Evaluation of a type 1 diabetes serum cohort by SELDI-TOF MS protein profiling

Proteomics analysis of serum from patients with type 1 diabetes (T1D) may lead to novel biomarkers for prediction of disease and for patient monitoring. However, the serum proteome is highly sensitive to sample processing and before proteomics biomarker research serum cohorts should preferably be examined for potential bias between sample groups. SELDI‐TOF MS protein profiling was used for preliminary evaluation of a biological‐bank with 766 serum samples from 270 patients with T1D, collected at 18 different paediatric centers representing 15 countries in Europe and Japan over 2 years (2000–2002). Samples collected 1 (n = 270), 6 (n = 248), and 12 (n = 248) months after T1D diagnosis were grouped across centers and compared. The serum protein profiles varied with collection site and day of analysis; however, markers of sample processing were not systematically different between samples collected at different times after diagnosis. Three members of the apolipoprotein family increased with time in patient serum collected 1, 6, and 12 months after diagnosis (ANOVA, p<0.001). These results support the use of this serum cohort for further proteomic studies and illustrate the potential of high‐throughput MALDI/SELDI‐TOF MS protein profiling for evaluation of serum cohorts before proteomics biomarker research.     

A rigorous comparison of the Ewald method and the fast multipole method in two dimensions

The most efficient and proper standard method for simulating charged or dipolar systems is the Ewald method, which asymptotically scales as where N is the number of charges. However, recently the “fast multipole method” (FMM) which scales linearly with N has been developed. The break-even of the two methods (that is, the value of N below which Ewald is faster and above which FMM is faster) is very sensitive to the way the methods are optimized and implemented and to the required simulation accuracy.In this paper we use theoretical estimates and simulation results for the accuracies to carefully compare the two methods with respect to speed. We have developed and implemented highly efficient algorithms for both methods for a serial computer (a SPARCstation ELC) as well as a parallel computer (a T800 transputer based MEIKO computer). Breakevens in the range between N = 10 000 and N = 30 000 were found for reasonable values of the average accuracies found in our simulations. Furthermore, we illustrate how huge but rare single charge pair errors in the FMM inflate the error for some of the charges.     

Photocatalytic Valorization of Plastic Waste over Zinc Oxide Encapsulated in a Metal–Organic Framework

The conversion of waste plastic into high-value-added chemicals is regarded as a promising approach for relieving global plastic pollution and contributing to the circular economy. Herein, a partial calcination strategy is developed to fabricate a zinc oxide/UiO66-NH₂ (ZnO/UiO66-NH₂) heterojunction, in which ZnO is encapsulated in porous UiO66-NH₂ for the photocatalytic valorization of plastic. This strategy preserves the framework structure of UiO66-NH₂, thus enabling the formation of ZnO with ultra-small size distributed inside the skeleton. The synergistic effect of the obtained ZnO/UiO66-NH₂ heterojunction facilitates providing an efficient channel for carrier/mass transfer and guarantees structural stability. As a result, ZnO/UiO66-NH₂ exhibits high activity for converting polylactic acid (PLA) and polyvinyl chloride (PVC) into acetic acid, coupled with H₂ production. This work provides a feasible strategy for rationally designing heterojunction photocatalysts, as well as an insight into understanding the process of photocatalytic valorization of plastic.     

Integratable non-clogging microconcentrator based on counter-flow principle for continuous enrichment of CaSki cells sample

This study addresses the need to reduce the risk of clogging when preparing samples for cell concentration, i.e., the CaSki Cell-lines (epidermoid cervical carcinoma cells). Aiming to develop a non-clogging microconcentrator, we proposed a new counter-flow concentration unit characterized by the directions of penetrating flows being at an obtuse angle to the main flow, due to employment of streamlined turbine blade-like micropillars. Based on the optimization results of the counter-flow unit profile, a fractal arrangement for the counter-flow concentration unit was developed. A counter-flow microconcentrator chip was then designed and fabricated, with both the processing layer and collecting layer arranged in terms of the honeycomb structure. Visualized experiments using CaSki cell samples on the microconcentrator chip demonstrated that no cell-clogging phenomena occurred during the test and that no cells were found in the final filtrate. The test results show an excellent concentration performance for the microconcentrator chip, while a concentrating ratio of >4 with the flow rate being below 1.0 ml/min. As only geometrical structure is employed in the passive device, the counter-flow microconcentrator can be easily integrated into advanced microfluidic systems. Owing to the merit of non-clogging and continuous processing ability, the counter-flow microconcentrator is not only suitable for the sample preparation within biomedical field, but also applicable in water-particle separation.     

An empirical investigation of architectural prototyping

Architectural prototyping is the process of using executable code to investigate stakeholders’ software architecture concerns with respect to a system under development. Previous work has established this as a useful and cost-effective way of exploration and learning of the design space of a system and in addressing issues regarding quality attributes, architectural risks, and the problem of knowledge transfer and conformance. However, the actual industrial use of architectural prototyping has not been thoroughly researched so far. In this article, we report from three studies of architectural prototyping in practice. First, we report findings from an ethnographic study of practicing software architects. Secondly, we report from a focus group on architectural prototyping involving architects from four companies. And, thirdly, we report from a survey study of 20 practicing software architects and software developers. Our findings indicate that architectural prototyping plays an important and frequent role in resolving problems experimentally, but less so in exploring alternative solutions. Furthermore, architectural prototypes include end-user or business related functionality rather than purely architectural functionality. Based on these observations we provide recommendations for effective industrial architectural prototyping.     

On notions of regularity for data languages

With motivation from considerations in XML database theory and model checking, data strings have been introduced as an extension of finite alphabet strings which carry, at each position, a symbol and a data value from an infinite domain. Previous work has shown that it is difficult to come up with an expressive yet decidable automaton model for data languages. Recently, such a model, data automata, was introduced. This paper introduces a simpler but equivalent model and investigates its expressive power, algorithmic and closure properties, and some extensions.     

Design and implementation of the Node Identity Internetworking Architecture

The Internet Protocol (IP) has been proven very flexible, being able to accommodate all kinds of link technologies and supporting a broad range of applications. The basic principles of the original Internet architecture include end-to-end addressing, global routeability and a single namespace of IP addresses that unintentionally serves both as locators and host identifiers. The commercial success and widespread use of the Internet have lead to new requirements, which include Internetworking over business boundaries, mobility and multi-homing in an untrusted environment. Our approach to satisfy these new requirements is to introduce a new Internetworking layer, the node identity layer. Such a layer runs on top of the different versions of IP, but could also run directly on top of other kinds of network technologies, such as MPLS and 2G/3G PDP contexts. This approach enables connectivity across different communication technologies, supports mobility, multi-homing, and security from ground up. This paper describes the Node Identity Architecture in detail and discusses the experiences from implementing and running a prototype.     

Integrated micro Pirani gauge based hermetical package monitoring for uncooled VO x bolometer FPAs

This paper presents the design and fabrication of a micro Pirani gauge using VO _x as the sensitive material for monitoring the pressure inside a hermetical package for micro bolometer focal plane arrays (FPAs). The designed Pirani gauge working in heat dissipating mode was intentionally fabricated using standard MEMS processing which is highly compatible with the FPAs fabrication. The functional layer of the micro Pirani gauge is a VO _x thin film designed as a 100 × 200 μm pixel, suspended 2 μm above the substrate. By modeling of rarefied gas heat conduction using the Extended Fourier’s law, finite element analysis is used to investigate the sensitivity of the pressure gauge. Also the thermal interactions between the micro Pirani gauge and bolometer FPAs are verified. From the fabricated prototype, the measured device TCR is about −0.8% K⁻¹ and the sensitivity about 1.84 × 10⁻³ W K⁻¹ mbar⁻¹.     

A boundary value problem for the KdV equation: Comparison of finite-difference and Chebyshev methods

Solutions of a boundary value problem for the Korteweg–de Vries equation are approximated numerically using a finite-difference method, and a collocation method based on Chebyshev polynomials. The performance of the two methods is compared using exact solutions that are exponentially small at the boundaries. The Chebyshev method is found to be more efficient.     

Computer and Smartphone Continuance Intention: A Motivational Model

This paper develops and tests a motivational model to explain the overall continuance intention to use computers and smartphones. Based on survey data from 192 undergraduate students, structural equation modeling analysis is used to report: (1) the independent effect of intrinsic motivation on the continuance intention to use computers and smartphones; (2) the independent effect of extrinsic motivation on the continuance intention to use computers but not smartphones; (3) intrinsic motivation mediating the effect of extrinsic motivation on the continuance intention to use computers; (4) the independent effect of technology cognizance on the continuance intention to use computers but not smartphones; (5) intrinsic motivation positively influencing extrinsic motivation and technology cognizance, with respect to both devices. This research contributes to an improved understanding of the independent and interrelated effects of intrinsic and extrinsic motivations to use technological devices. The findings have important implications for theory and practice regarding the overall use of technology.