Cell-laden microengineered pullulan methacrylate hydrogels promote cell proliferation and 3D cluster formation

The ability to encapsulate cells in three-dimensional (3D) environments is potentially of benefit for tissue engineering and regenerative medicine. In this paper, we introduce pullulan methacrylate (PulMA) as a promising hydrogel platform for creating cell-laden microscale tissues. The hydration and mechanical properties of PulMA were demonstrated to be tunable through modulation of the degree of methacrylation and gel concentration. Cells encapsulated in PulMA exhibited excellent viability. Interestingly, while cells did not elongate in PulMA hydrogels, cells proliferated and organized into clusters, the size of which could be controlled by the hydrogel composition. By mixing with gelatin methacrylate (GelMA), the biological properties of PulMA could be enhanced as demonstrated by cells readily attaching to, proliferating, and elongating within the PulMA/GelMA composite hydrogels. These data suggest that PulMA hydrogels could be useful for creating complex, cell-responsive microtissues, especially for applications that require controlled cell clustering and proliferation.     

An algorithm for a cutting problem in window frame production

This research discusses the cutting problem encountered by a real-life window frame manufacturer. In the problem, four types of bars (upper, bottom, left and right) should be cut from raw material aluminium profiles for each window frame order. These bars must be cut such that trim loss is minimised. Moreover, the bars should be assigned to the same raw material profile if possible to increase productivity; otherwise, they should be assigned to neighbouring raw material profiles. Furthermore, the numbers of bar types as derived from a raw material profile should not be unbalanced because this scenario induces subsequent machine load imbalance. In this study, we develop a mixed integer programming model and a knapsack-based heuristic approach that minimises the weighted sum of trim loss, bar type imbalance and the degree of order spreading. The results of computational experiments demonstrate the effectiveness of the proposed algorithm, and the proposed approach outperforms the legacy system of the company. Thus, this method is currently being used by the firm in question.     

Macrochanneled poly (ɛ-caprolactone)/ hydroxyapatite scaffold by combination of bi-axial machining and lamination

A combination of bi-axial machining and lamination was used to fabricate macrochanneled poly (ɛ-caprolactone) (PCL)/hydroxyapatite (HA) scaffolds. Thermoplastic PCL/HA sheets with a thickness of 1 mm, consisting of a 40 wt% PCL polymer and 60 wt% HA particles, were bi-axially machined. The thermoplastic PCL/HA exhibited an excellent surface finish with negligible tearing of the PCL polymer and pull-out of the HA particles. The bi-axially machined sheets were laminated with a solvent to give permanent bonding between the lamina. This novel process produced three-directionally connected macrochannels in the dense PCL/HA body. The macrochanneled PCL/HA scaffold exhibited excellent ductility and reasonably high strength. In addition, good cellular responses were observed due to the osteoconductive HA particles.     

A normally off GaN n-MOSFET with Schottky-barrier source and drain on a Si-auto-doped p-GaN/Si

We have fabricated an enhancement-mode n-channel Schottky-barrier-MOSFET (SB-MOSFET) for the first time on a high mobility p-type GaN film grown on silicon substrate. The metal contacts were formed by depositing Al for source/drain contact and Au for gate contact, respectively. Fabricated SB-MOSFET exhibited a threshold voltage of 1.65 V, and a maximum transconductance(g/sub m/) of 1.6 mS/mm at V/sub DS/=5V, which belongs to one of the highest value in GaN MOSFET. The maximum drain current was higher than 3 mA/mm and the off-state drain current was as low as 3 nA/mm.     

Integral loop test and assessment of modified RELAP5/MOD3.3 for RCS coolant inventory during a LBLOCA

This study investigates experimentally and analytically the thermal hydraulic phenomena during the transition from design basis accident (DBA) to beyond-DBA, particularly, the depletion of core coolant inventory. To investigate the overall thermal hydraulic behavior after safety injection (SI) failure during a large-break loss-of-coolant accident (LBLOCA) in a cold leg, an integral loop test was performed at the Seoul National University Integral Test Facility (SNUF), which was scaled down to 1/6.4 in length and 1/178 in area from the advanced power reactor 1400 MWe (APR1400) according to the three-level scaling method. The plant condition at 200.0 s as the base case and those at 625.0 and 1950.0 s as test cases after the initiation of LBLOCA were applied as initial conditions in the experiments, respectively. The experimental results showed that the sweepout increased the coolant flow discharged to the break depending on the steam flow rate in intact cold legs and the initial downcomer coolant level and expedited the depletion of the core coolant inventory.In the meantime, since RELAP5/MOD3.3 uses the average properties of donor volume as those of its connected junction, this scheme causes the mass and the energy flux in a junction to be calculated incorrectly if significant phase separation occurs in the donor volume such as in the downcomer during the LBLOCA. The sweepout model was developed and implemented in RELAP5/MOD3.3 to improve its calculation of coolant inventory during the LBLOCA. To assess the applicability of the modified RELAP5/MOD3.3 to the actual system, the experiments in SNUF were simulated by both the original and the modified RELAP5/MOD3.3. The original one predicted the discharge flow rate at the break larger than that of the experiment. On the other hand, the modified one calculated the discharge flow rate more similar to that of the experiment than the original one did. As a result, the modified RELAP5/MOD3.3 reduced the coolant flow discharged to the break to delay the initiation time of heater heat-up after SI failure during LBLOCA in a cold leg. This improved RELAP5/MOD3.3 will support a more realistic thermal hydraulic analysis in an integrated analysis system.     

An enhanced role model for alleviating the role‐binding anomaly

Roles and role models have received much attention as useful concepts for developing highly reusable and dynamically evolvable systems. Role models belong to the category of collaboration‐based development techniques, but most of the existing approaches to role models do not explicitly incorporate the core principle of collaboration‐based developments as an essential property of their primary design goals. Consequently, the existing approaches still suffer from a problematic phenomenon that the structural and behavioral constraints defined in a role system can be violated during the role‐binding stage. We call such a problematic phenomenon the role‐binding anomaly. In order to alleviate the role‐binding anomaly, we propose an enhanced role model, in which all role instances and core objects can exist by themselves, namely, they can be developed, executed, and tested independently. Roles and core classes can be bound to each other at the instance level. In addition, the role system describes and encapsulates the behavior for dynamic reconfigurations among role instances. The enhanced role model is designed so as to be meaningful with respect to software engineering principles, rather than dynamic evolution. It also facilitates role model implementation using general programming languages (i.e. not supporting dynamic specialization) such as Java. To illustrate how the proposed role model makes such benefits, we develop a set of Java classes necessary for implementing the enhanced role model in the form of a Java package role, and present a simplified automatic teller machine system as an example application. Copyright © 2002 John Wiley & Sons, Ltd.     

Characterization of Condensed Tannins Purified From Legume Forages: Chromophore Production,Protein Precipitation,and Inhibitory Effects on Cellulose Digestion

To identify simple screening tools for selecting condensed tannin (CT)-containing forages as candidate sources for further study, CT were isolated from nine legumes, and their molecular weights (MW), chromophore production, capacity to precipitate bovine serum albumin (BSA) and Fraction 1 protein (Rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. Sources were as follows: leaves of sericea lespedeza (Lespedeza cuneata Dum.-Cours.), crown vetch (Coronilla varia L.), and sainfoin (Onobrychis viciifolia Scop.); stems of hedysarum (Hedysarum alpinum L.); seeds of alfalfa (Medicago sativa L.); and whole plants of birdsfoot trefoil (Lotus corniculatus var. corniculatus L.) and three varieties of big trefoil (Lotus pedunculatus Cav.), viz., Lotus uliginosus Schkuhr, L. uliginosus var. glabriusculus, and L. uliginosus var. villosus. Molecular weights and sizes (degrees of polymerization) of the CT varied considerably within and among plant species. Average MW ranged from 3036 Da (crown vetch) to 7143 Da (lespedeza). All CT exhibited greater capacity (w/w basis) to bind alfalfa Rubisco than BSA. Relative astringencies (μg CT required to precipitate 1 mg protein) against BSA ranged from 262.5 for CT from lespedeza to 435.5 for CT from L. corniculatus, and against Rubisco, from 49.6 (sainfoin) to 108.2 (alfalfa seed). Including CT at 300 μg/ml in cultures of Fibrobacter succinogenes reduced digestion of cellulose filter paper by 19.8% (sainfoin) to 92.4% (crown vetch) and increased the specific activity of cell-associated endoglucanase. There were no correlations between inhibitory effects of CT on filter paper digestion and (1) chromophore formation during CT assay by butanol–HCl, vanillin–HCl, or H₂SO₄; (2) precipitation of BSA or alfalfa Rubisco; and (3) MW of CT. The most inhibitory CT for cellulose digestion included those with broad and with narrow MW distributions. Sainfoin was the most desirable source of CT, as it had the highest capacity to bind alfalfa protein and was least inhibitory to cellulose digestion by F. succinogenes. This study suggests that these properties are not easily defined via chemical means, and that biological assays using rumen bacteria may help identify those CT with properties of nutritional interest.     

Treatment of landfill leachate by a pilot-scale modified Ludzack-Ettinger and sulfur-utilizing denitrification process.

Nitrogen removal efficiency of a pilot-scale system consisted of Modified Ludzack-Ettinger (MLE) followed by sulfur-utilizing denitrification (SUDNR) process was evaluated with a landfill leachate. For SUDNR, a down-flow mode sulfur packed bed reactor (SPBR) filled with sulfur and limestone particles was used. Although total nitrogen removal efficiency of the MLE process was about 80% at the recycle ratio of 4, effluent contained 350-450 mg/L NO(3-)-N. Up to a loading rate of 1.2 kg NO(3-)-N/m3-day, the SPBR could achieve complete removal of nitrate, and nitrate removal rate was kept to that level even at higher loading rate. When a COD/N ratio of MLE process was maintained at 2 instead of 4, more organics with molecular weight less than 500 were utilized for heterotrophic denitrification although denitrification was not complete with the lack of electron donors. Clogging in the SPBR, mainly by the accumulation of nitrogen gas in the pores, could easily be removed by introducing the effluent in an upward direction for 1 min at 1 hr intervals. The proposed treatment system could achieve nitrate free effluent with a slight increase in chemical cost. Furthermore, depending on further COD removal requirement after biological treatment, the proposed treatment system can be an economical solution.