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271.
Purv J. Purohit De-Yi Wang Franziska Emmerling Andreas F. Thünemann Gert Heinrich Andreas Schönhals 《Polymer》2012,53(11):2245-2254
Organically modified ZnAl Layered Double Hydroxides (ZnAl-LDH) was synthesized and melt blended with polyethylene to obtain nanocomposites. The resulting morphology was investigated by a combination of Differential Scanning Calorimetry (DSC), Small and Wide-angle X-ray scattering (SAXS and WAXS) and dielectric relaxation spectroscopy (DRS). The arrangement (intercalation) of polyethylene chains between LDH stacks was investigated employing SAXS. The homogeneity of the nanocomposites and average number of stack size (4–6 layers) were determined using scanning microfocus SAXS (BESSY II). DSC and WAXS results show that the degree of crystallinity decreases linearly with the increasing content of LDH. The extrapolation of this dependence to zero estimates a limiting concentration of ca. 45% LDH where the crystallization of PE is completely suppressed by the nanofiller. The dielectric spectra of the nanocomposites show several relaxation processes which are discussed in detail. The intensity of the dynamic glass transition (β-relaxation) increases with the concentration of LDH. This is attributed to the increasing concentration of the exchanged anion sodium dodecylbenzene sulfonate (SDBS) which is adsorbed at the LDH layers. Therefore, a detailed analysis of the β-relaxation provides information about the structure and the molecular dynamics in the interfacial region between the LDH layers and the polyethylene matrix which is otherwise dielectrically invisible (low dipole moment). 相似文献
272.
The complete transfer of multiwalled carbon nanotubes (MWCNTs) from a poly(styrene acrylonitrile) (SAN) precompound into an initially unfilled polycarbonate (PC) phase during 5 min of discontinuous melt mixing in a microcompounder has been observed recently. However, the typical time scale of the occurring transfer is presently still unknown. Morphological investigations within our study revealed that similar results can be obtained when the SAN-MWCNT-precompound is melt blended with neat PC by continuous twin-screw extrusion. Additionally, the kinetics of the CNT transfer was also investigated in a kneading chamber. We found that the CNT transfer rate turned out to be strongly coupled to the development of the interfacial area of the blend. Furthermore, we evaluated the interplay and significance of the possible and presently only insufficiently understood transfer mechanisms. We proposed for the first time a correlation between major processing and blend parameters and the probability of carbon nanotube transfer due to the development of a nanoscaled wetting angle. 相似文献
273.
Shirasuna K Kobayashi A Nitta A Nibuno S Sasahara K Shimizu T Bollwein H Miyamoto A 《Reproduction (Cambridge, England)》2012,143(4):491-500
The development of the corpus luteum (CL), which secretes large amounts of progesterone to establish pregnancy, is accompanied by active angiogenesis, vascularization, and lymphangiogenesis. Negative feedback regulation is a critical physiological mechanism. Vasohibin-1 (VASH1) was recently discovered as a novel endothelium-derived negative feedback regulator of vascularization. We therefore investigated the expression of VASH1 in the bovine CL. Expression of VASH1 mRNA and protein was predominantly localized to luteal endothelial cells (LECs). VASH1 expression in the CL was constant through the early to late luteal phases and decreased during CL regression relating with the action of luteolytic prostaglandin F(2)(α) in vivo. To investigate the role of VASH1, we determined whether VASH1 treatment affects angiogenesis and/or lymphangiogenesis using LECs and lymphatic endothelial cells (LyECs) in vitro. Vascular endothelial growth factor A (VEGFA) stimulated the expression of VASH1 in LECs but not in LyECs, and VASH1 completely blocked VEGFA-induced formation of capillary-like tube structures of LECs and LyECs in vitro. In summary, VASH1 is predominantly located on LECs in the bovine CL and inhibits the angiogenic and lymphangiogenic actions of VEGFA. Bovine CL therefore has a VEGFA-VASH1 system that may be involved in regulation of luteal function, especially in the development of the CL. The results indicate that VASH1 has the potential to act as a negative feedback regulator of angiogenesis and lymphangiogenesis in the CL in cows. 相似文献
274.
Close-packed nanoparticle monolayers have recently been shown to form mechanically robust, free-standing membranes. We report the first measurements of molecular transport through such ultrathin sheets, self-assembled from dodecanethiol-ligated gold nanocrystals. For aqueous solutions we find filtration coefficients 2 orders of magnitude larger than those observed in polymer-based filters, sieving of large solutes, and for smaller solutes a pronounced dependence of rejection on being charged. These results open up new possibilities for controlled delivery and separation of nano-objects. 相似文献
275.
Nanomechanical cantilever sensors have been emerging as a key device for real-time and label-free detection of various analytes ranging from gaseous to biological molecules. The major sensing principle is based on the analyte-induced surface stress, which makes a cantilever bend. In this letter, we present a membrane-type surface stress sensor (MSS), which is based on the piezoresistive read-out integrated in the sensor chip. The MSS is not a simple "cantilever," rather it consists of an "adsorbate membrane" suspended by four piezoresistive "sensing beams," composing a full Wheatstone bridge. The whole analyte-induced isotropic surface stress on the membrane is efficiently transduced to the piezoresistive beams as an amplified uniaxial stress. Evaluation of a prototype MSS used in the present experiments demonstrates a high sensitivity which is comparable with that of optical methods and a factor of more than 20 higher than that obtained with a standard piezoresistive cantilever. The finite element analyses indicate that changing dimensions of the membrane and beams can substantially increase the sensitivity further. Given the various conveniences and advantages of the integrated piezoresistive read-out, this platform is expected to open a new era of surface stress-based sensing. 相似文献
276.
Jens Bolten Thorsten WahlbrinkMathias Schmidt Heinrich D.B. GottlobHeinrich Kurz 《Microelectronic Engineering》2011,88(8):1910-1912
An electron beam lithography exposure strategy combining efficient proximity effect correction (PEC) with multi-pass grey scale is compared with a more conventional strategy based on a single-pass exposure without PEC. Exposure results for different types of nanowire structures are presented. Line edge and line width roughness (LER and LWR) values for both approaches are determined. The novel exposure strategy presented in this work reduced LER by ∼25% and LWR by ∼40%. 相似文献
277.
Murphy K van Ginneken B Reinhardt JM Kabus S Ding K Deng X Cao K Du K Christensen GE Garcia V Vercauteren T Ayache N Commowick O Malandain G Glocker B Paragios N Navab N Gorbunova V Sporring J de Bruijne M Han X Heinrich MP Schnabel JA Jenkinson M Lorenz C Modat M McClelland JR Ourselin S Muenzing SE Viergever MA De Nigris D Collins DL Arbel T Peroni M Li R Sharp GC Schmidt-Richberg A Ehrhardt J Werner R Smeets D Loeckx D Song G Tustison N Avants B Gee JC Staring M Klein S Stoel BC Urschler M 《IEEE transactions on medical imaging》2011,30(11):1901-1920
EMPIRE10 (Evaluation of Methods for Pulmonary Image REgistration 2010) is a public platform for fair and meaningful comparison of registration algorithms which are applied to a database of intrapatient thoracic CT image pairs. Evaluation of nonrigid registration techniques is a nontrivial task. This is compounded by the fact that researchers typically test only on their own data, which varies widely. For this reason, reliable assessment and comparison of different registration algorithms has been virtually impossible in the past. In this work we present the results of the launch phase of EMPIRE10, which comprised the comprehensive evaluation and comparison of 20 individual algorithms from leading academic and industrial research groups. All algorithms are applied to the same set of 30 thoracic CT pairs. Algorithm settings and parameters are chosen by researchers expert in the configuration of their own method and the evaluation is independent, using the same criteria for all participants. All results are published on the EMPIRE10 website (http://empire10.isi.uu.nl). The challenge remains ongoing and open to new participants. Full results from 24 algorithms have been published at the time of writing. This paper details the organization of the challenge, the data and evaluation methods and the outcome of the initial launch with 20 algorithms. The gain in knowledge and future work are discussed. 相似文献
278.
Macuhová J Tancin V Kraezl WD Meyer HH Bruckmaier RM 《The Journal of dairy research》2002,69(1):63-73
The aim of this study was to test if the opioid antagonist naloxone has a beneficial effect on normalization of oxytocin (OT) release during repeated milking of cows in unfamiliar surroundings. One control milking without naloxone treatment in all cows was performed in the familiar parlour. For four successive evening milkings, cows were transported to, and milked in, the operating theatre of the research station without (control group) or with naloxone administration (1 mg/kg BW) (naloxone group) before milking. After cessation of spontaneous milk flow, but not before 3 min of milking, vaginal stimulation was applied for 2 min. After milk flow ceased again, 10 IU of OT was injected intravenously to remove the remaining milk including residual milk. Milk flow was recorded continuously and blood samples were collected via a jugular vein cannula at 1-min intervals from 1 min before the start of milking until i.v. injection of OT. The inhibition of milk ejection and its normalization during repeated milking in unfamiliar surroundings was not influenced by naloxone treatment. Concentrations of cortisol and beta-endorphin during control milking and all relocations were similar in the naloxone and control groups, although their concentrations were higher after relocations than in the control. Therefore, a role of endogenous opioids in the inhibition of milk ejection in unfamiliar surroundings could not be demonstrated. In addition, the effect of exogenous ACTH1-24 (8 IU, i.v.) on the release of cortisol related to the response of cows milked in unfamiliar surroundings was studied. Cows with totally inhibited milk ejection in response to vaginal stimulation during milking after first relocation had numerically, but not significantly lower cortisol levels (8.8+/-3.4 ng/ml; AUC/min) in response to ACTH than did cows with at least partial milk ejection (38.7+/-12.9 ng/ml). Thus animals with a higher adrenal response to ACTH seemed to have less severe inhibition of milk ejection. 相似文献
279.
Ulrich Schillinger Claudia Guigas Wilhelm Heinrich Holzapfel 《International Dairy Journal》2005,15(12):1289-1297
This study compared selected probiotic properties of 9 strains of the Lactobacillus acidophilus group (6 L. acidophilus and 3 L. johnsonii) and 9 strains of the L. casei group, isolated from probiotic dairy products sold on the German market or supplied by a producer of starter cultures. Using an in vitro system simulating gastric transit, strains of the L. acidophilus group were more tolerant to the low pH 2.0 than strains of L. paracasei and L. rhamnosus which rapidly lost their viability after exposure to the simulated gastric juice containing pepsin. Milk showed a protective effect on strains less resistant to the gastric acidity. Bile salt hydrolase activity was detected in all strains of L. acidophilus/L. johnsonii but not in strains of the L. casei group. All lactobacilli were able to adhere to mucus secreting HT29 MTX cells and to human collagen type IV, fibrinogen and fibronectin although differences in the percentage of adhering bacteria were observed between different strains. 相似文献
280.
Heinrich U Koop U Leneveu-Duchemin MC Osterrieder K Bielfeldt S Chkarnat C Degwert J Häntschel D Jaspers S Nissen HP Rohr M Schneider G Tronnier H 《International journal of cosmetic science》2003,25(1-2):45-53
A multicentre study for measuring skin hydration with 349 volunteers was carried out in six different laboratories. The purpose of the study was to investigate physical-, physiological- and product-dependent parameters of three test emulsions (base, base + moisturizer and base + moisturizer + lipids) in a double-blind study. A comparison between analogous and digital sensor technology of the Corneometer CM825 was examined. Here, a clear relationship between both sensor types could be highlighted. A vital point of the study was the division of the test subjects according to their skin type. To get more objective limits for three different skin types - very dry, dry and normal skin - visual expert evaluation, self-assessment and hydration measurements were analysed by means of statistical methods. The moisture-related skin types were determined as follows: very dry skin was characterized with corneometer units below 30, dry skin between 30 and 40 and normal skin higher than 40 a.u. (arbitrary units). The efficacy of the three test emulsions was examined in relation to the mentioned skin types. Analysing the measured data of all test centres, a clear dependency of skin physiology (skin type) and product efficacy became evident. The drier the skin, the higher the increase of hydration. The product performance of the three test emulsions compared to the untreated control resulted in a significant increase of skin hydration in all measuring centres. The evaluation of a product ranking showed a good differentiation between the basic emulsion and the two other products. An increase of efficacy by adding lipids could be observed in four of six centres. The important influence of the skin type of the volunteers on the degree of product performance, as demonstrated in this study, should be especially considered when drawing up guidelines for efficacy testing. 相似文献