Unravel four hairpins!

DNA machines consisting of consecutive hairpins, which we have previously described, have various potential applications in DNA computation. In the present study, a 288-base DNA machine containing four consecutive hairpins was successfully constructed by ligation and PCR. PAGE and fluorescence spectroscopy experiments verified that all four hairpins were successfully opened by four opener oligomers, and that hairpin opening was dependent on the proper openers added in the correct order. Quantitative analysis of the final results by fluorescence spectroscopy indicated that all four hairpins were open in about 1/4 to 1/3 of the DNA machines.     

Technique for anisotropic extension of organic crystals: application to temperature dependence of electrical resistance

We have developed a technique for the anisotropic extension of fragile molecular crystals. The pressure medium and the instrument, which extends the pressure medium, are both made from epoxy resin. Since the thermal contraction of our instrument is identical to that of the pressure medium, the strain applied to the pressure medium has no temperature dependence down to 2 K. Therefore, the degree of extension applied to the single crystal at low temperatures is uniquely determined from the degree of extension in the pressure medium and thermal contractions of the epoxy resin and the single crystal at ambient pressure. Using this novel instrument, we have measured the temperature dependence of the electrical resistance of metallic, superconducting, and insulating materials. The experimental results are discussed from the viewpoint of the extension (compression) of the lattice constants along the parallel (perpendicular) direction.     

A Face Identification System Based on the Mahalanobis–Taguchi System

In this paper, we propose a face identification system based on the Mahalanobis–Taguchi System (MTS). The MTS is one of the pattern recognition methods frequently used in quality engineering, and can perform robust pattern recognition by using training data, including noise. It is likely that this advantage will allow the effective implementation of a robust face identification system against lighting and face position fluctuations. Moreover, the MTS can optimize the number of attributes required for identification by using the orthogonal array and the signal/noise (SN) ratio. The face identification system has to deal with many users and the amount of data in a facial image is large. Therefore, the time required for identification can be decreased and the amount of data in the facial image database can be reduced by performing the optimization. We confirmed the effectiveness of the proposed system through practical experiments. The experimental results revealed that the MTS was an effective method for robust face identification, and could effectively reduce the number of attributes required for identification.     

Site-specific electronic structure analysis by channeling EELS and first-principles calculations

Site-specific electronic structures were investigated by electron energy loss spectroscopy (EELS) under electron channeling conditions. The Al-K and Mn-L(2,3) electron energy loss near-edge structure (ELNES) of, respectively, NiAl2O4 and Mn3O4 were measured. Deconvolution of the raw spectra with the instrumental resolution function restored the blunt and hidden fine features, which allowed us to interpret the experimental spectral features by comparing with theoretical spectra obtained by first-principles calculations. The present method successfully revealed the electronic structures specific to the differently coordinated cationic sites.     

Generating Instances for MAX2SAT with Optimal Solutions

A test instance generator (an instance generator for short) for MAX2SAT is a procedure that produces, given a number n of variables, a 2-CNF formula F of n variables (randomly chosen from some reasonably large domain), and simultaneously provides one of the optimal solutions for F. We propose an outline to design an instance generator using an expanding graph of a certain type, called here an "exact 1/2-enlarger". We first show a simple algorithm for constructing an exact 1/2-enlarger, thereby deriving one concrete polynomial-time instance generator GEN. We also show that an exact 1/2-enlarger can be obtained with high probability from graphs randomly constructed. From this fact, we propose another type of instance generator RGEN; it produces a 2-CNF formula with a solution which is optimal for the formula with high probability. However, RGEN produces less structured formulas and a much larger class of formulas than GEN. In fact, we prove the NP-hardness of MAX2SAT over the set of 2-CNF formulas produced by RGEN.     

Autonomic control of heart rate during physical exercise and fractal dimension of heart rate variability

The objectives of the present study were to investigate autonomic nervous system influence on heart rate during physical exercise and to examine the relationship between the fractal component in heart rate variability (HRV) and the system's response. Ten subjects performed incremental exercise on a cycle ergometer, consisting of a 5-min warm-up period followed by a ramp protocol, with work rate increasing at a rate of 2.0 W/min until exhaustion. During exercise, alveolar gas exchange, plasma norepinephrine (NE) and epinephrine (E) responses, and beat-to-beat HRV were monitored. HRV data were analyzed by "coarse-graining spectral analysis" (Y. Yamamoto and R. L. Hughson. J. Appl. Physiol. 71: 1143-1150, 1991) to break down their total power (Pt) into harmonic and nonharmonic (fractal) components. The harmonic component was further divided into low-frequency (0.0-0.15 Hz) and high-frequency (0.15-0.8 Hz) components, from which low-frequency and high-frequency power (Pl and Ph, respectively) were calculated. Parasympathetic (PNS) and sympathetic (SNS) nervous system activity indicators were evaluated by Ph/Pt and Pl/Ph, respectively. From the fractal component, the fractal dimension (DF) and the spectral exponent (beta) were calculated. The PNS indicator decreased significantly (P < 0.05) when exercise intensity exceeded 50% of peak oxygen uptake (VO2 peak). Conversely, the SNS indicator initially increased at 50-60% VO2peak (P < 0.05) and further increased significantly (P < 0.05) at > 60% VO2peak when there were also more pronounced increases in NE and E.(ABSTRACT TRUNCATED AT 250 WORDS)     

Chemical and electrochemical preparation of poly(9,10-dihydrophenanthrene-2,7-diyl) using nickel complexes and electrochemical properties of the polymer

Summary Dehalogenation polycondensations of 2,7-dibromo-9,10-dihydrophenanthrene with isolated zero-valent nickel complex and electrochemically generated zero-valent nickel complex afford -conjugated poly(9,10-dihydrophenanthrene-2,7-diyl). The electrochemically synthesized polymer is obtained as a thin film on electrode, and shows a reversible electrochemically doping-undoping cycle in an oxidation region. The polymer has essentially the same -conjugation system as that of poly(p-phenylene).     

Suppression of sidelobe levels for guided-wave acousto-optic tunable filters using weighted coupling

An analysis of guided-wave acousto-optic tunable filters (AOTFs) that employ simple acousto-optic (AO) weighted coupling techniques for sidelobe reduction and the calculated and experimental results from a specific example that involves only variation of the width of a surface acoustic wave (SAW) slot waveguide are presented. The calculations on single- and multi-stage AOTFs consisting of an optical channel waveguide and a SAW slot waveguide in LiNbO(3) substrate show that waveguide width weighting using generalized Hamming functions would provide significant improvement in sidelobe suppression. Calculated results together with the design, fabrication, and measured performance characteristics of a single-stage AOTF that utilizes a weighted-aperture SAW slot waveguide in YX-LiNbO(3) substrate at the optical wavelength of 1.55 mum and the acoustic center frequency of 175 MHz are reported. The measured sidelobe level is -13.6 dB and the measured FWHM bandwidth is 26 A, as compared to the theoretical values of -15.0 dB and 15 A, respectively. The RF drive power was measured to be 1.0 W at a mode-conversion efficiency of 100%     

New oxide phosphors for FEDs: Ruddlesden—Popper phases synthesized with IIIB‐group elements

Abstract— A red‐emitting phosphor, SrTiO³:Pr³⁺, for low‐voltage‐type FEDs and VFDs was developed by Futaba Corporation in 1996. The addition of Al or Ga is essential in the preparation of this phosphor because it improves the luminescence efficiency dramatically. For this impurity effect, Futaba Corporation proposed a charge‐compensation mechanism, which was supported by a recent observation of emission lines due to Al³⁺‐Pr³⁺ pairs. In addition, it was found that Al also works as a scavenger of planar defects, presumably SrO thin layers interleaved in the SrTiO³ lattice, by forming strontium aluminates. The latter mechanism suggests the possibility that a similar impurity effect can be found in materials with crystal structures, including alkaline‐earth oxide layers (Ruddlesden‐Popper phases).