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151.
Hiroki Akasaka Aoi TakedaTsuneo Suzuki Masayuki NakanoShigeo Ohshio Hidetoshi Saitoh 《Diamond and Related Materials》2011,20(2):213-216
To develop hydrogenated amorphous carbon (a-C:H) as a biocompatible coating, a-C:H was studied in terms of its protein adsorption during the initial process of cell adsorption. A multilayer surface plasmon resonance (SPR) device consisting of an a-C:H layer on Au was built in the Kretschmann configuration to detect protein adsorption on an a-C:H film surface. From the dependence of reflectivity on the laser incident angle, SPR angle was determined to the incident angle in which the light intensity was reduced drastically. The proteins considered were lysozyme (Lyz) and fibrinogen (Fib). The SPR angle increased from 58.09 to 58.69° upon the adsorption of Lyz when the nonadsorbed Lyz was removed after introduction of 20 μM Lyz-containing solution. Upon the adsorption of Fib, the SPR angle increased from 60.95 to 61.76° when the nonadsorbed Fib were removed after the introduction of 0.4 μM Fib-containing solution. The shift in the SPR angle was small for both cases. Obtained results suggested that the number of adsorbed Lyz was higher than that of adsorbed Fib. 相似文献
152.
Taha Ahmad Iskandar Bin Haji Mohd Kimoto Takashi Kanada Teruyuki Okuyama Hidetoshi 《Food science and biotechnology》2013,22(1):53-58
High yield of cell, lipid, and docosahexaenoic acid (DHA) from thraustochytrid strain 12B were achieved without the use of a complex medium and at low NaCl concentration which is detrimental to avoid unnecessary corrosion of steel tank equipment during cultivation. Culture medium that contained only 0.1% NaCl and 1% MgSO4 in an organic base solution containing 8% glucose, 1% yeast extract, and 1% peptone, referred here as NM medium, was found to be as good as or superior to the culture medium prepared from 50%(v/v) seawater with percentage lipid/dry cell weight (DCW) of 66.4%(w/w) and DHA yield up to 43.95 mg/g DCW for the thraustochytrid strain 12B. The NM medium was also applicable to the prominently high DHA-accumulating Schizochytrium limacinum SR21, and therefore this medium could probably be used for other thraustochytrid and other types of microbial strains as well. 相似文献
153.
Weld Shape Variation and Electrode Oxidation Behavior under Ar-(Ar-CO_2) Double Shielded GTA Welding
Double shielded gas tungsten arc welding (GTAW, also known as tungsten inert gas (TIG) welding) of an SUS304 stainless steel with pure inert argon as the inner layer shielding and the Ar-CO2 or CO2 active gas as the out layer shielding was proposed in this study to investigate its effect on the tungsten electrode protection and the weld shape variation. The experimental results showed that the inner inert argon gas can successfully prevent the outer layer active gas from contacting and oxidizing the tungsten electrode during the welding process. Active gas, carbon dioxide, in the outer layer shielding is decomposed in the arc and dissolves in the liquid pool, which effectively adjusts the active element, oxygen, content in the weld metal. When the weld metal oxygen content is over 70×10-6, the surface-tension induced Marangoni convection changes from outward into inward, and the weld shape varies from a wide shallow one to a narrow deep one. The effect of the inner layer gas flow rate on the weld bead morphology and the weld shape was investigated systematically. The results show that when the flow rate of the inner argon shielding gas is too low, the weld bead is easily oxidized and the weld shape is wide and shallow. A heavy continuous oxide layer on the liquid pool is a barrier to the liquid pool movement. 相似文献
154.
Porous Titania Ceramic Prepared by Mimicking Silicified Wood 总被引:6,自引:0,他引:6
Toshitaka Ota Masaki Imaeda Haruyuki Takase Mikihiro Kobayashi Noboru Kinoshita Tunehisa Hirashita Hidetoshi Miyazaki Yasuo Hikichi 《Journal of the American Ceramic Society》2000,83(6):1521-1523
A porous titania ceramic with a woodlike microstructure that was analogous to silicified wood was prepared. The production of "titanified wood" was performed using the following process: (i) introduction of titanium tetraisopropoxide into wood materials via vacuum infiltration, (ii) hydrolysis of the titanium tetraisopropoxide in the cell structure to form a titania gel, and (iii) firing at a temperature of 600°–1400°C in air. The resulting titania ceramic had the same external and internal forms of the original wood. 相似文献
155.
Facile purification of tocopherols from soybean oil deodorizer distillate in high yield using lipase 总被引:16,自引:0,他引:16
Yuji Shimada Seiichi Nakai Masaharu Suenaga Akio Sugihara Motohiro Kitano Yoshio Tominaga 《Journal of the American Oil Chemists' Society》2000,77(10):1009-1013
Tocopherols have been purified from deodorizer distillate produced in the final deodorization step of vegetable oil refining
by a process including molecular distillation. Deodorizer distillate contains mainly tocopherols, sterols, and free fatty
acids (FFA); the presence of sterols hinders tocopherol purification in good yield. We found that Candida rugosa lipase recognized sterols as substrates but not tocopherols, and that esterification of sterols with FFA could be effected
with negligible influence of water content. Enzymatic esterification of sterols with FFA was thus used as a step in tocopherol
purification. High boiling point substances including steryl esters were removed from soybean oil deodorizer distillate by
distillation, and the resulting distillate (soybean oil deodorizer distillate tocopherol concentrate; SODDTC) was used as
a starting material for tocopherol purification. Several factors affecting esterification of sterols were investigated, and
the reaction conditions were determined as follows: A mixture of SODDTC and water (4∶1, w/w) was stirred at 35°C for 24 h
with 200 U of Candida lipase per 1 g of the reaction mixture. Under these conditions, approximately 80% of sterols was esterified, but tocopherols
were not esterified. After the reaction, tocopherols and FFA were recovered as a distillate by molecular distillation of the
oil layer. To enhance further removal of the remaining sterols, the lipase-catalyzed reaction was repeated on the distillate
under the same reaction conditions. As a result, more than 95% of the sterols was esterified in total. The resulting reaction
mixture was fractionated to four distillates and one residue. The main distillate fraction contained 65 wt% tocopherols with
low contents of FFA and sterols. In addition, the residue fraction contained high-purity steryl esters. Because the process
presented in this study includes only organic solvent-free enzymatic reaction and molecular distillation, it is feasible as
a new industrial purification method of tocopherols.
This work was presented at the Biocatalysis symposium in April 2000, held at the 91st Annual Meeting and Expo of the American
Oil Chemists Society, San Diego, CA. 相似文献
156.
Kenichi OKADA Hidetoshi ONODERA Keikichi TAMARU 《Analog Integrated Circuits and Signal Processing》2000,25(3):309-318
Layout has strong influence on matching properties of a circuit. Current matching models, which characterize both local random non-uniformities and global systematic non-uniformities stochastically, are not adequate for the matching analysis taking the effect of layout realization into account. In order to consider topological information of layout into matching analysis, we propose a matching model which treats the random and systematic components separately. Also, we characterize the micro-loading effect, which modulates fabricated line-width according to the local density of layout patterns, into matching analysis. With these two techniques, we can perform matching analysis of CMOS circuits taking layout information into account. 相似文献
157.
The multi-axial warp knitted fabric (MWK) is a useful reinforcement for composite. Higher mechanical performance resulted from no crimp of the fibre bundle is achieved compared with the general textile composites. For the fibre bundle of MWK, only one type of reinforcement fibre among glass, carbon fibre bundle, and so on has been selected. The mechanical properties and the cost of MWK composite depend on the feature of the fibre bundle. In order to extend the applicability of composite, the concept of “fibre hybrid composite” was applied into the MWK composite. Two kinds of fibre bundle; carbon and glass, were used in the 0/90 multi-axial warp knitted fabric. As the fibre hybrid composite; the inter-layer hybrid composite in which each layer was fabricated by carbon and glass fibre bundle respectively was investigated. Impact properties of composite were investigated by using drop weight impact tests. In case of unsaturated polyester, total energy and progressive energy of inter-layer fibre hybrid composite realized the highest value in all specimens. However, in case of epoxy resin, inter-layer hybrid composite didn’t realize the highest value in all specimens. The difference in energy absorption capability could be described with the fracture mechanism. 相似文献
158.
159.
Shiomi N Yamaguchi Y Nakai H Fujita T Katsuda T Katoh S 《Journal of Bioscience and Bioengineering》2006,102(3):206-209
The rates of degradation of cyanuric acid, a key intermediate in a metabolic pathway of s-triazine herbicides, were measured for Pseudomonas sp. NRRL B-12227. The rate of degradation was affected by the rate of cyanuric acid transport through cell membranes and the activity of cyanuric acid amidohydrolase inside the cells. At low concentrations of cyanuric acid, the acclimation of cells to cyanuric acid and/or added nutrients effectively enhanced the degradation rate. The strain was also applied to bioremediation using a Bioremediation with Self-Immobilization System (BSIS), in which Pseudomonas sp. NRRL B-12227 cells were co-immobilized with Bacillus subtilis, the latter of which secretes a viscous polymer, in a shallow layer of soil packed in a column. More than 70% of the Pseudomonas sp. NRRL B-12227 cells were co-immobilized with the B. subtilis in a 7.5 cm layer of the packed soil by self-aggregation. More than 60% of the 1 mM cyanuric acid supplied to the packed soil was degraded in this layer during a 72 h period. 相似文献
160.
Riku Kawasaki Kosuke Kondo Risako Miura Keita Yamana Hinata Isozaki Risako Shimada Shogo Kawamura Hidetoshi Hirano Tomoki Nishimura Naoki Tarutani Kiyofumi Katagiri Alexandra Stubelius Shin-ichi Sawada Yoshihiro Sasaki Kazunari Akiyoshi Atsushi Ikeda 《International journal of molecular sciences》2022,23(9)
Developing photoactivatable theranostic platforms with integrated functionalities of biocompatibility, targeting, imaging contrast, and therapy is a promising approach for cancer diagnosis and therapy. Here, we report a theranostic agent based on a hybrid nanoparticle comprising fullerene nanocrystals and gold nanoparticles (FGNPs) for photoacoustic imaging and photothermal therapy. Compared to gold nanoparticles and fullerene crystals, FGNPs exhibited stronger photoacoustic signals and photothermal heating characteristics by irradiating light with an optimal wavelength. Our studies demonstrated that FGNPs could kill cancer cells due to their photothermal heating characteristics in vitro. Moreover, FGNPs that are accumulated in tumor tissue via the enhanced permeation and retention effect can visualize tumor tissue due to their photoacoustic signal in tumor xenograft model mice. The theranostic agent with FGNPs shows promise for cancer therapy. 相似文献