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排序方式: 共有1268条查询结果,搜索用时 15 毫秒
41.
Gustavo González‐Neves Graciela Gil Guzmán Favre Milka Ferrer 《International Journal of Food Science & Technology》2012,47(5):900-909
The anthocyanin composition of Tannat red wines obtained with four winemaking techniques was evaluated in different years. The wines were elaborated with traditional maceration (TM), cold pre‐fermentative maceration, delayed extraction of anthocyanins and extended maceration. Two vinifications were carried out for each technique, employing 70 kg of grapes in each one. The anthocyanin composition of wines was analysed by high‐performance liquid chromatography–diode array detection. The results confirm the fundamental role of the grape composition on the anthocyanin composition of the wines. The winemaking had an important effect on the total concentration of anthocyanins and also may modify the anthocyanic profile of wines. The wines obtained with TM had higher or similar content of anthocyanins than those elaborated with the other techniques. Canonical discriminant analysis of data showed that vintage has a greater effect on anthocyanin concentration than winemaking techniques. 相似文献
42.
Ultra-high performance concrete and fiber reinforced concrete: achieving strength and ductility without heat curing 总被引:5,自引:2,他引:3
Kay Wille Antoine E. Naaman Sherif El-Tawil Gustavo J. Parra-Montesinos 《Materials and Structures》2012,45(3):309-324
Ultra-high performance concrete (UHPC) and ultra-high performance fiber reinforced concrete (UHP-FRC) were introduced in the
mid 1990s. Special treatment, such as heat curing, pressure and/or extensive vibration, is often required in order to achieve
compressive strengths in excess of 150 MPa (22 ksi). This study focuses on the development of UHP-FRCs without any special
treatment and utilizing materials that are commercially available on the US market. Enhanced performance was accomplished
by optimizing the packing density of the cementitious matrix, using very high strength steel fibers, tailoring the geometry
of the fibers and optimizing the matrix-fiber interface properties. It is shown that addition of 1.5% deformed fibers by volume
results in a direct tensile strength of 13 MPa, which is 60% higher than comparable UHP-FRC with smooth steel fibers, and
a tensile strain at peak stress of 0.6%, which is about three times that for UHP-FRC with smooth fibers. Compressive strength
up to 292 MPa (42 ksi), tensile strength up to 37 MPa (5.4 ksi) and strain at peak stress up to 1.1% were also attained 28 days
after casting by using up to 8% volume fraction of high strength steel fibers and infiltrating them with the UHPC matrix. 相似文献
43.
Gustavo Alberto Polenta Dorcas Weber Samuel Godefroy-Benrejeb Michael Abbott 《Food Analytical Methods》2012,5(2):216-225
The present study evaluates the effect of food processing on the antigenicity of pecan proteins as measured by enzyme-linked
immunosorbent assay (ELISA). In addition, proteomic tools were used to identify potential pecan markers suitable for confirming
the presence of pecan proteins in food and validating new methods developed to detect traces of the commodity. To assess the
effects of processing on protein stability and antigenicity, pecan nuts were submitted to heat treatments and extracts were
analysed by ELISA, sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot. The ELISA method was able
to detect pecan traces even after submitting the commodity to rigorous treatments, though these treatments affected the detectability
to varying degrees. Proteomic assessment showed that the majority of pecan proteins were matched by homology to walnut proteins,
which are more abundantly populated in the protein sequence databases. However, there were a few important exceptions: 7S vicilin, 11S legumin and putative allergen I1, unambiguously identified as pecan in origin. Interestingly, putative allergen I1 offered unique analytical advantages to be used as a pecan marker for validation and confirmation purposes. 相似文献
44.
In this work were determined triclosan and 4n‐nonylphenol in water from a reservoir that is used to provide water to a purification plant in an important city in Colombia. The analytical methodology was validated using solid‐phase extraction and analysis by gas chromatography‐mass spectrometry (GC‐MS). The analysis by GC‐MS showed good linearity in the range of 0.05–5 μg/L for both compounds. Recoveries from 79 to 109% and standard deviations of 2.5–7.7 for low concentrations and from 3.8 to 9.6 (n = 5) for high concentrations were obtained for both compounds. In Colombia, this is the first time that these compounds have been analysed in water supplying of a drinking water treatment plant. The validated method was applied to the analysis of 27 samples collected in August 2010 in 11 locations from the reservoir and in the influent and effluent of the drinking water treatment plant. In total, seven samples were found to contain triclosan. 相似文献
45.
Zlatica Marinkovi Giovanni Crupi Alina Caddemi Gustavo Avolio Antonio Raffo Vera Markovi Giorgio Vannini Dominique M. M.‐P. Schreurs 《International Journal of Numerical Modelling》2015,28(4):359-370
Gallium nitride high electron‐mobility transistors have gained much interest for high‐power and high‐temperature applications at high frequencies. Therefore, there is a need to have the dependence on the temperature included in their models. To meet this challenge, the present study presents a neural approach for extracting a multi‐bias model of a gallium nitride high electron‐mobility transistors including the dependence on the ambient temperature. Accuracy of the developed model is verified by comparing modeling results with measurements. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
46.
Gustavo F. Brito Pankaj Agrawal Edcleide M. Araújo Tomás J.A. Mélo 《Polymer Engineering and Science》2014,54(8):1922-1930
Blends of poly(lactic acid) (PLA) and ethylene/methyl acrylate/glycidyl methacrylate terpolymer (EMA‐GMA) with and without the addition of an organoclay were prepared by melt mixing in a twin screw extruder. Mechanical, morphological, structural, and rheological properties of the systems have been investigated as function of its compositions. The impact strength (IS) of PLA increased with the addition of EMA‐GMA. Furthermore, the addition of 2.5 wt% of organoclay to the PLA/EMA‐GMA blend promoted improvements in the mechanical properties, such as IS, tensile strength, and strain‐at‐break. Further addition of organoclay, 5 wt%, led to a formation of a double percolated network, where the clay particles form bridges across EMA‐GMA droplets and glue them together, however, without coalescence. In addition, morphological and wide‐angle X‐ray scattering analyses evidenced that the clay presents a partially exfoliated structure and that remains inside the EMA‐GMA droplets, probably as a consequence of the approach used to produce the systems. POLYM. ENG. SCI., 54:1922–1930, 2014. © 2013 Society of Plastics Engineers 相似文献
47.
Keuri E. Rodrigues Aline Azevedo Pricila R. Gonalves Maria H. B. Pontes Gustavo M. Alves Ruan R. Oliveira Cristine B. Amarante Joo P. M. Issa Raquel F. Gerlach Alejandro F. Prado 《International journal of molecular sciences》2022,23(5)
Atherogenic events promote changes in vessel walls, with alteration of the redox state, and increased activity of matrix metalloproteinases (MMPs). Thus, this study aims to evaluate aortic remodeling, MMP activity, and reactive oxygen species (ROS) levels after treatment with doxycycline in ApoE-⁄- and ovariectomized mice (OVX). Female ApoE-⁄--knockout mice (5 weeks) were submitted to ovariectomy surgery to induce experimental menopause. They then received chow enriched with 1% cholesterol to induce hypercholesterolemia. The animals were divided into two experimental groups: ApoE-⁄-/OVX vehicle and ApoE-⁄-/OVX doxycycline (30 mg/kg) administered by gavage once a day for 28 days (15th to the 18th week of life). Blood samples were collected to measure total cholesterol and fractions. The aorta was used for morphometry and to measure the activity and expression of MMP-2 and ROS levels. The ApoE-⁄-/OVX doxycycline group showed no change in total and fraction cholesterol levels. However, there was a reduction in ROS levels, MMP-2 expression, and activity that correlated with a decrease in atherosclerotic lesions relative to the ApoE-⁄-/OVX vehicle (p > 0.05). Therefore, we conclude that doxycycline in ApoE-⁄-/OVX animals promotes a reduction in atherosclerotic lesions by reducing ROS and MMP-2 activity and expression. 相似文献
48.
Marzuq A. Ungogo Gustavo D. Campagnaro Ali H. Alghamdi Manal J. Natto Harry P. de Koning 《International journal of molecular sciences》2022,23(5)
The animal trypanosomiases are infections in a wide range of (domesticated) animals with any species of African trypanosome, such as Trypanosoma brucei, T. evansi, T. congolense, T. equiperdum and T. vivax. Symptoms differ between host and infective species and stage of infection and are treated with a small set of decades-old trypanocides. A complication is that not all trypanosome species are equally sensitive to all drugs and the reasons are at best partially understood. Here, we investigate whether drug transporters, mostly identified in T. b. brucei, determine the different drug sensitivities. We report that homologues of the aminopurine transporter TbAT1 and the aquaporin TbAQP2 are absent in T. congolense, while their introduction greatly sensitises this species to diamidine (pentamidine, diminazene) and melaminophenyl (melarsomine) drugs. Accumulation of these drugs in the transgenic lines was much more rapid. T. congolense is also inherently less sensitive to suramin than T. brucei, despite accumulating it faster. Expression of a proposed suramin transporter, located in T. brucei lysosomes, in T. congolense, did not alter its suramin sensitivity. We conclude that for several of the most important classes of trypanocides the presence of specific transporters, rather than drug targets, is the determining factor of drug efficacy. 相似文献
49.
Gustavo D. Campagnaro Hamza A. A. Elati Sofia Balaska Maria Esther Martin Abril Manal J. Natto Fabian Hulpia Kelly Lee Lilach Sheiner Serge Van Calenbergh Harry P. de Koning 《International journal of molecular sciences》2022,23(2)
Toxoplasma gondii is unable to synthesize purines de novo, instead salvages them from its environment, inside the host cell, for which they need high affinity carriers. Here, we report the expression of a T. gondii Equilibrative Nucleoside Transporter, Tg244440, in a Trypanosoma brucei strain from which nucleobase transporters have been deleted. Tg244440 transported hypoxanthine and guanine with similar affinity (Km ~1 µM), while inosine and guanosine displayed Ki values of 4.05 and 3.30 µM, respectively. Low affinity was observed for adenosine, adenine, and pyrimidines, classifying Tg244440 as a high affinity oxopurine transporter. Purine analogues were used to probe the substrate-transporter binding interactions, culminating in quantitative models showing different binding modes for oxopurine bases, oxopurine nucleosides, and adenosine. Hypoxanthine and guanine interacted through protonated N1 and N9, and through unprotonated N3 and N7 of the purine ring, whereas inosine and guanosine mostly employed the ribose hydroxy groups for binding, in addition to N1H of the nucleobase. Conversely, the ribose moiety of adenosine barely made any contribution to binding. Tg244440 is the first gene identified to encode a high affinity oxopurine transporter in T. gondii and, to the best of our knowledge, the first purine transporter to employ different binding modes for nucleosides and nucleobases. 相似文献
50.
Gabriela Antonio-Andres Gustavo U. Martinez-Ruiz Mario Morales-Martinez Elva Jimnez-Hernandez Estefany Martinez-Torres Tania V. Lopez-Perez Laura A. Estrada-Abreo Genaro Patino-Lopez Sergio Juarez-Mendez Víctor M. Davila-Borja Sara Huerta-Yepez 《International journal of molecular sciences》2022,23(3)