A Universal Platform for Macromolecular Deliveryinto Cells Using Gold Nanoparticle Layers via the Photoporation Effect

Although promising, it is challenging to develop a simple and universal method for the high‐efficiency delivery of biomacromolecules into diverse cells. Here, a universal delivery platform based on gold nanoparticle layer (GNPL) surfaces is proposed. Upon laser irradiation, GNPL surfaces show such good photothermal properties that absorption of the laser energy causes a rapid increase in surface temperature, leading to enhanced membrane permeability of the cultured cells and the diffusion of macromolecules into the cytosol from the surrounding medium. The high‐efficiency delivery of different macromolecules such as dextran and plasmid DNA into different cell types is achieved, including hard‐to‐transfect mouse embryonic fibroblasts (mEFs) and human umbilical vein endothelial cells (HUVECs), while cell viability is well maintained under optimized irradiation conditions. The platform vastly outperforms the leading commercial reagent, Lipofectamine 2000, especially in transfecting hard‐to‐transfect cell lines (plasmid transfection efficiency: ≈53% vs ≈19% for mEFs and ≈44% vs ≈8% for HUVECs). Importantly, as the gold nanoparticles (GNPs) constituting the GNPL are firmly immobilized together, the potential cytotoxicity caused by endocytosis of GNPs is effectively avoided. This platform is reliable, efficient, and cost‐effective with high‐throughput and broad applicability across different cell types, opening up an innovative avenue for high‐efficiency intracellular delivery.     

Quality of Protection in Cloud-Assisted Cognitive Machine-to-Machine Communications for Industrial Systems

Cloud-assisted cognitive machine-to-machine co- mmunications (CM2M) is a new paradigm to improve the mobile services, which have drawn considerable attention in industry and academia. In this paper, we consider the quality of protection (QoP) of information transmission in cloud-assisted CM2M communications. In such an environment, the secondary M2M system intends to share the primary spectrum on the condition that the secondary transmitter (ST) has to relay the primary message. However, the ST is a low-energy device which adopts the energy harvesting technique to power itself. In particular, we focus on secure information transmission for the primary system when the secondary users (SUs) are the potential eavesdroppers. We aim to jointly design power splitting and secure beamforming to maximize the secondary M2M system data rate subject to the secrecy requirement of the primary system and the ST power constraint. To solve this non-convex problem, we propose a computationally efficient two-stage optimization approach. Simulation results demonstrate that our proposed scheme achieves a significant transmission rate of the secondary M2M system while provides a high secrecy rate for the primary system compared to the scheme without energy harvesting.     

A spatiotemporal super-resolution algorithm for a hybrid stereo video system

This paper presents a spatiotemporal super-resolution method to enhance both the spatial resolution and the frame rate in a hybrid stereo video system. In this system, a scene is captured by two cameras to form two videos, including a low spatial resolution with high-frame-rate video and a high spatial resolution with low-frame-rate video. For the low-spatial-resolution video, the low-resolution frames are spatially super-resolved by the high-resolution video via the stereo matching, the bilateral overlapped block motion estimation, and the adaptive overlapped block motion compensation algorithms, while for the low-frame-rate video, those missed frames are interpolated using the high-resolution frames obtained by fusing the disparity compensation and the motion compensation frame rate up-conversion. Experimental results demonstrate that the proposed mixed spatiotemporal super-resolution method has a more significant contribution to both the subjective and objective qualities than the pure spatial super-resolution or the frame rate up-conversion.     

红外跟踪测量系统动态精度偏置检测方法研究

利用动态精度靶标能够确定被检测光电经纬仪回转中心的空间位置.提出了动态精度偏置检测方法,实现对非中心红外跟踪系统动态角测量精度的检测,由于不需要在轴端镜头上加装分光镜组,不改变系统的转动惯量,提升了非中心系统的动态检测精度.对检测数据处理,验证了该方法的有效性,并在某型光电经纬仪检测中得到应用.     

特征点匹配法实现汽车牌照的快速识别

本文在模板匹配法的基础上，根据汽车牌照本身的特性，提出了特征点匹配识别的方法，选取能够区分各字符的若干特征点，进面最值得到各字符的编码、通过判断达到识别汽车牌照的目的。本方法减少了冗余信息，大大提高了识别速度。     

3mm波段中频比较辐射计的研究

叙述了3mm中频比较辐射计的工作原理、电路结构、特性分析及实验结果，研究表明，这种辐射计不需3mm开关，其稳定性优于全功率辐射计，而成本和尺寸均与全功率辐射计相差不大，完全可以满足小型化、实用化的需要。     

含B闪锌矿三元系半导体BxIn1-xP和BxGa1-xP带隙理论预测

利用第一性原理计算方法,用广义梯度近似 (GGA) 处理电子之间的交换关联能,计算了BxIn1-xP,BxGa1-xP的带隙结构.BxIn1-xP的带隙弯曲参数b _Eg (Γ)=4.32eV,b_Eg(X) =1.8eV,它从直接带隙转变为间接带隙时B的含量为0.47;在小量B的掺入下,可使BxIn1-xP的带隙变小,在x=0.15时带隙达到最小值1.33eV.BxGa1-xP的带隙弯曲参数b_Eg(Γ)=1.37eV,b _Eg(X)=2.46eV,在整个组分内是间接带隙.BxIn1-x-P,BxGa1-xP有较大的带隙弯曲参数,是由于组成它们的二元系之间存在较大的晶格失配.     

SiC外延工艺中的气体流体模型

构建了热壁CVD法生长α-SiC外延的气体流体力学模型,并通过COMSOL模拟软件计算对基座的几何形状进行设计.结果表明基座的几何形状改变,影响衬底表面的气流分布;基座有一定的倾斜角度,可以使其表面气流分布均匀,有利于得到高质量的α-SiC外延片.     

633 nm激光辐射沿心包经脉线传输特性的实验测量

基于生物医学光学的原理与测量技术,设计了无损检测光波沿人体经脉线传输特性的实验方案,测量了633 nm光波沿心包经脉线和旁开非经脉线方向的光波辐出度特征,分析了光波调制频率对测量光信号的影响.实验对象为20例健康青年志愿者,实验结果采用医学统计软件SPSS13.0进行统计分析.研究初步表明:光波沿经脉线方向和非经脉线方向的光强度衰减差异明显(P＜0.01),且光波沿经脉线方向的衰减小于非经脉线方向;在低频范围内,光波调制频率越大,光波沿经脉线的衰减越快.     

A 1-V 86-mW-RX 53-mW-TX Single-Chip CMOS Transceiver for WLAN IEEE 802.11a

A 1-V WLAN IEEE 802.11a CMOS transceiver integrates all building blocks on a single chip including a transformer-feedback VCO and a stacked divider for the frequency synthesizer and 8-bit IQ ADCs and 8-bit IQ DACs. Fabricated in a 0.18-mum CMOS process and operated at a single 1-V supply, the receiver and the transmitter consume 85.7 mW and 53.2 mW, including the frequency synthesizer, respectively. The total chip area with pads is 12.5 mm².