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991.
SV Litvintsev 《Canadian Metallurgical Quarterly》1998,319(9):21-4, 95
The drug addiction spread and its speed in Russia endanger the health and future of a substantial part of this country's youth in the nearest prospect. Military service is not a serious obstacle for the drug spread, so its real scale far exceeds the official statistics. The author recommends some measures which, if realized, would lead to the elimination of the problem. 相似文献
992.
Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins 总被引:2,自引:0,他引:2
SV Litvinov M Balzar MJ Winter HA Bakker IH Briaire-de Bruijn F Prins GJ Fleuren SO Warnaar 《Canadian Metallurgical Quarterly》1997,139(5):1337-1348
The contribution of noncadherin-type, Ca2+-independent cell-cell adhesion molecules to the organization of epithelial tissues is, as yet, unclear. A homophilic, epithelial Ca2+-independent adhesion molecule (Ep-CAM) is expressed in most epithelia, benign or malignant proliferative lesions, or during embryogenesis. Here we demonstrate that ectopic Ep-CAM, when expressed in cells interconnected by classic cadherins (E- or N-cadherin), induces segregation of the transfectants from the parental cell type in coaggregation assays and in cultured mixed aggregates, respectively. In the latter assay, Ep-CAM-positive transfectants behave like cells with a decreased strength of cell-cell adhesion as compared to the parental cells. Using transfectants with an inducible Ep-CAM-cDNA construct, we demonstrate that increasing expression of Ep-CAM in cadherin-positive cells leads to the gradual abrogation of adherens junctions. Overexpression of Ep-CAM has no influence on the total amount of cellular cadherin, but affects the interaction of cadherins with the cytoskeleton since a substantial decrease in the detergent-insoluble fraction of cadherin molecules was observed. Similarly, the detergent-insoluble fractions of alpha- and beta-catenins decreased in cells overexpressing Ep-CAM. While the total beta-catenin content remains unchanged, a reduction in total cellular alpha-catenin is observed as Ep-CAM expression increases. As the cadherin-mediated cell-cell adhesions diminish, Ep-CAM-mediated intercellular connections become predominant. An adhesion-defective mutant of Ep-CAM lacking the cytoplasmic domain has no effect on the cadherin-mediated cell-cell adhesions. The ability of Ep-CAM to modulate the cadherin-mediated cell-cell interactions, as demonstrated in the present study, suggests a role for this molecule in development of the proliferative, and probably malignant, phenotype of epithelial cells, since an increase of Ep-CAM expression was observed in vivo in association with hyperplastic and malignant proliferation of epithelial cells. 相似文献
993.
994.
995.
The study aimed to ascertain the current use of respite-care services by families with children with a learning and/or a physical disability in a South London, UK, health authority, to estimate the type and extent of the unmet need, and to set standards for the provision of such services. Over 1200 families were sent an 11-page questionnaire. The results indicated that the child's level of dependency and the presence of behaviour and communication problems led to significantly higher levels of experienced stress among carers. This was also associated with a significantly greater use of respite care except by families of children with behavioural problems. The results showed that respite care was generally perceived as an inadequate service, though 88% of respondents had no knowledge of the existence of respite-care services. Recommendations are made in relation to information provision, choice, and flexibility for carers and for quality standards in general. 相似文献
996.
The recent discoveries of planets orbiting several 'mature' stars bring new life to the question of just how common other planetary systems might be. Observations of very young stars provide a way to address this question and suggest that a significant number of such stars harbour conditions appropriate for the formation of planetary systems like our own. 相似文献
997.
998.
A survey for citrus viroids was conducted in the major citrus commercial growing areas in Costa Rica. Screening of 36 sweet orange and 12 lemon trees resulted in the detection of members of four of the five citrus viroid groups as determined by nucleic acid hybridization using specific RNA probes and polymerase chain reaction (PCR) using specific oligonucleotide primers. CEVd, CVd-IIa, CVD-IIb and CVd-III viroids were found to be widespread in the three main regions of commercial citrus production. CVd-Ib was only found in lemon in Nicoya. 相似文献
999.
Jian-Wen Kao Xiangdong Wu Earl G. Hammond Pamela J. White 《Journal of the American Oil Chemists' Society》1998,75(7):831-835
Oils from soybeans with high or low contents of furanoid fatty acids were evaluated during storage for flavor intensity of
soybean oil (SBO) off-flavor, but no significant differences were found. In addition, the compound 3-methylnonane-2,4-dione
(MND), a breakdown product of furanoid fatty acids suggested by other researchers to contribute to reversion flavor of SBO,
was evaluated for its contribution to off-flavor. The compound was synthesized in the laboratory and purified by gas chromatography
(GC) on a Silar 10 C column. GC analysis of the purified MND on a nonpolar SPB-1 column showed two well-separated main peaks
that have been suggested to represent keto and enol forms. Between these two peaks, a bridge of poorly resolved compounds
may have represented various possible enol forms or an equilibration among these forms during the GC separation. MND had an
intense straw-like and frulty odor when evaluated at the outlet of a gas chromatograph. Sensory evaluation of MND in a mineral
oil/water emulsion system showed that its flavor intensity increased almost imperceptibly with increased concentration (from
0.09 to 2.56 ppm). An explanation for this unusual flavor response may be that, when molecularly dispersed in air, MND has
an intense odor, but when placed in a mineral oil or soybean oil emulsion, MND may exist in a form with relatively low flavor
intensity, or it may be bound by the media. The concentrations of MND in SBO at various peroxide values were measured at 0
to 0.804 ppb, which were far less than concentrations tested in mineral oil/water emulsions during sensory evaluation and
below published odor threshold values for MND in oil. Therefore, these results do not support the theory that furanoid fatty
acids or MND contribute strongly to the reversion flavor of SBO.
This is Journal Paper J.17472 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 3396. 相似文献
1000.