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51.
Pure and rare earth doped gadolinium oxide (Gd2O3) waveguide films were prepared by a simple sol-gel process and dip-coating method. Structure of Gd2O3 films annealed at different temperature was investigated by X-ray diffraction and transmission electron microscopy. Oriented growth of (400) face of Gd2O3 has been observed when the films were deposited on amorphous substrate. The refractive index and thickness of films were determined by m-lines spectroscopy. The laser beam (λ= 632.8 nm) was coupled into the film by a prism coupler and the propagation length is about 3.5 cm. Luminescence properties of europium ions doped films were measured by waveguide fluorescence spectroscopy, which shows disordered environment for Eu^3 at 400℃. 相似文献
52.
Spriet C Trinel D Waharte F Deslee D Vandenbunder B Barbillat J Héliot L 《Microscopy research and technique》2007,70(2):85-94
Studies of proteins' interaction in cells by FRET can take benefit from two important photo-physical properties describing fluorescent proteins: fluorescence emission spectrum and fluorescence lifetime. These properties provide specific and complementary information about the tagged proteins and their environment. However, none of them taken individually can completely quantify the involved fluorophore characteristics due to their multiparametric dependency with molecular environment, experimental conditions, and interpretation complexity. A solution to get a better understanding of the biological process implied at the cellular level is to combine the spectral and temporal fluorescence data acquired simultaneously at every cell region under investigation. We present the SLiM-SPRC160, an original temporal/spectral acquisition system for simultaneous lifetime measurements in 16 spectral channels directly attached to the descanned port of a confocal microscope with two-photon excitation. It features improved light throughput, enabling low-level excitation and minimum invasivity in living cells studies. To guarantee a fairly good level of accuracy and reproducibility in the measurements of fluorescence lifetime and spectra on living cells, we propose a rigorous protocol for running experiments with this new equipment that preserves cell viability. The usefulness of SLiM approach for the precise determination of overlapping fluorophores is illustrated with the study of known solutions of rhodamine. Then, we describe reliable FRET experiments in imaging mode realized in living cells using this protocol. We also demonstrate the benefit of localized fluorescence spectrum-lifetime acquisitions for the dynamic study of fluorescent proteins. proteins. 相似文献
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54.
Polarized light microscopy (PLM) is used to image individual single-walled carbon nanotubes (SWNTs) suspended in air across
a slit opening. The imaging contrast relies on the strong optical anisotropy typical of SWNTs. We combine PLM with a tunable
light source to enable hyperspectral excitation spectroscopy and nanotube chirality assignment. Comparison with fluorescence
microscopy and spectroscopy confirms the assignment made with PLM. This represents a versatile new approach to imaging SWNTs
and related structures.
相似文献
55.
Ligand‐Free Synthesis of Aluminum‐Doped Zinc Oxide Nanocrystals and their Use as Optical Spacers in Color‐Tuned Highly Efficient Organic Solar Cells 下载免费PDF全文
Meriem Gaceur Sadok Ben Dkhil David Duché Fatima Bencheikh Jean‐Jacques Simon Ludovic Escoubas Mahdi Mansour Antonio Guerrero Germà Garcia‐Belmonte Xianjie Liu Mats Fahlman Walid Dachraoui Abdou Karim Diallo Christine Videlot‐Ackermann Olivier Margeat Jörg Ackermann 《Advanced functional materials》2016,26(2):243-253
The color of polymer solar cells using an opaque electrode is given by the reflected light, which depends on the composition and thickness of each layer of the device. Metal‐oxide‐based optical spacers are intensively studied in polymer solar cells aiming to optimize the light absorption. However, the low conductivity of materials such as ZnO and TiO2 limits the thickness of such optical spacers to tenths of nanometers. A novel synthesis route of cluster‐free Al‐doped ZnO (AZO) nanocrystals (NCs) is presented for solution processing of highly conductive layers without the need of temperature annealing, including thick optical spacers on top of polymer blends. The processing of 80 nm thick optical spacers based on AZO nanocrystal solutions on top of 200 nm thick polymer blend layer is demonstrated leading to improved photocurrent density of 17% compared to solar cells using standard active layers of 90 nm in combination with thin ZnO‐based optical spacers. These AZO NCs also open new opportunities for the processing of high‐efficiency color tuned solar cells. For the first time, it is shown that applying solution‐processed thick optical spacer with polymer blends of different thicknesses can process solar cells of similar efficiency over 7% but of different colors. 相似文献
56.
Daniella Cesar-Silva Filipe S. Pereira-Dutra Ana Lucia Moraes Giannini Cecília Jacques G. de Almeida 《International journal of molecular sciences》2022,23(9)
This review aims to describe and discuss the different functions of the endolysosomal system, from homeostasis to its vital role during viral infections. We will initially describe endolysosomal system’s main functions, presenting recent data on how its compartments are essential for host defense to explore later how SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) and other coronaviruses subvert these organelles for their benefit. It is clear that to succeed, pathogens’ evolution favored the establishment of ways to avoid, escape, or manipulate lysosomal function. The unavoidable coexistence with such an unfriendly milieu imposed on viruses the establishment of a vast array of strategies to make the most out of the invaded cell’s machinery to produce new viruses and maneuvers to escape the host’s defense system. 相似文献
57.
Derek Hodson Gila Hanna Jacques Désautels 《Canadian Journal of Science, Mathematics, & Technology Education》2013,13(1):1-4
Abstract This article investigates some of the specific features involved in accommodating the idea of actual infinity as it appears in set theory. It focuses on the conceptions of two individuals with sophisticated mathematics background, as manifested in their engagement with variations of a well-known paradox: the ping-pong ball conundrum. The APOS theory is used as a framework to interpret participants’ efforts to resolve the paradoxes. The cases discussed focus on how transfinite subtraction may be conceptualized, and they suggest that there is more to accommodating the idea of actual infinity than the ability to act on a completed object—rather, it is the manner in which objects are acted upon that is also significant. 相似文献
58.
Mohamad-Ali Tehfe Jacques Lalevée Sofia Telitel Jifu Sun Jianzhang Zhao Bernadette Graff Fabrice Morlet-Savary Jean-Pierre Fouassier 《Polymer》2012,53(14):2803-2808
Household LED bulbs and halogen lamps are used to promote the ring-opening photopolymerization of epoxides in the presence of a new series of iridium(III) complexes (IrCs) exhibiting enhanced visible light absorption properties through the introduction of a coumarin moiety. These latter complexes are used as catalyst photoinitiators in an oxidative cycle in combination with a silane and an iodonium salt. Remarkably, even under the selected very soft irradiations (e.g. light intensity lower than 2 mW/cm2), excellent polymerization profiles are obtained (conversions > 80%). The mechanisms are investigated by ESR and luminescence experiments. 相似文献
59.
60.
The welfare of food-producing animals is a focus of public debate in Europe. Political institutions, have introduced regulations based on scientific data. Meanwhile, the practices of producers and transformers were modified. Implementation of care practices is added to the goal of sustainable basic health of animals. Nevertheless urban consumers still look for the “naturalness” of living animals. A brief historical perspective introduces the building process of European regulations. A short list of Directories and Recommendations provides a clue on the complexity of resulting construct. Now, this complexity is calling for simplification of rules while practices should be compatible with professional constraints. Few selected examples are brought to illustrate how the concepts initially studied by scientists (welfare, pain, stress, “consciousness”/awareness) were integrated in regulations and implemented by producers and meat industry in order to simultaneously maintain the requirements for high quality and security standards. At the same time, free trade market constraints introduced new distortions, in particular those linked to the world demand for proteins. Indeed, the controversy about animal welfare, initially brought on ethical grounds, became a case for ongoing adjustements of EU policy, requesting to combine scientific knowledge on animals, consequent evolution in the representation of animals by urban consumers with the challenge of adaptation and implemention of regulations. 相似文献