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11.
Tryptic, thermitatic, and tryptic-thermitatic Faba bean protein hydrolyzates as well as their equimolar mixture of amino acids were perfused through proximal and distal parts of the intestine (10 cm length) of non-narcotized rats. The total amino-acid concentration of the perfused solution was 50 mM. The absorption of nitrogen and total amino acids from the tryptic and tryptic-thermitatic hydrolyzates was lower than that from the amino-acid mixture, the absorption from the thermitatic hydrolyzate was in accordance with that from the amino-acid mixture. The absorption pattern of the amino acids which preferably undergo a peptidic absorption is similar with the three hydrolyzates: in the proximal intestinal part this concerns glutamic acid and serine, in the distal intestinal part--methionine, alanine, glycin, and serine. The absorption pattern of the amino acids is different between the three hydrolyzates and the amino-acid mixture. Between the absorption pattern of the amino acids from the three hydrolyzates little differences were evident only in the proximal intestinal part. The coefficients of variation of the tryptic-thermitatic hydrolyzates are in accordance with those of the amino-acid mixture, whereas that of the thermitatic hydrolyzates is significantly lower. In the distal intestinal part all supplied forms are more rapidly absorbed than in the proximal part of the intestine. 相似文献
12.
Since the end of the Cold War and due to the needs to produce titanium more cost‐efficient and thus more attractive for its civilian use, the electroslag remelting (ESR) process has become an increasingly important topic of international research programmes, because titanium and titanium aluminides can be chemically refined by ESR in some degree. Using ESR, titanium turnings from machining steps and scrap from foundries can be remelted, refined and provided as secondary titanium for the market at relatively favourable prices. This article investigates the removability of the main impurities out of titanium and titanium‐aluminium alloys by electroslag remelting using the active slag system CaF2‐Ca‐(CaO). Thermochemical and kinetic aspects of the ESR process are considered. 相似文献
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Helmut Richter Dr. rer. nat. Horst W. Tamler Dr. rer. nat. 《JOM Journal of the Minerals, Metals and Materials Society》1994,46(10):46-47
A new on-line texture-analyzing system and its application to nondestructive r value determination is discussed. In addition to providing a brief theoretical background and describing the instrumental set-up, the article presents off-line measurements with this equipment and demonstrates the high accuracy of the determined r-values. A special feature of the unit is the possibility to simultaneously measure the most important r values—r0, r45, r90, and rm. 相似文献
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The main objective of this fundamental study was to investigate effects of processing conditions and resulting matrix morphology on interfacial bond strength of fibre reinforced thermoplastic composites. Using a hot stage microscope, single fibre pull-out samples were produced with T700S high strength carbon fibre and two semicrystalline thermoplastic matrices, polyphenylene sulphide (PPS) and polyethylene terephthalate (PET), respectively. Processing temperatures and cooling histories were the major variables in sample preparation. The T700S fibre had no clear effect on the surrounding PPS and PET matrix morphology, as long as direct cooling at constant rates was selected. A transcrystalline phase around the fibres could be induced in the T700S/PPS system, if isothermal crystallization was carried out at 227°C. Fibre pull-out tests were conducted at room temperature and two basic failure paths were observed, i.e. debonding at the fibre-matrix interface and cohesive failure of the matrix close to the fibre surface. The results indicate that slow cooling rate and a resulting coarse spherulitic morphology around the fibres correlate with high interfacial shear strength. In fact somewhat higher strength values were obtained for samples with transcrystalline layers around the fibres. 相似文献
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Hartmut Rehbein Horst Karl 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1985,180(5):373-378
Summary The extraction of fish muscle protein using SDS containing solubilization buffers was studied varying the time and the temperature of solubilization, as well as pH and SDS concentration of the buffer. At pH < 6 the myofbrillar proteins were incompletely solubilized; temperatures of 80-100 °C resulted in protein degradation observable in the SDS-PAGE.Samples of fish muscle containing high amounts of formaldehyde (50 mmoles FA/kg wet weight) could only be solubilised at 100 °C; on the other hand it was possible to solubilize cooked and/or canned products under mild conditions (2% SDS, 1% 2-ME, pH 8.9, shaking for 2 h at 60 °C).
Abbreviations FA formaldehyde - IEF isoelectric focusing - 2-ME 2-mercaptoethanol - PA polyacrylamide - PAGE polyacrylamide gel electrophoresis - pI isoelectric point - SDS sodium dodecyl sulfate - TMAO trimethylamine oxide - Tris Tris (hydroxymethyl)methylamine 相似文献
Solubilisierung von Fischmuskelproteinen durch Natriumdodecylsulfathaltige Puffer
Zusammenfassung Der Einfluß von Solubilisierungszeit und -temperatur, des pH-Wertes und der SDS-Konzentration des Solubilisierungspuffers auf die Extraktion von Fischmuskelproteinen wurde überprüft. Bei pH-Werten < 6 wurden die myofbrillären Proteine nur unvollständig gelöst; Solubilisierungstemperaturen von 80 bis 100 °C führten zu einem in der SDS-PAGE sichtbaren Proteinabbau. Fischmuskelproben mit hohem Formaldehydgehalt (50 mmole FA/kg Feuchtgewicht) ließen sich nur bei 100 °C vollständig solubilisieren; demgegenüber gelang die weitgehende Solubilisierung gegarter und/oder sterilisierter Produkte unter milden Bedingungen (2% SDS, 1% 2-ME, pH 8,9; 2stündiges Schütteln bei 60 °C).
Abbreviations FA formaldehyde - IEF isoelectric focusing - 2-ME 2-mercaptoethanol - PA polyacrylamide - PAGE polyacrylamide gel electrophoresis - pI isoelectric point - SDS sodium dodecyl sulfate - TMAO trimethylamine oxide - Tris Tris (hydroxymethyl)methylamine 相似文献