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In the present study, a total of eight sourdough samples were collected from three different bakeries at two different times in Turkey. Also, laboratory-scale sourdough production was conducted by daily back-slopping for 7 days. Microbiological and chemical properties of the sourdoughs were investigated. Yeast species in the sourdoughs were identified by subjecting all presumptive yeast cultures to internal transcribed spacer region amplification of the 5.8S rRNA gene, restriction fragment length polymorphism analysis using Hae III, Hha I, and Hinf I endonucleases, and sequence analysis of the D1/D2 domain of the 26S rDNA gene. A total of seven profiles were determined according to the restriction fragments. Totally, 148 yeast isolates were identified at the species level (≥400 bp , 99% identity) as Saccharomyces cerevisiae (106), Kazachstania bulderi (11), Pichia fermentans (nine), Pichia membranifaciens (eight), Kazachstania servazzii (seven), Kazachstania unispora (four), and Hanseniaspora valbyensis (three). Although collected sourdoughs were produced without using baker's yeast, S. cerevisiae was the most frequently isolated yeast species. This can be related to the contamination of the bakery environment with commercial baker's yeast during the production of other bakery products. The pH and acidity levels of the collected sourdough samples ranged from 3.71 to 3.96 and 6.78 to 23.93 mL 0.1 N NaOH/10 g dough, respectively. Mean values of the content of maltose + sucrose, glucose, fructose, lactic acid, and acetic acid were 2.43, 1.57, 2.67, 7.30, and 1.40 g/kg, respectively. Due to the artisan and region-dependent handling of the sourdough, different biochemical patterns were observed among the collected samples.  相似文献   
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The volatile oils of flowers (TFE), leaves (TLE) and stems (TSE) of Tripleurospermum conoclinium (Boiss. & Balansa) Hayek were obtained by hydrodistillation method for 3 hours with Clevenger-type apparatus. Chemical composition of oils was determined by GC-MS analyses. Antidiabetic, anti-inflammatory and antioxidant activities of oils were tested by α-glucosidase inhibitory, 5-lipoxygenase inhibitory and DPPH/ABTS radical scavenging methods, respectively. Eighty-five constituents were identified in flower oil (78.3%). The major compounds of flower oil were artemisia ketone (10.5%), β-oplopenone (6.0%), tricosane (4.5%), terpinen-4-ol (3.7%) and artemisia alcohol (3.5%). Sixty-two constituents were identified in leaf oil (67.0%). 1-octadecanol (11.5%), caryophyllene oxide (5.7%), elemol (3.9%), phytol (3.5%), ledene oxide (3.3%) and artemisia ketone (3.1%) were found as main constituents in the leaf oil. Forty-six constituents were determined in the stem oil (75.1%). The main compounds of the stem oil were 1-octadecanol (11.1%), β-oplopenone (5.5%), ledene oxide (5.5%), caryophyllene oxide (4.4%) and phytol (3.0%). TLE and TSE displayed strong antidiabetic activity with IC50 values of 21.30 and 29.57 µg/mL against α-glucosidase enzyme, respectively. TLE showed the best anti-inflammatory activity with an IC50 value of 91.02 µg/mL while TFE and TSE exhibited moderate activity with IC50 values of 186.50 and 257.10 µg/mL, respectively. TFE and TLE were found to possess moderate antioxidant activity with IC50 values of 572.80 and 385.80 µg/mL against ABTS radical while all essential oils showed weak activity against DPPH radical. To the best of our knowledge, this is first report on chemical composition and biological activities of T conoclinium essential oils.  相似文献   
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Citric acid (CA) productivity by Yarrowia lipolytica dependents on strain type, carbon source, carbon to nitrogen (C/N) molar ratio as well as physicochemical conditions (pH, temperature, oxygen transfer rate, etc.). In the current study, 10 different Ylipolytica strains were first challenged in shake-flask culture for CA production in a glucose-based media under nitrogen-limited conditions. For the most promising one, strain K57, CA productivity was monitored during culture in batch bioreactor for three initial C/N molar ratio (167, 367, and 567 Cmol/Nmol). The highest CA yield (0.77 g/g glucose), titre (72.3 g/L CA), and productivity (0.04 g/g.h) were found for C/N ratio of 367. However, the highest growth rate was obtained for an initial C/N ratio of 167. From these results, Ylipolytica strain K57 could be considered to produce CA at higher titre on glucose-based medium in batch bioreactor than others Ylipolytica strain reported in the literature.  相似文献   
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Software and Systems Modeling -  相似文献   
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The effects of La content on the mechanical properties and corrosion behavior of as-cast Mg-Zn-Zr (ZK) magnesium alloys were investigated. The results showed that La addition to ZK60 alloy resulted in a considerable grain refinement and an increase in the volume fraction of second phases by formation of Mg-Zn-La ternary phase. The tensile tests were performed at both room and elevated temperatures. At room temperature, the yield and tensile strengths and elongation-to-fracture significantly increased with 0.2 wt.% La addition, then deteriorated slightly with increasing La additions above 0.2 wt.%. At 200 °C, the yield and tensile strengths continually improved and elongation-to-fracture decreased with increasing La content due to the presence of thermally stable Mg-Zn-La phase. Immersion and electrochemical corrosion tests revealed that the formation of Mg-Zn-La phases led to a higher amount of cathodic sites and preferential corrosion propagation, and thus a decrease in the corrosion resistance of ZK60 alloys.  相似文献   
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One of the important design elements for a good production system is material handling. In cases where it is not well-designed, it can be the bottleneck in the system. Moreover, it can cause a lot of wastes such as waiting time, idle time, and excessive transportation and cost. In this study, material handling in lean-based production environments is taken into account. Depending on the lean structure of the production systems such as being pull-based, smooth, and repetitive, delivering the materials to the stations periodically becomes important. At this point, milk-run trains are highly used in real applications since they enable the handling of required amount of materials on a planned basis. With this study, it is aimed to develop a specific model for milk-run trains which travel periodically in the production environment on a predefined route in equal cycle times with the aim of minimizing work-in-process and transportation costs. Since the milk-run trains having equal cycle times start their tours at the same time intervals, it becomes simple to manage them. For this reason, they are used in lean production systems where level scheduling is performed. The developed model is based on mixed-integer linear programming, and since it is difficult to find the optimum solution due to the combinatorial structure of the problem, a novel heuristic approach is developed. A numerical example is provided so as to show the applicability of the mathematical model and the heuristic approach.  相似文献   
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