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171.
This paper investigates optimum distributed storage techniques for data preservation, and eventual dissemination, in opportunistic heterogeneous wireless sensor networks where data collection is intermittent and exhibits spatio‐temporal randomness. The proposed techniques involve optimally sharing the sensor nodes' storage and properly handling the storage traffic such that the buffering capacity of the network approaches its total storage capacity with minimum energy. The paper develops an integer linear programming (ILP) model, analyses the emergence of storage traffic in the network, provides performance bounds, assesses performance sensitivities and develops quasi‐optimal decentralized heuristics that can reasonably handle the problem in a practical implementation. These include the Closest Availability (CA) and Storage Gradient (SG) heuristics whose performance is shown to be within only 10% and 6% of the dynamic optimum allocation, respectively. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   
172.
The synthesis of beta-1,3-glucan, the structural component of the yeast cell wall that gives shape to the cell, occurs at the plasma membrane and is the result of the activity of at least a two-component complex. Fks1p is the catalytic subunit directly responsible for the synthesis of beta-1,3-glucan, whilst the second subunit, Rho1p, has a GTP-dependent regulatory role (Yamochi et al., 1994). RHO1 has been characterized in Saccharomyces cerevisiae (Yamochi et al., 1994), and in several other fungal species. In this work, we have used degenerate oligonucleotides derived from the conserved regions of Rho1ps to isolate the RHO1 gene of Yarrowia lipolytica. The gene isolated in this way, which we have named YlRHO1, encodes a 204 amino acid protein that shows a high degree of homology with other Rho1ps. However, unlike S. cerevisiae, the ylrho1Delta disruptant strain in Y. lipolytica is viable, although it exhibits an increased sensitivity to Calcofluor white and Congo red. Also, YlRHO1 complements rho1 lethality in S. cerevisiae at both 28 degrees C and 37 degrees C. The complete sequence of YlRHO1 can be obtained from GenBank under Accession No. AF279915.  相似文献   
173.
In this work we have studied the disulphide-bound group of cell wall mannoproteins of Yarrowia lipolytica and Candida albicans. In the case of Y. lipolytica, SDS-PAGE analysis of the beta-mercaptoethanol-extracted material from the purified cell walls of the yeast form, showed the presence of a main polypeptide of 45 kDa and some minor bands in the 100-200 kDa range. This pattern of bands is similar to that obtained in identical extracts in Saccharomyces cerevisiae (Moukadiri et al., 1999), and besides, all these bands cross-react with an antibody raised against beta-mercaptoethanol-extracted material from the purified cell walls of S. cerevisiae, suggesting that the 45 kDa band could be the homologue of Pir4 of S. cerevisiae in Y. lipolytica. To confirm this possibility, the amino-terminal sequences of two internal regions of the 45 kDa protein were determined, and degenerate oligonucleotides were used to clone the gene. The gene isolated in this way codes a 286 amino acid polypeptide that shows homology with the Pir family of proteins of S. cerevisiae (Russo et al., 1992; Toh-e et al., 1993), accordingly we have named this gene YlPIR1. Disruption of YlPIR1 led to a slight increase in the resistance of the cells to calcofluor white, Congo red and zymolyase, but did not cause changes in cell morphology, growth rate or morphological transition.  相似文献   
174.
In the developing of an optimal operation schedule for dams and reservoirs, reservoir simulation is one of the critical steps that must be taken into consideration. For reservoirs to have more reliable and flexible optimization models, their simulations must be very accurate. However, a major problem with this simulation is the phenomenon of nonlinearity relationships that exist between some parameters of the reservoir. Some of the conventional methods use a linear approach in solving such problems thereby obtaining not very accurate simulation most especially at extreme values, and this greatly influences the efficiency of the model. One method that has been identified as a possible replacement for ANN and other common regression models currently in use for most analysis involving nonlinear cases in hydrology and water resources–related problems is the adaptive neuro-fuzzy inference system (ANFIS). The use of this method and two other different approaches of the ANN method, namely feedforward back-propagation neural network and radial basis function neural network, were adopted in the current study for the simulation of the relationships that exist between elevation, surface area and storage capacity at Langat reservoir system, Malaysia. Also, another model, auto regression (AR), was developed to compare the analysis of the proposed ANFIS and ANN models. The major revelation from this study is that the use of the proposed ANFIS model would ensure a more accurate simulation than the ANN and the classical AR models. The results obtained showed that the simulations obtained through ANFIS were actually more accurate than those of ANN and AR; it is thus concluded that the use of ANFIS method for simulation of reservoir behavior will give better predictions than the use of any new or existing regression models.  相似文献   
175.
The present study has numerically investigated the Moderate or Intense Low oxygen Dilution (MILD) combustion regime, combustion processes and NO formation characteristics of the highly CO-rich syngas counterflow nonpremixed flames. To realistically predict the flame properties of the highly CO-rich syngas, the chemistry is represented by the modified GRI 3.0 mechanism. Computations are performed to precisely analyze the flame structure, NO formation rate, and EINO of each NO sub-mechanism. Numerical results reveal that the hydrogen enrichment and oxygen augmentation substantially influence the NO emission characteristics and the dominant NO production route in the CO-rich syngas nonpremixed flames under MILD and high temperature combustion regimes. It is found that the most dominant NO production routes are the NNH path for the lowest oxygen level (3%) and the thermal mechanism for the highest O2 condition (21%). For the intermediate oxygen level (9%), the most dominant NO production routes are the NNH route for the hydrogen fraction up to 5%, the CO2 path for the hydrogen fraction range from 5% to 10% and the thermal mechanism for the hydrogen fraction higher than 10%, respectively. To evaluate the contribution of the specific reaction on EINO the sensitivity coefficients are precisely analyzed for NO formation processes with the dominance of NNH/CO2/Thermal mechanism under the highly CO-rich syngas flames.  相似文献   
176.
177.
In recent years, there has been rapid and significant development of road transport technologies in order to reduce the number of killed and injured people on roads. These include safety technologies, emergency call systems (eCall), and advanced traveler information systems. The eCall system is an automatic in‐vehicle emergency call service, which is mainly used for notifying emergency services about dangerous road situations and their exact location. In this paper, an eCall platform prototype is developed to allow quick and efficient rescue of injured people in dangerous road situations. The eCall function is developed and installed in nomadic devices (e.g., smartphones and tablets). Large‐scale field operational tests were conducted in real settings to assess the impacts of the eCall function provided by in‐vehicle nomadic devices. More precisely, experiments were conducted by more than 250 participants with different sociodemographic profiles in order to study the users' acceptance of the use of the developed eCall function for large‐scale usage. The collected data are analyzed, and results are reported. Performed experiments showed the usefulness, acceptance, and satisfactory performance of the eCall service. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   
178.
In this study, a nonlinear conical spring bracing (NCSB) system that can be applied as a lateral resistance component in framed structures was developed to mitigate the vibration effects of earthquake and wind. The NCSB device consists of two solid telescopic conical springs attached to steel wire ropes. The application of NCSB in framed structures, particularly moment-resisting steel frame (MRSF), improves the seismic behavior of the frame because of the variable action of the NCSB device. NCSB stiffness is not considerable in the low to medium vibration range compared with structural stiffness. Therefore, the inherent ductility of MRSF is unaffected because of the addition of the NCSB device to the frame. However, with its large displacement value, NCSB stiffness increases and prevents excessive displacement in structures. A mathematical model of the NCSB device that considers the effect of cable stiffness is developed and implemented in program code. Furthermore, the seismic behavior of eight types of NCSB applications in frames subjected to different earthquake accelerations is evaluated in terms of displacement, velocity, and acceleration, as well as compared with bare and brace frames. Results reveal the reduction influences of the NCSB device on framed structures. The best geometric configuration for the NCSB system is also determined by using the proposed numerical analysis.  相似文献   
179.
User cooperation has evolved as a popular coding technique in wireless relay networks (WRNs). Using the neighboring nodes as relays to establish a communication between a source and a destination achieves an increase of the diversity order. The relay nodes can be seen as a distributed multi‐antenna system, which can be exploited for transmit diversity by using distributed space–time block coding (STBC). In this paper, we investigate the bit error rate (BER) of multi‐hop WRNs employing distributed STBC at the relay nodes. We develop the general model of WRNs using distributed STBC, and we derive the pairwise error probability and an approximation of the BER. We examine the impact of several parameters, such as distributed STBC at the relays, the number of relays, the distances between the nodes, and the channel state information available at the receivers, on the BER performance of the multi‐hop WRN. The obtained results provide guidelines about the expected error performance and the design of channel estimation for these networks. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   
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