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991.
992.
The cost of electricity and operating reliability in heavy industry can be key in determining which processes and plants survive in today's competitive market. This paper provides an insight into the planning and implementation of several significant cost saving and reliability improvements at a turn-of-the-century steel mill in Ohio. A clear understanding of what to look for and how to proceed with such projects should provide motivation for others considering similar improvements 相似文献
993.
It is shown that the unique coherence properties of mode-locked lasers can be exploited to provide new opportunities for high-speed data transmission. Using passive Mach-Zehnder optical delay stages, very high bit rate optical data streams constructed from asynchronously combined transmitters can be demultiplexed directly in the optical domain, without imposing restrictions on the source wavelength. Potential implementation and performance of the technique are discussed.<> 相似文献
994.
C Gargalidis-Moudanos A Remaury N Pizzinat A Parini 《Canadian Metallurgical Quarterly》1997,51(4):637-643
Previous studies have shown that a subpopulation of the catecholamine-degrading enzymes monoamine oxidase (MAO) A and B holds a previously unknown regulatory site, the I2-imidazoline binding site (I2BS). In the present work, we characterized the isoforms of monoamine oxidases expressed in the rabbit renal proximal tubule, defined their relationship with I2BS, and investigated the ability of I2BS ligands to inhibit enzyme activity in intact cells. Two findings indicate that MAO-B is the predominant isoform expressed in the renal proximal tubule cells: 1) Western blot performed with an anti-MAO-A/MAO-B polyclonal antiserum revealed a single 55-kDa band corresponding to MAO-B; 2) enzyme assays showed an elevated MAO-B activity ([14C]beta-phenylethylamine oxidation: Vmax = 1.31 +/- 0.41 nmol/min/mg protein), whereas MAO-A activity was only detectable ([14C]5-HT oxidation: Vmax = 80.3 +/- 19 pmol/min/mg protein). Photoaffinity labeling with the I2BS ligand [125I]2-(3-azido-4-iodophenoxy)-methylimidazoline revealed a single 55-kDa band, which indicates that MAO-B of the renal proximal tubule cells holds the I2 imidazoline binding site. [3H]Idazoxan binding studies and enzyme assays showed that, in intact cells, I2BS ligands bind to and inhibit MAO-B. Indeed, the increase in the accessibility of intracellular compartment by cell permeabilization did not enhance [3H]idazoxan binding, which indicates that, in intact cells, intracellular I2BS are fully occupied by imidazoline ligands. In addition, enzyme assays showed that incubation of proximal tubule cells with imidazoline ligands leads to a complete, dose-dependent inhibition of MAO activity. These data show the predominant expression of MAO-B in rabbit renal proximal tubule and its regulation by imidazoline ligands in intact cells. 相似文献
995.
A multilevel soliton communication system is proposed and assessed. In this system, at the transmitter end each channel transmits its data via fundamental solitons with a pre-specified amplitude (i.e., soliton width). At the receiver end we take advantage of the sensitive relationship between the amount or fundamental soliton self-wavelength shift and the width of the soliton in the subpicosecond region. We first compress the incoming soliton noises to the subpicosecond level and pass them through a short length of fiber at the end of which the pulses have become separated in the wavelength domain since each soliton, corresponding to a data channel, has experienced a different Raman self-wavelength shift. The channels are then easily separated by optical filters. We have derived the design constraint relations for such a system. We have then heuristically designed a 40 Gbs (four channels) system for a 1000 km propagation distance (total data-rate distance product of 40 Tb/km). Numerical simulations and noise analyses have verified the feasibility and practicality of the proposed system with very good design margins. The wavelength jitter is found to be much smaller than the desired filter spacing, and thus its contribution to the bit error rate is negligible. We also argue that the system is more tolerant to Gordon-Haus timing jitter than conventional TDM soliton systems. The system is all fiber and is, therefore very cost effective as it does not require sophisticated electro-optic and microwave circuits for demultiplexing. The system can potentially operate at much higher speeds than those achievable in conventional soliton systems and it can be used in parallel with WDM soliton system 相似文献
996.
Ernst L.J. Hinman W.L. Quam D.H. Thorp J.S. 《Power Delivery, IEEE Transactions on》1992,7(4):1834-1852
The authors describe the relaying concepts of charge comparison. Charge comparison is a new transmission line protection system. It is a form of current differential relaying. Charge comparison largely resolves the traditional problems of current differential relaying of transmission lines, which are that protection is lost if a channel fails, a large channel capacity is required, and precise channel delay compensation is required. This technique is suitable for the protection of two- or three-terminal AC transmission lines, of all lengths and voltage levels, with or without series of shunt compensation, with three-hole or single-pole tripping 相似文献
997.
998.
999.
M Tuena de Gómez-Puyou F Sandoval JJ García A Gómez-Puyou 《Canadian Metallurgical Quarterly》1998,255(1):303-308
Soluble mitochondrial F1 and F1 in complex with the natural ATPase inhibitor protein (F1-IP) catalyze the spontaneous synthesis of [gamma-32P]ATP from medium [32P]phosphate and enzyme-bound ADP when incubated in media with dimethylsulfoxide (Me2SO); under these conditions, the synthesized [gamma-32P]ATP is not released into the media, it remains tightly bound to the enzymes [Gómez-Puyou, A., Tuena de Gómez-Puyou, M. & de Meis, L. (1986) Eur. J. Biochem. 159, 133-140]. Some of the characteristics of the synthesized [gamma-32P]ATP were studied in F1 and F1-IP (ATPase activities of 70 and 1-3 micromol x min(-1) x mg(-1), respectively). In Me2SO media, gamma-phosphate of synthesized ATP in F1 or F1-IP exchanges with medium phosphate. From the rates of the exchange reaction, the half-times for hydrolysis of the synthesized ATP in F1 and F1-IP were calculated: 45 min and 58 min for F1 and F1-IP, respectively. The course that synthesized [gamma-32P]ATP follows after dilution of the Me2SO synthetic mixture with aqueous buffer was determined. After dilution, the half-life of synthesized ATP in F1 was less than 1 min. In F1-IP, ATP was also hydrolyzed, but at significantly lower rates. In F1-IP, dilution also produced release of the synthesized [gamma-32P]ATP. This was assayed by the accessibility of [gamma-32P]ATP to hexokinase. About 25% of [gamma-32P]ATP synthesized in F1-IP, but not in F1, was released into the media after dilution with aqueous buffer that contained 20 mM phosphate. Release of tightly bound ATP required the binding energy of phosphate and solvation of F1-IP, however, the particular kinetics of F1-IP were also central for medium ATP synthesis in the absence of electrochemical H+ gradients. 相似文献
1000.
F Gaymard G Pilot B Lacombe D Bouchez D Bruneau J Boucherez N Michaux-Ferrière JB Thibaud H Sentenac 《Canadian Metallurgical Quarterly》1998,94(5):647-655
SKOR, a K+ channel identified in Arabidopsis, displays the typical hydrophobic core of the Shaker channel superfamily, a cyclic nucleotide-binding domain, and an ankyrin domain. Expression in Xenopus oocytes identified SKOR as the first member of the Shaker family in plants to be endowed with outwardly rectifying properties. SKOR expression is localized in root stelar tissues. A knockout mutant shows both lower shoot K+ content and lower xylem sap K+ concentration, indicating that SKOR is involved in K+ release into the xylem sap toward the shoots. SKOR expression is strongly inhibited by the stress phytohormone abscisic acid, supporting the hypothesis that control of K+ translocation toward the shoots is part of the plant response to water stress. 相似文献