Scanning electron microscopy of penile papillae in intact and castrated rats

Oviduct segments from infundibulum, magnum, uterus, utero-vaginal junction and vagina of actively laying hens at preoviposition time were tested for their contractile reaction to prostaglandin E1 by in vitro or in vivo methods. Maximum stimulatory response was observed from the muscular strips of the proximal oviduct segment (infundibulum) and a complete relaxation was recorded from the distal part (vagina) at molar concentrations of 1.4 X 10(-7), 3.4 X 10(-7) and 7.0 X 10(-7). The uterine strips reacted with a stimulatory response at higher concentrations (1.4 X 10(-6) and 2.8 X 10(-6) moles), but lacked any significant change at lower concentrations. The uterovaginal muscular strips showed a mild but prolonged inhibitory response, while the magnum responded with a significant increase in the luminal pressure when tested in vivo. It is concluded that PGE1 exerts a stimulatory effect on the uterus to initiate transport of the egg to subsequent segments (utero-vaginal junction and vagina), which relax under PGE1 influence and allow passage of the egg by pressure differences.     

Trisomy 9p syndrome and XYY syndrome in siblings

An equimolar mixture of a xanthurenic acid-insulin complex and ZnSO4 was separated into an insulin peak and a peak containing xanthurenic acid (XA) and Zn by Sephadex G-75 column chromatograpy. XA and di-[L-histidino]-zinc (II) readily combined to produce di-[L-histidino]-di-xanthurenato zinc (II) (His2-Zn2+-XA2). By increase in the concentration of Zn2+ ions, XA was removed from di-[L-histidino]-di-xanthurenato zinc (II) (His2-Zn2+-XA2) as XA-Zn2+. The XA-insulin complex showed dcreased relative intensity of fluorescence compared with the Zn-insulin when excited at a wavelength of 284 nm. The difference spectrum between native Zn-insulin and XA-insulin complexes showed a slight red shift. A diffference in the CD spectrum between native Zn-insulin and XA-insulin complexes was observed.     

Transcription of the genome of adenovirus type 12. IV. Maps of stable late RNA from productively infected human cells

From human KB cells productively infected with adenovirus type 12, mRNA and stable nuclear RNA were isolated late (42 h) after infection. Using restriction endonuclease fragments of adenovirus type 12 DNA, mRNA and stable nuclear RNA sequences were mapped on the viral genome. Late after infection, preferentially the r (= rightward) strand is transcribed into stable nuclear RNA, whereas the l (= leftward) strand is expressed only to a minor extent. Adenovirus type 12-specific mRNA originates from the following sections on the viral genome: 0 to 0.11, 0.18 to 0.20, 0.27 to 0.49, 0.56 to 0.63, 0.68 to 0.84, and 0.89 to 0.92 fractional length units on the r strand and 0.11 to 0.16, 0.22 to 0.27, 0.50 to 0.54, 0.62 to 0.66, 0.855 to 0.865, and 0.93 to 1.0 fractional length units on the l strand. Self-complementary viral RNA isolated at 42 h postinfection anneals to 70 to 80% of each strand of the viral genome.     

In vitro binding of pentobarbital sodium--a component of nembutal--to human blood serum albumins

Comparisons were made of the sexual behaviour of sham-operated male hamsters and castrated males receiving testosterone, dihydrotestosterone or androstenedione (1-5 mg/week), or oil alone. Tests of short duration (10 min) were conducted at Week 3 (when the animals were sexually naive), Week 6 and Week 9. Sham-operated males showed marked increases in many elements of behaviour between Weeks 3 and 9, while castrated males receiving no androgen replacement showed marked decreases. Males receiving each of the three androgens showed marked increases in behaviour, but androstenedione-treated males showed less facilitation of sexual behaviour than controls. Dihydrotestosterone was as effective as testosterone. The three androgens were comparable in maintaining seminal vesicle weight after castration and in preventing the customary rise in pituitary and body weights. These data suggest that, unlike the situation in the rat, aromatization of androgens is unnecessary for the display of sexual behaviour in the hamster.