猴头菌转化银杏叶提取物(EGB)工艺条件的研究

在前期优化培养基的基础上,本实验比较了摇瓶的温度、pH、装液量和发酵罐培养的通气量、转速和培养时间等不同培养条件对猴头菌转化EGB生物活性的影响。结果显示,含有0.5%的EGB培养基中,猴头菌转化EGB温度为25～30℃,初始pH范围为5.5～6.5,此时对非酶糖基化反应的抑制率较高。同时添加10g/LCaCO3可稳定pH,提高发酵液对非酶糖基化反应的抑制率。20L发酵罐转化EGB最佳工艺条件为72h前通气量为8L/min,72h后为4.8L/min,转速为120r/min。     

HMM模型在检测复杂网络攻击中的应用

对于隐蔽性强、持续时间长且分步完成的复杂网络攻击,现有的入侵检测技术仍无法有效地进行识别。详细地分析了复杂网络攻击的特征,并在此基础上建立了复杂网络攻击的HMM检测模型。通过关联分析不同网络监视器的报警事件,产生用于HMM模型训练及检测的报警序列,这些报警序列本质上反映了攻击者的行为。实验结果表明,该模型能较好地检测复杂网络攻击。     

蒜薹四种MA贮藏保鲜袋的应用效果研究

用PE硅窗袋、PVC硅窗袋、SCA袋和定期换气袋贮存蒜薹240d,对袋内气体的变化规律和蒜薹的贮存质量进行评价。结果表明:前三种MA袋的气体指标稳定,定期换气袋内的气体呈不稳定的周期性变化。PVC硅窗袋和SCA硅窗袋贮存蒜薹的质量最好。PE硅窗袋次之,定期换气袋较差。     

5-Br-PADAP-H_2O_2分光光度法测定油品中微量钒

利用 5 -Br-PADAP -H2 O2 -V三元体系分光光度法测定油品中微量钒 ,考查了各种影响因素 ,得到测定的适宜条件。结果表明 ,此三元体系在酸性条件下能长时间稳定 ,以H3PO4作介质 ,乙二胺四乙酸钠和酒石酸作掩蔽剂可消除油品中常见离子的干扰。方法操作简单、灵敏度高、准确性好 ,适用于油品中微量钒的分析。     

基于安源红色文化的智能型文创产品设计研究

目的 针对智能技术在红色文创产品设计应用上的方式方法与红色文化在当代的价值转换,以实践案例为切入点,探索智能技术与红色文创产品结合的设计策略与方法,为红色文创产品与智能技术融合提供新思路。方法 以“声动安源”智能故事机文创产品为设计实践对象,指出安源红色文创产品的设计现状,并通过对智能红色文创产品设计策略的分析与方法进行总结,完成实践对象的技术运用与安源红色内涵的可视化设计转译。结果 得出了智能技术融入红色文创产品设计的策略与创新方法。结论 通过设计实践,验证了智能技术在红色文创产品设计上的可行性与实操性,智能红色文创产品设计不应局限于对智能技术的简单应用,更应关注红色文化与智能技术在融合创新、育人创新、传播创新、营销创新等方面的拓展深度,从而切实提高红色文化在新时代的有效传播与继承。     

地表水与地下水耦合模型研究进展

描述地表水与地下水文过程耦合概念,论述国内外具有代表性的流域水文模型与地下水模型耦合的3种类型及耦合方法,提出地表水与地下水耦合模型的主要特征及适应性,并对今后的发展方向进行了展望。     

绿芦笋自发气调贮藏保鲜袋的设计和适宜气体组成确定

以产品呼吸和薄膜渗气往一定条件下达到平衡时，能自发调节保鲜袋内O2和CO2浓度为依据，用改变薄膜厚度、面积、贮藏量的方法调节袋内气体组成。在大量试验数据基础上，用计算机回归设计的芦笋自发气调贮藏保鲜袋，能够使芦笋贮藏保持在3．2％～8．3％O2；3．5％～5．3％CO2的适宜气组成范围内，保鲜效果优良。     

100 t级上仰预应力锚索在积石峡水电站隧洞工程中的应用

上仰预应力锚索较下倾锚索在施工中存在内锚固段灌浆、锚墩头制安等突出问题和困难,在积石峡水电站导流洞与中孔泄洪洞交叉段100 t级上仰预应力锚索施工中,采用高强度低松弛钢绞线自由式预应力锚索、三管两次灌浆、钢结构墩头等工艺和结构,成功解决了上仰预应力锚索施工中存在的问题,取得了良好的效果,可供其他类似工程借鉴使用。     

RELAXATION BEHAVIOR AND THE APPLICATION OF INTEGRAL CONSTITUTIVE EQUATIONS TO WHEAT DOUGH

The small amplitude rheological properties of doughs of different compositions were measured using a dynamic rheometer. In the first study, the effect of water, flour, and added gluten on the dynamic properties was investigated. Frequency sweep was carried out in the range of 0. 1 to 100 s⁻¹ at an applied strain of 0. 1%. Storage (G') and loss (G") moduli for each composition were recorded. The storage modulus versus frequency plots were a series of parallel curves indicating that the moisture-to-flour ratio was the most important parameter. The loss modulus versus frequency plot showed a falling-off after a frequency of 40 s⁻¹. A plot of tan δ versus frequency indicated a more elastic dough at shorter time     

<i>In Silico</i> analysis and linking of metabolism-related genes with the immune landscape in head and neck squamous cell carcinoma

Metabolic reprogramming and immunologic suppression are two critical characteristics promoting the progression of head and neck squamous cell carcinoma (HNSCC). The integrative analysis of all the metabolism-related genes (MRGs) in HNSCC is lacking and the interaction between the metabolism and the immune characteristics also requires more exploration to uncover the potential mechanisms. Therefore, this study was designed to establish a prognostic signature based on all the MRGs in HNSCC. Genes of HNSCC samples were available from the TCGA and GEO databases while the MRGs were retrieved from a previous study. Ultimately 4 prognostic MRGs were selected to construct a model possessing robust prognostic value and accuracy in TCGA cohorts. The favorable reproducibility of this model was confirmed in validation cohorts from GEO databases. The risk score calculated by this model was an independent prognostic factor that further classified these HNSCC patients into high-/low-risk groups. GSEA analyses and somatic mutations indicated the low-risk group could activate several anti-tumor pathways and possessed lower TP53 mutation. The results of ESTIMATE, single-sample GSEA, CIBERSORT, and some immune-related molecules analyses suggested the low-risk group exhibited lower metabolic activities and higher immune characteristics. The Spearman correlation test implied most metabolic pathways with tumor-promoting function were negatively correlated with the immune activity, indicating a plausible approach of combining the anti-metabolism and the immunotherapy drugs in the high-risk group to enhance therapeutic effects than applied separately. In conclusion, this prognostic signature linking MRGs with the immune landscape could promote the individualized treatment for HNSCC patients.