjects referred to a multidisciplinary pain clinic at a major teaching hospital. MEASURES: Coping Strategies Questionnaire, Multidimensional Pain Inventory, and Pain Beliefs and Perceptions Inventory. RESULTS: With few exceptions, asymptomatic subjects scored significantly differently on these measures while portraying themselves as either coping well or coping poorly. In addition, when using the "coping poorly" response set, asymptomatic subjects reproduced scores similar to those of symptomatic chronic pain patients. CONCLUSION: The susceptibility to manipulation appeared constant across the three measures, a finding that highlighted the difficulties clinicians and researchers encounter in accurate interpretation of results from these measures in the absence of validity indicators. This study also emphasizes the ease with which subjects with sufficient motivation can present themselves in an untruthful and manipulative manner and can generate scores that are, on their own, difficult to distinguish from those of a group of typical chronic pain patients.     

Characterisation of G serotype dependent non-antibody inhibitors of rotavirus in normal mouse serum

Serotype specific (non-immunoglobulin) inhibitors of rotavirus have been identified in normal mouse serum obtained from BALB/c, CBA, and BL10 mice. Sialic acid was essential for the neutralising activity sera treated with the neuraminidase from Vibrio cholerae failed to neutralise rotavirus. G serotypes 4, 5, 7, 8, 9, and 10 were unaffected by the inhibitor(s) while G serotypes 1, 2, 6 and two G3 strains were neutralised to significant titres. Assessment of neutralisation of reassortants suggested that VP7 is the virus protein involved in the interaction although it remains possible that VP7 is influencing VP4 binding. Analysis of the sera by Western blot followed by virus overlay confirmed that binding is dependent on the presence of sialic acid. The human strain tested, Wa, bound to two (glyco) proteins (50 and 80 kDa) while the bovine strains tested, NCDV and UK bound to one (55 kDa) and two (36 and 55 kDa) proteins respectively. This indicates that while the bovine rotaviruses may bind to a common element, the human strain binds to clearly distinct proteins. We propose that these inhibitors interact with animal rotaviruses in a manner analogous to that by which they attach to target cells. The glycoprotein to which NCDV bound was purified and identified by N-terminal sequencing as murine alpha-1-anti-trypsin (MuAAT) and was confirmed to possess both neutralisation and anti-trypsin activity. Since MuAAT is known to possess only three N-linked glycans, identification and analysis of the actual virus-binding structure should now be possible.