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Andrea Nolte Susanne Hossfeld Marcell Post Jan Niederlaender Tobias Walker Christian Schlensak Hans Peter Wendel 《Journal of materials science. Materials in medicine》2014,25(9):2121-2130
Chitosan is used in several pharmaceutical and medical applications, owing to its good cytocompatibility and hemocompatibility. However, there are conflicting reports regarding the biological activities of chitosan with some studies reporting anti-inflammatory properties while others report pro-inflammatory properties. In this regards we analyzed the endotoxin content in five different chitosans and examined these chitosans with their different deacetylation degrees for their hemocompatibility and cytocompatibility. Therefore, we incubated primary human endothelial cells or whole blood with different chitosan concentrations and studied the protein and mRNA expression of different inflammatory markers or cytokines. Our data indicate a correlation of the endotoxin content and cytokine up-regulation in whole blood for Poly-Morpho-Nuclear (PMN)-Elastase, soluble terminal complement complex SC5b-9, complement component C5/C5a, granulocyte colony-stimulating factor, Interleukin-8 (IL), IL-10, IL-13, IL-17E, Il-32α and monocyte chemotactic protein-1. In contrast, the incubation of low endotoxin containing chitosans with primary endothelial cells resulted in increased expression of E-selectin, intercellular adhesion molecule-1, vascular cell adhesion protein-1, IL-1β, IL-6 and IL-8 in endothelial cells. We suggest that the endotoxin content in chitosan plays a major role in the biological activity of chitosan. Therefore, we strongly recommend analysis of the endotoxin concentration in chitosan, before further determining if it has pro- or anti-inflammatory properties or if it is applicable for pharmaceutical and medical fields. 相似文献
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Jörn Reinders Robert Sonntag Jan Philippe Kretzer 《Journal of materials science. Materials in medicine》2014,25(11):2463-2469
Polyethylene wear (PE) is known to be a limiting factor in total joint replacements. However, a standardized wear test (e.g. ISO standard) can only replicate the complex in vivo loading condition in a simplified form. In this study, two different parameters were analyzed: (a) Bovine serum, as a substitute for synovial fluid, is typically replaced every 500,000 cycles. However, a continuous regeneration takes place in vivo. How does serum-replacement interval affect the wear rate of total knee replacements? (b) Patients with an artificial joint show reduced gait frequencies compared to standardized testing. What is the influence of a reduced frequency? Three knee wear tests were run: (a) reference test (ISO), (b) testing with a shortened lubricant replacement interval, (c) testing with reduced frequency. The wear behavior was determined based on gravimetric measurements and wear particle analysis. The results showed that the reduced test frequency only had a small effect on wear behavior. Testing with 1 Hz frequency is therefore a valid method for wear testing. However, testing with a shortened replacement interval nearly doubled the wear rate. Wear particle analysis revealed only small differences in wear particle size between the different tests. Wear particles were not linearly released within one replacement interval. The ISO standard should be revised to address the marked effects of lubricant replacement interval on wear rate. 相似文献
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Jan Berger Evan J. Spadafora Pingo Mutombo Pavel Jelínek Martin Švec 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(30):3686-3693
A single atomic manipulation on the delta‐doped B:Si(111)‐()R30° surface using a low temperature dynamic atomic force microscopy based on the Kolibri sensor is investigated. Through a controlled vertical displacement of the probe, a single Si adatom in order to open a vacancy is removed. It is shown that this process is completely reversible, by accurately placing a Si atom back into the vacancy site. In addition, density functional theory simulations are carried out to understand the underlying mechanism of the atomic manipulation in detail. This process also rearranges the atoms at the tip apex, which can be effectively sharpened in this way. Such sharper tips allow for a deeper look into the Si adatom vacancy site. Namely, high‐resolution images of the vacancy showing subsurface Si dangling bond triplets, which surround the substitutional B dopant atom in the first bilayer, are achieved. 相似文献
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Jan Herrmann Jürgen KoreckMatthias Maess Lothar Gaul 《Mechanical Systems and Signal Processing》2011,25(3):981-990
The integration of a model for longitudinal hydroacoustic fluid damping in thin hydraulic pipes in 3D finite element models is presented in this paper. In order to perform quantitative prediction of the vibroacoustic behavior and resulting noise levels of such fluid-structure coupled system due to hydraulic excitation, an accurate frequency-dependent fluid damping model including friction effects near the pipe wall is required. This step is achieved by matching complex wave numbers from analytical derivation into a parameterized damped wave equation and consecutive translation into finite element modeling.Since the friction effect close to the pipe wall changes locally with the inner pipe radius, the fluid damping model is applied segment-wise in order to model the influence of cross-sectional discontinuity, such as orifices, on the oscillating pressure pulsations. A component synthesis approach, which uses pipe segments as substructures, allows a simple model generation and fast computation times.The numerical harmonic results are compared to experimental frequency response functions, which are performed on a hydraulic test bench driven by a dynamic pressure source in the kHz-range. 相似文献