Selective solid-phase extraction of rare earth elements by the chemically modified Amberlite XAD-4 resin with azacrown ether

A selective solid-phase extraction procedure using chemically modified Amberlite XAD-4 with monoaza dibenzo 18-crown-6 ether was investigated for the preconcentration and separation of La(III), Nd(III) and Sm(III) in synthetic solution. Before loading samples on synthesized adsorbent adjust pH 4.5 by suitable buffer solution. The adsorbed rare earth elements were eluted by 2 M hydrochloric acid. Various parameters like preconcentration, breakthrough capacity, flow rate were investigated. The limits of detection (n = 5) and limits of quantification (n = 5) for La(III), Nd(III) and Sm(III) were founded 3.9, 4.2 and 7.4 μg L^?1 and 13, 15 and 26 μg L^?1, respectively. The eluted metal ions were determined by ICP-AES.     

Survival of Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes on Inoculated Almonds and Pistachios Stored at -19, 4, and 24° C

The survival of Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes was determined on almonds and pistachios held at typical storage temperatures. Almond kernels and inshell pistachios were inoculated with four- to six-strain cocktails of nalidixic acid-resistant Salmonella, E. coli O157:H7, or L. monocytogenes at 6 log CFU/g and then dried for 72 h. After drying, inoculated nuts were stored at -19, 4, or 24°C for up to 12 months. During the initial drying period after inoculation, levels of all pathogens declined by 1 to -log CFU/g on both almonds and pistachios. During storage, moisture content (4.8%) and water activity (0.4) of the almonds and pistachios were consistent at -19°C; increased slowly to 6% and 0.6, respectively, at 4°C; and fluctuated from 4 to 5% and 0.3 to 0.5 at 24°C, respectively. Every 1 or 2 months, levels of each pathogen were enumerated by plating; samples were enriched when levels fell below the limit of detection. No reduction in population level was observed at -19 or 4°C for either pathogen, with the exception of E. coli O157:H7-inoculated almonds stored at 4°C (decline of 0.09 log CFU/g/month). At 24°C, initial rates of decline were 0.20, 0.60, and 0.71 log CFU/g/month on almonds and 0.15, 0.35, and 0.86 log CFU/g/month on pistachios for Salmonella, E. coli O157:H7, and L. monocytogenes, respectively, but distinct tailing of the survival curves was noted for both E. coli O157:H7 and L. monocytogenes.     

Microstructure and protein digestibility of beef: The effect of cooking conditions as used in stews and curries

Beef meat was cooked at 373 K for 10 and 30 min to investigate the effect of the cooking conditions generally used during beef stew and curry preparation on protein digestibility. The cooked meats, along with a raw control, were digested using an in vitro digestion model to simulate gastric and small-intestinal conditions. Samples taken at different digestion times were analyzed using SDS-PAGE, RP-HPLC, ninhydrin assays for amino N and transmission electron microscopy. Simulated gastric conditions quickly led to the loss of basic sarcomere structure in raw meat myofibrils whereas the sarcomere structure of the compact cooked meat myofibrils remained intact after 30 min of gastric digestion. Prolonged cooking of meat (30 min) resulted in incomplete digestion of small MW (<10 kDa) peptides, as observed from SDS-PAGE. This agreed with the amount of ninhydrin-reactive amino N released during digestion, which decreased with an increase in cooking time. The RP-HPLC peak areas of the major identified amino acids (tryptophan, tyrosine and phenylalanine) also decreased with an increase in cooking time. This suggested the formation of “limit peptides” during prolonged cooking of beef, which were not further broken down into free amino acids by digestive enzymes and therefore might not be bioavailable.     

Preparation of cobalt ferrite from the thermolysis of cobalt tris(malonato)ferrate(III)trihydrate precursor

Thermal decomposition of cobalt tris(malonato)ferrate(III)trihydrate precursor, Co₃[Fe(CH₂C₂O₄)₃]·3H₂O has been investigated from ambient temperature to 600 °C in static air atmosphere using various physico-chemical techniques, i.e. TG–DTG–DSC, XRD, Mössbauer and IR spectroscopic techniques. The precursor undergoes dehydration and decomposition simultaneously to yield cobalt malonate and iron(II) malonate intermediates at 205 °C. At higher temperature (325 °C) these intermediate species undergo exothermic decomposition to yield CoO and α-Fe₂O₃, respectively. Finally cobalt ferrite, CoFe₂O₄, has been obtained as a result of solid–solid reaction between Fe₂O₃ and CoO at a temperature (380 °C) much lower than that of ceramic method. SEM analysis of the final thermolysis product reveals the formation of monodisperse cobalt ferrite nano-particles with an average particle size of 45 nm. Magnetic studies show that these particles have a saturation magnetization of 3095 G and Curie temperature of 504 °C. Lower magnitude of these parameters as compared to the bulk values is attributed to the smaller particle size.     

A study of austenitization of SG iron

Austenitization process of three SG irons with varying compositions and as cast matrix microstructure has been studied at three austenitization temperatures of 850, 900 and 950C for different time periods. Microstructure, hardness and X-ray diffraction have been used to reveal the nature of dependence of the process on austenitization temperature, time and as cast structure. The optimum austenitization time is maximum for ferritic and minimum for pearlitic matrix.     

Optical and electrical characterization of conducting polymer-single walled carbon nanotube composite films

The optical and electrical properties of the conducting polymer poly(3-hexylthiophene) and single walled carbon nanotube (SWCNT) composites have been investigated. The composites were prepared by dispersing carbon nanotubes in the polymer matrix already dissolved in 1,2-dichlorobenzene. The optical absorption, photoluminescence (PL) and electrical conductivity of the composite was studied as a function of SWCNT concentration in the solution. The absorption coefficient of the polymer was found to be unaffected upto a SWCNT concentration 5% w/w. However a minor decrease in the absorption in visible region was observed for higher SWCNT concentrations. The intensity of PL emission from the composite was measured and was found to decrease with the increase in SWCNT concentration. For a SWCNT concentration of 30% w/w, ∼90% of the PL was quenched, indicating an ultra fast transfer of photoinduced charges from donor polymer to acceptor SWCNT. Direct current conductivity of the composite film was found to increase rapidly with the increase in SWCNT concentration and an increase of ∼5 orders of magnitude was observed for a 30% w/w concentration. The enhancement in conductivity is explained in terms of percolation theory with an estimated percolation threshold of 2% w/w.     

An efficacious “naked-eye” selective sensing of cyanide from aqueous solutions using a triarylmethane leuconitrile

A triarylmethane dye 1 detects CN^? from water and shows remarkable selectivity over other anions and dramatic color changes, in solution as well as when dyed. The pH stability of 1 in the range of 1.2–10.7 offers additional advantage in the sensing process under physiological conditions.     

Development of pitch-based carbon–copper composites

Carbon–copper composites with varying copper to carbon ratio of 0.66–1.5 (by weight) were developed from coal-tar-pitch-derived green coke (as such or modified with natural graphite) as carbon source and electrolytic grade copper powder at different heat treatment temperatures (HTTs) of 1000–1400 °C. The physical, mechanical, and electrical properties differ depending upon the HTT and also on copper to carbon ratio (Cu/C). The composites prepared at HTT of 1100 °C having Cu/C ratio of 0.66 and 0.9 exhibited a high bending strength of 150 and 140 MPa, bulk density of 2.63 and 2.81 gm/cm³, electrical resistivity of 1.6 and 0.96 m Ω cm and shore hardness of 88 and 84, respectively, in spite of well-known inadequate wettability between copper and carbon. Increasing the temperature from 1100 °C for processing of the composites deteriorated the properties mainly due to the loss of copper through melting above 1100 °C as revealed by X-ray, scanning electron microscopy, thermal analysis and EDAX studies.     

Characterization of Thermal-Sprayed HAP and HAP/TiO<Subscript>2</Subscript> Coatings for Biomedical Applications

In the present study, hydroxyapatite (HAP) coating along with HAP/TiO₂ coating has been deposited by high-velocity flame spray (HVFS) technique onto 316LSS. Titania was used as a bond coat and HAP as top coat in HAP/TiO₂ coating. The main aim of the study is to investigate the corrosion behavior of thermal spray coating of HAP and HAP/TiO₂ on steel. Electrochemical corrosion testing was carried out using potentiodynamic polarization test. The corrosion behavior of bare and as-sprayed specimens was analyzed in simulated body fluid known as Hank’s solution. As-sprayed specimens along with corroded specimens were further characterized by XRD, SEM/EDS, and x-ray mapping analysis. It was observed that the HAP/TiO₂ coating possessed higher microhardness (280 Hv) as compared to HAP coating (254 Hv). Surface roughness also got enhanced in case of HAP/TiO₂ coating (9.35 μm) as compared to pure HAP coating (7.37 μm). The porosity of the HAP coating was found to be higher than the bond coating. It was observed that the Ca/P ratio almost resembled that of the natural bone composition. The corrosion resistance of steel increased after the deposition of HAP and HAP/TiO₂ coatings. The maximum corrosion resistance was exhibited by HAP/TiO₂ coating.     

Response of laying Japanese quails to graded levels of essential amino acids profile with reduced dietary protein

The effect on egg production of graded levels of ideal amino acids, combined with reduced protein in the diet, was investigated in 312 laying quails aged 6–18 weeks. The quails were offered six diets, each of which contained one of three levels of amino acids (85, 100 and 115% of essential amino acids (EAAs)) together with 5% or without fishmeal (FM) (3 × 2 factorial design). Each diet was offered to 26 replicated groups of two quails each. Hen‐day and hen‐housed egg production did not differ as a result of EAA level, protein type or their interaction during the overall period of egg production. Egg weight improved linearly (P < 0.01) with increased EAA levels, while egg mass output per bird per day remained similar at the 100 and 115% EAA levels. Quails fed higher (100 and 115%) EAA levels had an improved feed conversion ratio (P < 0.01) compared to birds fed 85% EAA. The gain in body weight during the laying period was higher (P < 0.01) at the 100 or 115% than at 85% EAA levels The ratio of egg mass or egg mass and live weight gain, together, to protein intake improved (P < 0.01) linearly with a decrease in EAA levels in the diets, while better (P < 0.01) energy efficiency (EE, energy intake: egg mass) and net EE (energy intake: egg mass plus gain) was obtained in higher EAA levels (100 or 115%). Protein and energy efficiencies remained similar due to protein type or interaction. Shape index, albumen index, yolk index, yolk colour and relative shell weight did not differ due to EAA levels, protein type or their interaction. Eggs laid from quails fed diets with 100% EAAs without FM and 115% EAAs with or without FM had higher shell thickness than those on 85% EAAs irrespective of protein type. The retention of nitrogen and calcium retention was higher (P < 0.01) at the 115% EAA level. The results indicated a dietary level of 100% EAA (185 g kg^?1 crude protein (CP)) with 12.13 MJ kg^?1 was suitable for laying quails of 6–18 weeks of age. Copyright © 2007 Society of Chemical Industry