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991.
The rough surface of Nd:YAG-laser rods exhibits a broad spectral absorption due to impurities. The optical density of the investigated surfaces was determined up to several percent in the near infrared and visible spectral range. The characteristics of the rough surface absorption was investigated. 相似文献
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994.
Saerens D Frederix F Reekmans G Conrath K Jans K Brys L Huang L Bosmans E Maes G Borghs G Muyldermans S 《Analytical chemistry》2005,77(23):7547-7555
The specificity and affinity characteristics of antibodies make them excellent probes in biosensor applications. Unfortunately, their large size, unstable behavior, and random immobilization properties create numerous problems. The single-domain antigen-binding fragment derived from heavy-chain antibodies of camelids (termed VHH) offers special advantages in terms of size, stability, and ease of generating different antibody constructs. In this study, we show the potential of those VHHs in sensing human prostate-specific antigen (hPSA) by SPR technology. Different VHH constructs were immobilized onto commercial and custom-built sensor surfaces by metal chelation, biotin-streptavidin interaction, or covalent coupling. The detection of subnanogram per milliliter hPSA concentrations could be attained on a covalently coupled three-dimensional dextran surface. Moreover, the ratio of different hPSA isoform concentrations could be assessed via a sandwich assay and resulted in the detection of clinically significant antigen concentrations within 15 min. In addition, for the first time, the intrinsic protein stability is presented as an important probe design factor, since our results reveal that higher intrinsic stability offers higher resistance to harsh regeneration conditions. In conclusion, we present VHHs as a novel class of biosensor probes rivaling conventional antibodies and their derived antibody fragments. 相似文献
995.
José?Pineda?de?GyvezEmail author Guido?Gronthoud Rachid?Amine 《Journal of Electronic Testing》2005,21(3):311-322
We present industrial results of a quiescent current testing technique suitable for RF testing. The operational method consists of ramping the power supply and of observing the corresponding quiescent current signatures. When the power supply is swept, all transistors are forced into various regions of operation. This has as advantage that the detection of faults is done for multiple supply voltages and corresponding quiescent currents, enhancing in this form the detectability of faults. We found that this method of structural testing yields fault coverage results comparable to functional RF tests making it a potential and attractive technique for production wafer testing due to its low cost, low testing times and low frequency requirements.José Pineda de Gyvez received the Ph.D. degree from the Eindhoven University of Technology. He is currently a principal scientist at Philips Research Laboratories, The Netherlands. Dr. Pineda was Associate Editor in IEEE Transactions on Circuits and Systems Part I and also Associate Editor for Technology in IEEE Transactions on Semiconductor Manufacturing. His research interests are in the general areas of design for manufacturability and analog signal processing.Guido Gronthoud received the electrical engineering degree from the Delft University in 1975. From 1976 to 1980 he worked at the Delft University on the design of Microwave systems. From 1980 he works with Philips. He has been working in the fields of circuit simulation and modelling for IC designs, CAD development for PCB design and electronic circuits and systems reliability. Since 1998 he is working on test innovation of digital and mixed-signal circuits. His interests are Defect Oriented Test, fault modeling and Process Related Test. He has authored and co-authored technical papers. 相似文献
996.
Schumpeter maintained that oscillations of macroeconomic variables are only the “secondary wave” of business cycles, a reflex of more fundamental “primary waves” at the microeconomic level caused by the innovative activity of entrepreneurs. Uniting Schumpeter’s concern for innovation with Keynes’ concern for uncertainty and expectations formation, this article focuses on the behaviour of entrepreneurs confronting uncertainty caused by innovation. Entrepreneurs’ behaviour is reconstructed by modelling the functioning of their cognitive processes when innovations appear. Recognition of the possibilities opened up by a successful innovation generates a state of optimism in the minds of single entrepreneurs, which eventually propagates to the whole economy triggering an investments upswing. Likewise, unsuccessful innovations can trigger a downswing. 相似文献
997.
Jonathan Gelati Antonino Rotolo Giovanni Sartor Guido Governatori 《Artificial Intelligence and Law》2004,12(1-2):53-81
In this paper we provide a formal analysis of the idea of normative co-ordination. We argue that this idea is based on the assumption that agents can achieve flexible co-ordination by conferring normative positions to other agents. These positions include duties, permissions, and powers. In particular, we explain the idea of declarative power, which consists in the capacity of the power-holder of creating normative positions, involving other agents, simply by proclaiming such positions. In addition, we account also for the concepts of representation, namely the representatives capacity of acting in the name of his principal, and of mandate, which is the mandatees duty to act as the mandator has requested. Finally, we show how the framework can be applied to represent the contract-net protocol. Some brief remarks on future research and applications conclude this contribution. 相似文献
998.
Argumentation Semantics for Defeasible Logic 总被引:3,自引:0,他引:3
999.
Boehme S Werner G Klare I Reissbrodt R Witte W 《Molecular nutrition & food research》2004,48(7):522-531
Antibiotic-resistant bacteria or their corresponding resistance determinants are known to spread from animals to humans via the food chain. We screened 20 vegetable foods for antibiotic-resistant coliform bacteria and enterococci. Isolates were directly selected on antibiotic-containing selective agar (color detection). Thirteen "common vegetables" (tomato, mushrooms, salad) possessed 10(4)-10(7) cfu/g vegetable of coliform bacteria including only few antibiotic-resistant variants (0-10(5) cfu/g). All seven sprout samples showed a some orders of magnitude higher contamination with coliform bacteria (10(7)-10(9) cfu/g) including a remarkable amount of resistant isolates (up to 10(7) cfu/g). Multiple resistances (up to 9) in single isolates were more common in sprout isolates. Resistant bacteria did not originate from sprout seeds. The most common genera among 92 isolates were: 25 Enterobacter spp. (19 E. cloacae), 22 Citrobacter spp. (8 C. freundii), and 21 Klebsiella spp. (9 K. pneumoniae). Most common resistance phenotypes were: tetracycline (43%), streptomycin (37%), kanamycin (26%), chloramphenicol (29%), co-trimoxazol (9%), and gentamicin (4%). The four gentamicin-resistant isolates were investigated in molecular details. Only three (chloramphenicol) resistant, typical plant-associated enterococci were isolated from overnight enrichment cultures. In conclusion, a contribution of sprouts contaminated with multiresistant, Gram-negative enterobacteria to a common gene pool among human commensal and pathogenic bacteria cannot be excluded. 相似文献
1000.
It is shown that IMG (interferometric monitoring of greenhouse gases) spectra recorded over African and Arabian deserts clearly contain the fingerprint of quartz-rich soils. We illustrate how this spectral signature can be exploited to devise a suitable cloud-detection scheme to identify which infrared observations are affected by clouds. As a by-product, the scheme also allows one to identify the most likely underlying emitting surface type and provides a suitable first guess for the surface emissivity to be used, e.g., for the retrieval of geophysical parameters from high-spectral-resolution infrared radiance from space. The analysis has focused on African deserts because of their intrinsic relevance to numerical weather prediction and Earth's climate. Desert areas, like oceans, are poorly covered by the world meteorological radiosonde network and therefore are geographical regions for which the global coverage capability of satellites soundings is expected to provide better initializations for numerical weather prediction than are now available. Application of the cloud-detection scheme to IMG spectra has been considered, which demonstrates the good performance of the method. 相似文献