Effect of iodine doping of phthalocyanine on the photocurrent generation in a phthalocyanine/C60 heterojunction

Photocurrent generation in an indium-tin oxide (ITO)/iodine-doped Ni-phthalocyanine (NiPc-I_x)/C₆₀/In/Al heterojunction device with x ~ 1 was studied. By keeping the device in air after preparation, the device slowly reached a stationary state in which the sign of the photocurrent is opposite to that of a non-doped ITO/NiPc/C₆₀/In/Al device although the rectification direction for the dark current is the same. By a simulation of incident photon-to-current conversion efficiency spectra and a measurement of internal electric field by electroabsorption spectroscopy, it was elucidated that, in the doped device, the band bending near the phthalocyanine/C₆₀ interface is absent and the photocurrent is generated by a weak Schottky barrier at the C₆₀/In interface. It is also shown that the C₆₀ film encapsulates the doped iodine into the NiPc-I_x layer to stabilize the doping level and prevent the reaction of iodine with In.     

Automated fault tree synthesis by semantic network modeling, rulebased development and recursive 3-value procedure

A concept of flow is introduced to represent any material, information, energy, activity, or phenomenon which can move or propagate along flow paths to cause events specific to the system to be analyzed. A graphical equipment library is given to represent typical types of ‘generation rate’ and ‘aperture’ controllers. The system is modeled by a semantic network with labeled arrows showing effect to cause (backward) relationships between flow and equipment nodes. A correspondence between the equipment library and the system components is established, and the semantic network is constructed by integrating network fragments in the library. Fixed and/or free boundary conditions can be specified explicitly for flow or equipment nodes. Forward-chaining event development rules locally trace the labeled arrows, while a 3-value procedure guides the FT generation by recursive rule applications. The rules are obtained from tables and equipment definitions. The 3-value logic is used to truncate FTs according to the boundary conditions. Different FTs are generated for different top events and boundary conditions, given a semantic network model. FT modules and their hierarchies can be identified by examining network theoretic properties of flow nodes. The proposed approach is demonstrated for a relay system, a hypothetical swimming pool reactor and a chemical reactor.     

Comprehensive approach to structural and functional glycomics based on chemoselective glycoblotting and sequential tag conversion

Changes in protein glycosylation profoundly affect protein function. To understand these effects of altered protein glycosylation, we urgently need high-throughput technologies to analyze glycan expression and glycan-protein interactions. Methods are not available for amplification of glycans; therefore, highly efficient sample preparation is a major issue. Here we present a novel strategy that allows flexible and sequential incorporation of various functional tags into oligosaccharides derived from biological samples in a practical manner. When combined with a chemoselective glycoblotting platform, our analysis enables us to complete sample preparation (from serum to released, purified, methyl-esterified, and labeled glycans) in 8 h from multiple serum samples (up to 96 samples) using a 96-well microplate format and a standard de-N-glycosylation protocol that requires reductive alkylation and tryptic digestion prior to PNGase F digestion to ensure maximal de-N-glycosylation efficiency. Using this technique, we quantitatively detected more than 120 glycans on human carcinoembryonic antigens for the first time. This approach was further developed to include a streamlined method of purification, chromatographic fractionation, and immobilization onto a solid support for interaction analysis. Since our approach enables rapid, flexible, and highly efficient tag conversion, it will contribute greatly to a variety of glycomic studies.     

Systemic and mucosal immune responses of mice to aluminium-adsorbed or aluminium-non-adsorbed tetanus toxoid administered intranasally with recombinant cholera toxin B subunit

For the purpose of changing the immunization procedure of tetanus toxoid from intramuscular or subcutaneous injection, which has been in practice for a long time, to intranasal administration, we examined systemic and mucosal immune responses of mice to aluminium-adsorbed tetanus toxoid (aTT) and aluminium-non-adsorbed tetanus toxoid (nTT) inoculated intranasally with recombinant cholera toxin B subunit (rCTB). Intranasal immunization with aTT induced, at a concentration of 0.5 Lf, high levels of TT-specific serum IgG antibody titres and moderate levels of TT-specific serum IgA antibody titres in the presence and absence of rCTB. Induction of high or moderate levels of mucosal TT-specific IgA antibody responses was observed with and without rCTB in the lung, the nasal cavity, the small and large intestines and the vagina. Generally speaking, the co-administration of aTT and rCTB showed higher mucosal TT-specific IgA antibody titres when compared with the administration of aTT alone. In case of intranasal administration of nTT, the dose of 5 Lf was necessary and stimulated, only in the presence of rCTB (10 micrograms), high levels of tetanus toxoid (TT)-specific serum IgG antibody responses in all mice examined and moderate or slight levels of TT-specific IgA antibody responses in the nasal, pulmonary and small and large intestinal lavages of a few mice. All mice intranasally immunized with aTT alone or nTT and rCTB escaped onset of tetanus. This is the first report concerned with the mucosal adjuvant activity of an aluminium compound. Judging from these results, intranasal administration of aTT with and without rCTB or nTT with rCTB appears to be a very useful means for a vaccination against tetanus with respect to ease, safety, certainty, low cost and no need for an injection needle.     

Lung cancer: intermittent irradiation synchronized with respiratory motion--results of a pilot study

Pyothorax-associated lymphoma (PAL) is a newly-described entity developing several decades after artificial pneumothorax treatment for pulmonary or pleural tuberculosis. It is known to be associated with Epstein-Barr virus (EBV) with constant expression of the two latent membrane proteins: latent membrane protein (LMP)-1 and EBV-associated nuclear antigen (EBNA)-2. We are reporting three new cases of PAL. All of the tumours were of B-cell lineage and classified as large-cell diffuse lymphomas according to the International Working Formulation for the Classification of Lymphomas. The EBV genome was detected in two of the cases with LMP-1 and EBNA-2 expression. No EBV could be detected in the third case suggesting that different mechanisms may be involved in the pathogenesis of the disease. Body cavity-based high grade lymphomas (BCBL) represent a new disease, developing mainly in human immunodeficiency virus (HIV) infected patients: the tumoural cells often contain both human herpes virus (HHV)-8 (or Kaposi's sarcoma herpes virus) and EBV genomes, suggesting that these viruses might co-operate in the pathogenesis of the disease. The pleural location and the association of EBV have led to speculation that PAL could also be related to HHV-8 infection. However, no HHV-8 genome could be detected in any of the 14 tested cases already reported in the literature nor in the two cases we studied (one EBV-positive and one EBV-negative), suggesting that PAL and BCBL are two different entities.     

Metastasis of hepatocellular carcinoma to the colon demonstrated by Tc-99m PMT scintigraphy

A 57-year-old man was found to have a tumor in the ascending colon after hepatic segmentectomy for ruptured hepatocellular carcinoma. Colonoscopy was performed, and metastasis of the hepatocellular carcinoma to the ascending colon was suspected based on the biopsy specimens obtained from the tumor. On hepatobiliary scintigraphy using Tc-99m PMT performed to confirm this diagnosis, Tc-99m PMT was clearly observed to accumulate in the tumor. Thus, the tumor was diagnosed as metastasis of hepatocellular carcinoma.     

Formation of a negative impulse discharge induced by an XeCl excimer laser in a long rod‐to‐rod electrode gap in air

Negative discharge induction experiments were performed with a rod‐to‐rod electrode configuration having a gap length of 0.8 m using a high‐power XeCl excimer laser of maximum output energy of 2 J/pulse and an impulse generator of maximum voltage of 1.2 MV. Development of a leader in the laser‐ionized plasma channel was observed by using an image converter camera. When an XeCl excimer laser is applied at a carefully controlled instant, the guiding effect for an impulse discharge is optimized. Three main results were obtained. First, the discharge started near the focal point and then developed bidirectionally toward the high‐voltage electrode and the grounded electrode. Midgap leaders were frequently observed. Second, it was observed that the lifetime of the laser‐ionized plasma channel was about 1 μs. Third, the discharge processes were classified into six stages. The mechanism of the streamer and leader propagation is discussed. © 2000 Scripta Technica, Electr Eng Jpn, 134(2): 11–18, 2001     

Study concerning production of lightning overvoltages on low‐voltage power distribution lines

In order to study lightning problems of low‐voltage power distribution lines, lightning overvoltage waveforms were observed inside the homes of customers. The cause of lightning overvoltages was examined from observation of striking points by still cameras. Lightning overvoltages of 62 waveforms were recorded by observation over a period of about 3 1/2 years. Observed waveforms can be classified into three types of single polarity (positive or negative), both polarities (which change from positive to negative or negative to positive), and pulsive waveform. The causes of these lightning overvoltages which were estimated from striking points are shown as follows: (1) Induced lightning overvoltages on low‐voltage distribution lines. (2)   Electric potential rise due to discharge of surge arresters or current of overhead ground wire. (3)   Shift of lightning overvoltages from high‐voltage side of transformer to low‐voltage side, which is due to electromagnetic induction. © 2000 Scripta Technica, Electr Eng Jpn, 130(4): 66–75, 2000     

Serum Autotaxin is not a Useful Biomarker for Ovarian Cancer

Autotaxin (ATX) is a glycoprotein that was first identified in the conditioned medium of human melanoma cells as an autocrine motility factor. It possesses lysophospholipase D activity, producing the bioactive lipid mediator lysophosphatidic acid (LPA) from lysophosphatidylcholine. Enhanced expression of ATX mRNA has been reported in various cancer cells and tissues, and it has been speculated that ATX overexpression in cancer cells may be associated with aberrant LPA production. LPA and ATX have been implicated in cancer progression and metastasis, and ovarian cancer is a representative example. In the present study, we measured the serum ATX antigen levels in patients with ovarian cancer and evaluated the usefulness of this parameter for clinical laboratory testing. The serum ATX antigen levels were not increased in ovarian cancer patients as compared with the levels in healthy subjects, and the serum ATX may not be useful as a biomarker for ovarian cancer.