Good operations practice supervisor profiles in cell-centric manufacturing

The selection and implementation of good operations practice cannot be undertaken in isolation; it must consider the enterprise context. The aim of this paper is to describe a robust process for the development of specific environment good operations practice role profiles for supervisors, and illustrate this through a case study within a complex cell-centric manufacturing environment. The approach identifies the activities undertaken by a cell leader and team leader in a given manufacturing organization, and also by a good operations practice cell leader and team leader in a relevant external organization. Then via a survey of operations managers and functional managers, those activities that a cell leader and team leader should do within the given manufacturing organization are identified. The approach enables the contextual adoption of good operations practice, develops supervisor role profiles which allows them to be proficient within their task domains, contributes to attaining agreement amongst operational and functional management, and has applications across cell-centric manufacturing. The potentially significant impacts available across cell-centric manufacturing are illustrated by the finding that following this examination of operations practice in the case study company two-thirds were classified as requiring change.     

Influence of omega fatty acids on skin permeation of a coenzyme Q10 nanoemulsion cream formulation: characterization,in silico and ex vivo determination

Objective: The aim of this study was to develop a coenzyme Q10 nanoemulsion cream, characterize and to determine the influence of omega fatty acids on the delivery of coenzyme Q10 across model skin membrane via ex vivo and in silico techniques.

Methods: Coenzyme Q10 nanoemulsion creams were prepared using natural edible oils such as linseed, evening primrose, and olive oil. Their mechanical features and ability to deliver CoQ10 across rat skin were characterized. Computational docking analysis was performed for in silico evaluation of CoQ10 and omega fatty acid interactions.

Results: Linseed, evening primrose, and olive oils each produced nano-sized emulsion creams (343.93–409.86?nm) and exhibited excellent rheological features. The computerized docking studies showed favorable interactions between CoQ10 and omega fatty acids that could improve skin permeation. The three edible-oil nanoemulsion creams displayed higher ex vivo skin permeation and drug flux compared to the liquid-paraffin control cream. The linseed oil formulation displayed the highest skin permeation (3.97?±?0.91?mg/cm²) and drug flux (0.19?±?0.05?mg/cm²/h).

Conclusion: CoQ10 loaded-linseed oil nanoemulsion cream displayed the highest skin permeation. The highest permeation showed by linseed oil nanoemulsion cream may be due to the presence of omega-3, -6, and -9 fatty acids which might serve as permeation enhancers. This indicated that the edible oil nanoemulsion creams have potential as drug vehicles that enhance CoQ10 delivery across skin.     

Autonomous terrain-following for unmanned air vehicles

This paper presents an integrated guidance and control design scheme for an unmanned air vehicle (UAV), and its flight test results. The paper focuses on the longitudinal control and guidance aspects, with particular emphasis on the terrain-following problem. An introduction to the mission, and the terrain-following problem is given first. Waypoints for climb and descent are defined. Computation of the reference trajectory in the vertical plane is discussed, including a terrain-following (TF) algorithm for real-time calculation of climb/descent points and altitudes. The algorithm is particularly suited for online computation and is therefore useful for autonomous flight. The algorithm computes the height at which the vehicle should fly so that a specified clearance from the underlying terrain is always maintained, while ensuring that the vehicle’s rate of climb and rate of descent constraints are not violated. The output of the terrain-following algorithm is used to construct a smooth reference trajectory for the vehicle to track. The design of a robust controller for altitude tracking and stability augmentation of the vehicle is then presented. The controller uses elevators for pitch control in the inner loop, while the reference pitch commands are generated by the outer altitude control loop. The controller tracks the reference trajectory computed by the terrain-following algorithm. The design of an electromechanical actuator for actuating the control surfaces of the vehicle during flight is also discussed. The entire guidance and control scheme is implemented on an actual experimental vehicle and flight test results are presented and discussed.     

Antioxidant activity of ginger extract in sunflower oil

The antioxidant activity of dichloromethane extract from ginger was evaluated during 6 months of storage of refined sunflower oil at 25 and 45 °C. Free fatty acid (FFA) content, peroxide value (POV) and iodine value (IV) were used as criteria to assess ginger extract as an antioxidant. After 6 months of storage at 45 °C, sunflower oil containing 1600 and 2400 ppm ginger extract showed lower FFA contents (0.083 and 0.080%) and POVs (24.5 and 24.0 meq kg^?1) than the control sample (FFA contents 0.380%, POV 198.0 meq kg^?1). Sunflower oil containing 200 ppm butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) showed FFA contents of 0.089 and 0.072% and POVs of 26.5 and 24.7 meq kg^?1 respectively after 6 months of storage at 45 °C. Similarly, after 6 months of storage at 45 °C, IVs of sunflower oil containing 1600 and 2400 ppm ginger extract were 80 and 92 respectively, higher than that of the control sample (53). However, IVs of sunflower oil treated with 200 ppm BHA and BHT were 94 and 96 respectively after 6 months of storage at 45 °C. These results illustrate that ginger extract at various concentrations exhibited very strong antioxidant activity, almost equal to that of synthetic antioxidants (BHA and BHT). Ginger extract also showed good thermal stability and exhibited 85.2% inhibition of peroxidation of linoleic acid when heated at 185 °C for 120 min. Therefore the use of ginger extract in foods is recommended as a natural antioxidant to suppress lipid oxidation. © 2003 Society of Chemical Industry     

Testing software components for integration: a survey of issues and techniques

Component‐based development has emerged as a system engineering approach that promises rapid software development with fewer resources. Yet, improved reuse and reduced cost benefits from software components can only be achieved in practice if the components provide reliable services, thereby rendering component analysis and testing a key activity. This paper discusses various issues that can arise in component testing by the component user at the stage of its integration within the target system. The crucial problem is the lack of information for analysis and testing of externally developed components. Several testing techniques for component integration have recently been proposed. These techniques are surveyed here and classified according to a proposed set of relevant attributes. The paper thus provides a comprehensive overview which can be useful as introductory reading for newcomers in this research field, as well as to stimulate further investigation. Copyright © 2006 John Wiley & Sons, Ltd.     

Microscopic melanoma detection and classification: A framework of pixel‐based fusion and multilevel features reduction

The numbers of diagnosed patients by melanoma are drastic and contribute more deaths annually among young peoples. An approximately 192,310 new cases of skin cancer are diagnosed in 2019, which shows the importance of automated systems for the diagnosis process. Accordingly, this article presents an automated method for skin lesions detection and recognition using pixel‐based seed segmented images fusion and multilevel features reduction. The proposed method involves four key steps: (a) mean‐based function is implemented and fed input to top‐hat and bottom‐hat filters which later fused for contrast stretching, (b) seed region growing and graph‐cut method‐based lesion segmentation and fused both segmented lesions through pixel‐based fusion, (c) multilevel features such as histogram oriented gradient (HOG), speeded up robust features (SURF), and color are extracted and simple concatenation is performed, and (d) finally variance precise entropy‐based features reduction and classification through SVM via cubic kernel function. Two different experiments are performed for the evaluation of this method. The segmentation performance is evaluated on PH2, ISBI2016, and ISIC2017 with an accuracy of 95.86, 94.79, and 94.92%, respectively. The classification performance is evaluated on PH2 and ISBI2016 dataset with an accuracy of 98.20 and 95.42%, respectively. The results of the proposed automated systems are outstanding as compared to the current techniques reported in state of art, which demonstrate the validity of the proposed method.     

Association Mechanism and Conformational Changes in Trypsin on Its Interaction with Atrazine: A Multi- Spectroscopic and Biochemical Study with Computational Approach

Atrazine (ATR) is a herbicide globally used to eliminate undesired weeds. Herbicide usage leads to various adverse effects on human health and the environment. The primary source of herbicides in humans is the food laced with the herbicides. The ATR binding to trypsin (TYP) was investigated in this study to explore its binding potential and toxicity. In vitro interaction of ATR with TYP was studied using multi-spectroscopic methods, molecular docking, and enzyme kinetics to explore the mechanism of binding for the TYP-ATR system. The TYP-ATR complex revealed binding constants (10³ M⁻¹), suggesting a moderate binding. The free energy for the TYP-ATR complexes was negative, suggesting a spontaneous interaction. Thermodynamic parameters enthalpy (ΔH) and entropy (ΔS) obtained positive values for the TYP-ATR system suggesting hydrophobic interactions in the binding process. Micro-environmental and conformational changes in TYP molecules were induced on interaction with ATR. Reduced catalytic activity of TYP was observed after interaction with ATR owing to the changes in the secondary structure of the TYP.