Reprint of: Improved cytotoxicity testing of magnesium materials

Metallic magnesium (Mg) and its alloys are highly suitable for medical applications as biocompatible and biodegradable implant materials. Magnesium has mechanical properties similar to bone, stimulates bone regeneration, is an essential non-toxic element for the human body and degrades completely within the body environment. In consequence, magnesium is a promising candidate as implant material for orthopaedic applications. Protocols using the guideline of current ISO standards should be carefully evaluated when applying them for the characterization of the cytotoxic potential of degradable magnesium materials. For as-cast material we recommend using 10 times more extraction medium than recommended by the ISO standards to obtain reasonable results for reliable cytotoxicity rankings of degradable materials in vitro. In addition primary isolated human osteoblasts or mesenchymal stem cells should be used to test magnesium materials.     

EPA's Fracking Fluids Report Could Help Stop Negative State Moves

For much of the 20th century, domestic oil and gas development regulation was left up to the states. In doing so, this policy ensured that, depending on the state's specific interests, each could pass regulations that it deemed appropriate to protect the environment while simultaneously bolstering oil and gas production. Although management has primarily remained in the hands of the states, the federal government is still very involved in the industry, passing public health–oriented regulations such as the Safe Drinking Water Act (SDWA) and the Clean Water Act (CWA), and administering such regulatory bodies as the Occupational Safety and Health Administration (OSHA) to oversee production and recovery operations. For example, in constructing a well for the use of secondary recovery processes, oil and gas companies are subject to the CWA and oversight by OSHA, as well as Superfund regulations and the SDWA in the production phase.     

Causal Effects of Aroma Compounds on Royal Gala Apple Flavours

Volatile flavour compounds produced by Royal Gala apple have been identified by GC-MS. Major components were 2-methylbutyl acetate, butyl acetate, hexyl acetate, butanol, 2-methylbutanol and hexanol. Odour-port evaluation of the components separated by GC indicated that the first four compounds were important contributors to the aroma and flavour. Use of analytical sensory panels revealed that 2-methylbutyl acetate, butanol and hexyl acetate had the greatest causal effect on those aroma and flavour attributes considered important for Royal Gala apples.     

Comparison of the relative recovery of polyphenolics in two fruit extracts from a model of degradation during digestion and metabolism

To simulate the effects of digestion and metabolism on the survival of different polyphenolic compounds, extracts of blueberry and apple were deglycosylated by acid hydrolysis, followed by enzymic glucuronidation under neutral conditions, yielding approximately 5% overall recovery of polyphenolics. The major polyphenolics before and after the treatment were compared, to estimate which species are likely to be present in the intestinal lumen, undegraded and available for absorption, after consumption of the fruit. Whereas blueberry extract consisted predominantly of anthocyanins, epicatechin and caffeoyl quinate esters, the major components of the treated extract were quercetin glucuronides and (unglucuronidated) caffeoyl quinates, with only traces of anthocyanidin derivatives. In apple extract, compositional changes were less marked, but caffeoyl quinates, procyanidins and quercetin were enriched at the expense of caffeic acid, epicatechin and catechin. Hydrophobic compounds like phloretin and quercetin were extensively glucuronidated, whereas caffeic acid and caffeoyl quinate were not. These results suggest that the major polyphenolic components of a fruit are not necessarily the most important contributors to any health benefits because the polyphenolic composition in the intestinal lumen and consequently, in the circulation, may be considerably different.     

A comparative study of composition and postharvest performance of organically and conventionally grown kiwifruits

Postharvest performance of organic and conventional ‘Hayward’ kiwifruits grown on the same farm in Marysville, California, and harvested at the same maturity stage were compared in this study. Quality parameters monitored included morphological (shape index) and physical (peel characteristics) attributes of the initial samples. Maturity indices (CO₂ and C₂H₄ production, firmness, color, soluble solids content and acidity) and content of compounds associated with flavor and nutritional quality (minerals, sugars and organic acids, ascorbic acid, total phenolics, and antioxidant activity) were determined at 0, 35, 72, 90 and 120 days of storage at 0°C, and after 1 week of shelf‐life simulation at 20°C, after each storage duration. Organically and conventionally grown kiwifruits had similar soluble solids content at harvest, but conventional kiwifruits had a higher firmness and L* value, and a lower hue angle and chromaticity, resulting in a lighter green color when compared with the organic kiwifruits. These differences were maintained for all the storage durations, with the soluble solids content increasing more in conventionally grown kiwifruits. The two production systems resulted in different morphological attributes since organic kiwifruits exhibited a larger total and columella area, smaller flesh area, more spherical shape, and thicker skin compared to conventional kiwifruits. All the main mineral constituents were more concentrated in organic kiwifruits, which also had higher levels of ascorbic acid and total phenol content, resulting in a higher antioxidant activity. Sugars and organic acids composition was not affected by the production system. Copyright © 2007 Society of Chemical Industry     

Deformation and water loss from solvent filled microcapsules under compressive loads

Microcapsules filled with liquid solvents for CO₂ absorption can be easily deformed due to their elastic polymer shells. We present a combination of experiments and model predictions to demonstrate that modest compressive forces can lead to significant capsule deformation and performance issues for this enabling technology. Contrary to expectations based on Raoult's law, capsules containing aqueous carbonate solution were found to lose water to flows of humidified nitrogen in centimeter-scale packed beds. Water loss increased with gas velocity, suggesting compression was responsible for mass transfer, an interpretation supported by microscope images of deformed and broken capsules. A model for compression induced mass transfer under packed/fluidized bed operating conditions was developed and validated with the experimental data for a range of conditions (gas velocities, temperatures, humidities). Design criteria for future generations of microcapsules that will more effectively resist compression are evaluated.     

Synthesis and Reactivity of Iron(II) Complexes with a New Tripodal Imine Ligand

A new tripodal imine ligand tris(2-(propan-2-ylideneamino)ethyl)amine (imine₃tren) was prepared in order to stabilize high valent iron-oxido complexes. Iron complexes were synthesized in template reactions from iron(II) salts, tris(2-aminoethyl)amine (tren) and acetone. Due to the reversibility of the imine formation, complexes with different ligands were obtained depending on the reaction conditions. Three complexes, [Fe(imine₃tren)(OAc)₂] ( 1 ), [Fe(imine₃tren)(OAc)]OTf ( 2 ) and [(imine₃tren)₂Fe₂(F)₂](SbF₆)₂ ( 3 ), could be synthesized and structurally characterized. However, reactions with hydrogen peroxide, iodosobenzene or ozone did not lead to any kind of “oxygen adduct” complex that could be spectroscopically observed.     

Fate of fertilizer 15N in intensive ridge cultivation with plastic mulching under a monsoon climate

Reducing nitrogen (N) leaching to groundwater requires an improved understanding of the effect of microtopography on N fate. Because of the heterogeneity between positions, ridge tilled fields, frequently used in intensive agriculture, should be treated as two distinct management units. In this study, we measured N dynamics in plastic-mulched ridges and bare furrows with the goal of developing more sustainable agricultural practices with optimal gains, namely crop production versus limited impacts on water quality. We investigated: (1) biomass production; (2) crop N uptake; (3) N retention in soil; and (4) N leaching using ¹⁵N fertilizer in a radish crop. Broadcast mineral N fertilizer application prior to planting resulted in high total leaching losses (of up to 390 N kg ha^?1). The application of plastic mulch in combination with local fertilizer management did not help to reduce N leaching. At all fertilizer N rates, the mean NO₃ ^? concentrations in seepage water were found to be above the WHO drinking water standard of 50 mg NO₃ ^? l^?1. To reduce NO₃ ^? leaching, we recommend: (1) decreasing the fertilizer N rates to a maximum of 150 kg N ha^?1; (2) applying fertilizer N in 3–4 split applications according to the plant’s N needs; (3) applying fertilizer N to the ridges (after their formation) to avoid losses from the furrows; and (4) increasing the soil organic matter content to enhance the water and nutrient retention by covering the furrows with plant residues.     

Target Score—A Proteomics Data Selection Tool Applied to Esophageal Cancer Identifies GLUT1-Sialyl Tn Glycoforms as Biomarkers of Cancer Aggressiveness

Esophageal cancer (EC) is a life-threatening disease, demanding the discovery of new biomarkers and molecular targets for precision oncology. Aberrantly glycosylated proteins hold tremendous potential towards this objective. In the current study, a series of esophageal squamous cell carcinomas (ESCC) and EC-derived circulating tumor cells (CTCs) were screened by immunoassays for the sialyl-Tn (STn) antigen, a glycan rarely expressed in healthy tissues and widely observed in aggressive gastrointestinal cancers. An ESCC cell model was glycoengineered to express STn and characterized in relation to cell proliferation and invasion in vitro. STn was found to be widely present in ESCC (70% of tumors) and in CTCs in 20% of patients, being associated with general recurrence and reduced survival. Furthermore, STn expression in ESCC cells increased invasion in vitro, while reducing cancer cells proliferation. In parallel, an ESCC mass spectrometry-based proteomics dataset, obtained from the PRIDE database, was comprehensively interrogated for abnormally glycosylated proteins. Data integration with the Target Score, an algorithm developed in-house, pinpointed the glucose transporter type 1 (GLUT1) as a biomarker of poor prognosis. GLUT1-STn glycoproteoforms were latter identified in tumor tissues in patients facing worst prognosis. Furthermore, healthy human tissues analysis suggested that STn glycosylation provided cancer specificity to GLUT1. In conclusion, STn is a biomarker of worst prognosis in EC and GLUT1-STn glycoforms may be used to increase its specificity on the stratification and targeting of aggressive ESCC forms.