组态王在火电机组热经济性分析系统中的应用

针对大型热力发电厂性能分析系统可移植性较差的现状,介绍了一个新型的通用性强的在线火电机组热经济性分析系统。本系统采用组态王组态软件设计。简述了组态过程,给出了软件结构,将热耗计算程序挂接到该组态软件中,运行良好,结果正确。     

超声电化学沉积ZnO薄膜及其机理研究

采用超声电化学沉积生长了c轴取向的ZnO薄膜,并与常规电化学沉积生长的ZnO薄膜进行了比较.采用X射线衍射(XRD)、扫描电子显微镜(SEM)、紫外-可见分光光度计研究了样品的晶体结构、表面形貌以及光学性能.结果表明,常规电化学沉积生长的ZnO薄膜为纳米柱状结构,随着沉积电压的增加,c轴取向率呈极值变化,热退火可提高这种薄膜的透射率.超声电化学沉积生长的ZnO薄膜,在1.5V低沉积电压下仍为柱状结构,但比未加超声电化学法沉积的样品具有更好的c轴取向,沉积电压增加到2.0V时样品则变为麦粒状结构,热退火后其透射率几乎不变.     

CD59活性位点相关性基因突变的构建与表达

构建了野生型和突变型CD59重组质粒,建立了高效真核表达系统,探讨了W40位点的生物学活性。采用基因点突变技术使CD59W40基因位置缺失（突变1,M1）及C39W40K41→W39W40W41（突变2,M2）,重叠延伸PCR（overlap extension PCR）定点诱变扩增突变基因,重组入真核表达质粒pIRES,利用阳离子脂质体（Lipfectamine2000）将重组质粒转染中国仓鼠卵巢细胞（CHO）进行表达。酶切鉴定及序列测定证实成功构建了pIRES-MICD59、pIRES—M2CD59和pIRES-WTCD59,突变基因约500bp。G418筛选出了CHO转染细胞的稳定细胞克隆,免疫荧光、ELISA检测筛选MICD59、M2CD59和WTCD59蛋白高表达株,连续传代30代有高表达;补体溶细胞反应显示与野生型CD59相比,突变型M1CD59失去对补体的抑制功能,而M2CD59抗补体活性略增高。证实CD59的W40位点对其功能具有重要作用,封闭此位点可提高补体活性,有望用于肿瘤治疗。     

曝气生物滤池去除受污染饮用水源水中的氨氮

采用曝气生物滤池(BAF)对受污染水源水进行了除氨氮效果研究,结果表明,当原水氨氮质量浓度在0.240～1.407 mg/L范围内时,氨氮平均去除率为83%,进水氨氮的质量浓度人工增加至O.717～12.455 mg/L时,氨氮平均去除率为88%,具有较好的氨氮去除效率和抗冲击负荷能力;同时探讨了进水氨氮容积负荷对氨氮去除效果的影响.     

Molecular Identification of the G-Protein Genes and Their Expression Profiles in Response to Nitrogen Deprivation in Brassica napus

Heterotrimeric guanine nucleotide binding protein (G-protein) consisting of Gα, Gβ, and Gγ subunits is one of the key signal transducers in plants. Recent studies indicated that G-protein has been proposed as an important mediator of nitrogen responses in rice, wheat, and Arabidopsis. However, little is known about these G-proteins in Brassica napus (B. napus), except for three identified G-proteins, BnGA1, BnGB1, and BnGG2. Therefore, the aim of the present study is to characterize the members of the G-protein gene family in allotetraploid B. napus and to analyze their expression profiles in response to nitrogen deprivation. In total, 21 G-protein family members were identified in B. napus, encoding two Gα, six Gβ, and 13 Gγ. Sequence and phylogenetic analyses showed that although genome-wide triploid events increased the number of genes encoding Gα, Gβ, and Gγ subunits, the gene structure and protein properties of the genes encoding each G-protein subunit were extremely conserved. Collinearity analysis showed that most G-protein genes in B. napus had syntenic relationships with G-protein members of Arabidopsis, Brassica rape (B. rapa), and Brassica oleracea (B. oleracea). Expression profile analysis indicated that Gα and C-type Gγ genes (except BnGG10 and BnGG12 were highly expressed in flower and ovule) were barely expressed in most organs, whereas most Gβ and A-type Gγ genes tended to be highly expressed in most organs. G-protein genes also showed various expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, Gα and five C-type Gγ genes were upregulated initially in roots, while in leaves, Gα was downregulated initially and five C-type Gγ genes were highly expressed in different times. These results provide a complex genetic dissection of G-protein genes in B. napus, and insight into the biological functions of G-protein genes in response to nitrogen deficiency.     

MEMS微型双稳态电磁继电器的设计

本文利用通电平面螺旋线圈产生磁场特点,采用镜像绕制以及并联连接双线圈的方法,研制了一种快速低功耗MEMS微型双稳态继电器结构,其尺寸约为3mm*4.5mm*1.5mm,文中着重分析其工作原理和过程.经过分析,该继电器在通电电流80～120mA时,开关时间约在0.1ms～0.2ms之间,因此,其具有体积小、开关速度快、低功耗等优点,并且易于集成和阵列,适用于能源有限并且可靠性要求高的微型航天器以及便携设备中.     

某型柴油机增压系统概述

增压技术能提高柴油机功率和改善经济性能,某型柴油机增压技术非常先进,是该公司核心技术之一。本文分析了其增压系统的特点,并介绍了增压器的特殊功能和先进结构。     

隔河岩枢纽泄洪消能试验研究

本文着重介绍隔河岩枢纽泄洪消能试验研究成果。通过试验,将表孔、深孔、底孔采取平面错开,空间分层,出口采用收缩射流转向的新技术,既满足了泄洪要求,又有较好的消能防冲效果。上述研究成果为设计,施工提供了大量的数据,解决了工程中许多重大的技术难题。     

预应力锚索在堆积体内施工技术的难点与对策

根据堆积体不同组成,选取偏心和同心跟管工艺保证成孔效率,实现快速施工,迅速稳定边坡。     

有横缝高拱坝的动力接触模型及其数值解

本文提出了地震作用下有横缝高拱坝的动接触模型，并对这一强非线性模型进行数值计算。该动态接触模型是基于有伸缩横缝拱坝在地震作用下其响应所呈现的非线性摩擦接触特性，应用接触力学分析方法建立的，能够充分地反映高拱坝横缝的张开 闭合 摩擦力学过程的分析模型。结果表明，采用本模型的计算效率较高、收敛性有保证，结果合理、可靠。