Molecular recognition of the Lewis Y antigen by monoclonal antibodies

The murine monoclonal antibody BR55-2 is directed against thetumor-associated antigen Lewis Y oligosaccharide. The LewisY core antigen is a difucosylated structure consisting of fourhexose units. Analysis of binding profiles of lactoseries isomericstructures by BR55–2 suggest that the binding epitopeincludes the OH-4 and OH-3 groups of the ß-D-galactoseunit, the 6-CH₃ groups of the two fucose units and the N-acetylgroup of the subterminal ß-D-N-acetylglucosamine (ßDGlcNAc).To elucidate the molecular recognition properties of BR55–2for the Y antigen, BR55–2 was cloned, sequenced and itsthree-dimensional structure was examined by molecular modeling.The crystal structure of BR96, another anti-Lewis Y antibody,solved in complex with a nonoate methyl ester Lewis Y tetrasaccharide,and the lectin IV protein in complex with a Lewis b tetrasaccharidecore were used as a guide to probe the molecular basis for BR55–2antigen recognition and specificity. Our modeling study showsthat BR55–2 shares similar recognition features for thedifucosylated type 2 lactoseries Lewis Y structure observedin the BR96-sugar complex. We observe that a major source ofspecificity for the Lewis Y structure by anti-Y antibodies emanatesfrom interaction with the ß-D-N-acetylglucosamineresidue and the nature of the structures extended at the reducingsite of the fucosylated lactosoamine.     

Vartalaap: A distributed multicast communication system

With the increasing proliferation of computer networks and distributed systems, there is a growing number of applications using multicast communication. This paper presents the Vartalaap system developed at IIT, Bombay. Vartalaap is an hierarchical distributed system for multicast communication over a network, implemented in a hardware-independent fashion. The multicast is achieved without resorting to unnecessary broadcasting of messages over the network. Issues covered in this paper include the primitives for multicast, the multicast model and the system architecture. We discuss the implementation of Vartalaap and compare it with some other systems. We conclude with a discussion on the limitations of the current implementation and directions for future work.     

Electrical characteristics and reliability of UV transparent Si/sub 3/N/sub 4/ metal-insulator-metal (MIM) capacitors

In this paper, we discuss the electrical characteristics and reliability of UV transparent Si/sub 3/N/sub 4/ metal-insulator-metal (MIM) capacitors. We examine film thicknesses in the range of 55 to 25 nm with capacitance densities from 1.2 ff//spl mu/m/sup 2/ to 2.8 ff//spl mu/m/sup 2/, respectively, for single MIM capacitors. A new approach for projecting the dielectric reliability of these films extends the limits of maximum operating voltage. Accounting for temperature acceleration and area scaling, the projected lifetimes can be met for a wide range of operating conditions.     

Record bandwidth, spectrally flat coupling with microbend gratings in dispersion-tailored fibers

We demonstrate resonant grating couplers with the broadest bandwidth (565 nm) reported to date, using microbend gratings in dispersion-optimized few-mode fibers. Control of modal dispersion enables the demonstration of spectrally flat (< 0.25-dB ripple) coupling. This enables a compact (1 cm), broad-band (110 nm) variable optical attenuator, which is highly cost-effective, since device assembly simply involves pressing an as-drawn fiber between two corrugated blocks.     

Is 1-hexene epoxidation in TS-1 diffusion limited in different solvents?

PFG NMR diffusion measurements were carried out to determine the effect of solvent on intracrystalline reactant diffusivities and on 1-hexene epoxidation rates in TS-1 catalyst. Using n-hexane in silicalite as a mimic for the TS-1 system, the self-diffusivity of n-hexane in silicalite was found to be 24% higher in methanol solvent than in acetonitrile solvent and 45% higher than in acetone solvent. The presence of trace Al did not affect n-hexane diffusivity. Based on analysis of the Weisz modulus for a slab morphology, the 1-hexene epoxidation reaction in TS-1 was found to be diffusion limited only if the crystal size is at least 38 μm in the methanol system.     

Status of iodine deficiency in selected blocks of Kangra District, Himachal Pradesh

Using polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and EB staining technique, paraffin-embeded sections of 20 hydatidiform mole and 4 choriocarcinoma were detected in the exons 5 and 8 of p53 gene. The results showed that mutations of p53 gene were 0/9 in the normal chorionic villi, 6/20 (30%) in hydatidiform mole and 3/4 in choriocarcinoma. This study suggests that mutations of p53 gene may be an important event in human gestational trophoblastic neoplastigenesis and its progression.     

Activation of platelets exposed to shear stress in the presence of smoke extracts of low-nicotine and zero-nicotine cigarettes: the protective effect of nicotine.

Mainstream and sidestream smoke extracts of both high-tar and low-tar cigarettes have been shown to increase platelet activation directly and to sensitize them to further activation by exposure to mechanical stimuli such as shear stress. However, nicotine has an inhibitory effect on platelet activation, opposite to that of whole cigarette smoke extracts. To distinguish between the nicotine-dependent and non-nicotine-dependent effects of smoke, platelets were exposed to mainstream and sidestream smoke extracts of low-nicotine and zero-nicotine cigarettes in vitro under flow conditions comparable with the normal circulation, and their activation state was measured using a modified prothrombinase-based assay. Both low-nicotine and zero-nicotine extracts caused increased platelet activation upon exposure to shear stress, and they caused significantly greater activation than extracts from ordinary high-tar, high-nicotine cigarettes. That nicotine was crucial was confirmed by adding 50 nM nicotine (comparable with the nicotine level in smokers' plasma) to zero-nicotine smoke extracts and demonstrating a reduction in the shear-dependent rate of platelet activation of more than 75%.     

Dispersion-tailored few-mode fibers: a versatile platform for in-fiber photonic devices

In-fiber devices enable a vast array of critical photonic functions ranging from signal conditioning (amplification, dispersion control) to network management (add/drop multiplexers, optical monitoring). These devices have become mainstays of fiber-optic communication systems because they provide the advantages of low loss, polarization insensitivity, high reliability, and compatibility with the transmission line. The majority of fiber devices reported to date are obtained by doping, designing, or writing gratings in the core of a single-mode fiber (SMF). Thus, these devices use the fiber only as a platform for propagating light-the device effect itself is due to some extraneously introduced material or structure (dopants for amplification, gratings for phase matching, etc.) There exists another, relatively less explored degree of freedom afforded by fibers-the ability to copropagate more than one mode. Each mode may have a uniquely defined modal dispersion and propagation characteristic. In this paper, we will describe the variety of fiber devices enabled by few-mode fibers-fibers that typically support two to four modes with suitably tailored dispersive properties. We will show that the unique dispersive properties of various modes, in conjunction with the ability to couple between them with gratings, leads to devices that offer novel solutions for dispersion compensation, spectral shaping, and polarization control, to name a few.     

An implementation of distributed shared memory

Shared memory is a simple yet powerful paradigm for structuring systems. Recently, there has been an interest in extending this paradigm to non-shared memory architectures as well. For example, the virtual address spaces for all objects in a distributed object-based system could be viewed as constituting a global distributed shared memory. We propose a set of primitives for managing distributed shared memory. We present an implementation of these primitives in the context of an object-based operating system as well as on top of Unix.