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101.
Magnetic resonance tagging has proven useful in the visualization and quantification of cardiac motion. Traditionally, tags are designed to have crisp geometric profiles in order to enhance both visualization and detection of tags. Recent image acquisition and analysis methods, however, have been designed to exploit sinusoidal tag profiles. This paper presents a method based on harmonic phase (HARP) concepts to synthesize tag lines that have both crisp profiles and alternative orientations from the original sinusoidal patterns. Results are demonstrated on images acquired with SPAMM, CSPAMM, and fast-HARP pulse sequences. 相似文献
102.
Davidson W McGibbon GA White PW Yoakim C Hopkins JL Guse I Hambly DM Frego L Ogilvie WW Lavallée P Archambault J 《Analytical chemistry》2004,76(7):2095-2102
An indandione-containing class of inhibitors abrogates DNA replication of human papillomavirus (HPV) types 6 and 11 by binding reversibly to the transactivation domain (TAD) of the viral E2 protein and inhibiting its interaction with the viral E1 helicase. To locate the binding site of this class of protein-protein interaction inhibitors, a benzophenone derivative was used to generate an irreversibly labeled E2-TAD polypeptide. The single site of covalent modification of the E2-TAD was identified by proteolytic digestions using trypsin, LysC, and V8 proteases and characterization of the resulting peptides by LC-MS procedures. Through this methodology, the benzophenone attachment point was located at the terminal methyl of residue Met101. Evidence further pinpointed the site of photoaffinity attachment to the terminal carbon atom, which is significant in providing a definitive example of the ability to locate photoinduced cross-linking to a polypeptide with atomic resolution using solely mass spectrometric detection. The location of the inhibitor binding site vis-à-vis the Glu39 and Glu100 residues sensitive to mutation for HPV 11 E2-TAD is discussed in relation to the crystal structure of the E2-TAD from the related HPV type 16. 相似文献
103.
William Banks John L. Clapperton Morag E. Kelly Agnes G. Wilson Robert J. M. Crawford 《Journal of the science of food and agriculture》1980,31(4):368-374
The presence in the bovine mammary gland of a desaturase specific for the conversion of stearic to oleic acid allows the manipulation of the physical properties of milk fat by varying the dietary intake of C16 and C18 fatty acids. In particular, feeding the dairy cow an oil with a high C18: C16 ratio should allow the proportion of milk fat that is liquid at 5°C to be increased, with a consequent improvement in the low-temperature spreadability of butter. In this paper, the effect of feeding concentrate rations containing (a) soya oil as the free oil and as cracked soya beans; (b) various levels of soya oil, the ration being offered twice or 24-times daily, on the thermal properties of the milk fat and on the yield of milk constituents is examined in detail. Feeding ratios containing free soya oil greatly increased the proportion of milk fat liquid at 5°C, whereas cracked soya oil gave only slight increases. Proportion and yield of milk fat were decreased by feeding soya oil twice daily but were increased by continuous feeding. 相似文献
104.
The binary phase diagram of the SrF2-MgF2 system below 1200°C has been constructed from DTA cooling data. The system shows an eutectic at 48.4 mole % MgF2 and 871°C, and a peritectic at 50 mole % and 880°C, corresponding to the compound SrMgF4, previously prepared here by solid state reaction. A solid-solid transition in SrMgF4 occurs at about 809°C. Small single crystals of SrMgF4 were obtained by extremely slow cooling of a stoichiometric melt. 相似文献
105.
Lukasik J Bradley ML Scott TM Dea M Koo A Hsu WY Bartz JA Farrah SR 《Journal of food protection》2003,66(2):188-193
The efficacy levels of different physical and chemical washing treatments in the reduction of viral and bacterial pathogens from inoculated strawberries were evaluated. Escherichia coli O157:H7, Salmonella Montevideo, poliovirus 1, and the bacteriophages PRD1, phiX174, and MS2 were used as model and surrogate organisms. Chemicals readily available to producers and/or consumers were evaluated as antimicrobial additives for the production of washes. The gentle agitation of contaminated strawberries in water for 2 min led to reductions in microbial populations ranging from 41 to 79% and from 62 to 90% at water temperatures of 22 and 43 degrees C, respectively. Significant reductions (> 98%) in numbers of bacteria and viruses were obtained with sodium hypochlorite (50 to 300 ppm of free chlorine), Oxine or Carnebon (200 ppm of product generating "stabilized chlorine dioxide"), Tsunami (100 ppm of peroxyacetic acid), and Alcide (100 or 200 ppm of acidified sodium chlorite) washes. Overall, 200 ppm of acidified sodium chlorite produced the greatest reductions of microorganisms. Hydrogen peroxide (0.5%) was slightly less effective than free chlorine in a strawberry wash and caused slight fruit discoloration. Cetylpyridinium chloride (0.1%) was effective in the reduction of bacterial species, while trisodium phosphate (1%) was effective against viruses. The consumer-oriented produce wash Fit was very effective (> 99%) in reducing the numbers of bacteria but not in reducing the numbers of viruses. Another wash, Healthy Harvest, was significantly less effective than Fit in reducing bacterial pathogens but more effective for viruses. The performance of automatic dishwashing detergent was similar to that of Healthy Harvest and significantly better than that of liquid dishwashing detergent. Solutions containing table salt (2% NaCl) or vinegar (10%) reduced the numbers of bacteria by about 90%, whereas only the vinegar wash reduced the numbers of viruses significantly (ca. 95%). 相似文献
106.
We outline an Eulerian framework for computing the thickness of tissues between two simply connected boundaries that does not require landmark points or parameterizations of either boundary. Thickness is defined as the length of correspondence trajectories, which run from one tissue boundary to the other, and which follow a smooth vector field constructed in the region between the boundaries. A pair of partial differential equations (PDEs) that are guided by this vector field are then solved over this region, and the sum of their solutions yields the thickness of the tissue region. Unlike other approaches, this approach does not require explicit construction of any correspondence trajectories. An efficient, stable, and computationally fast solution to these PDEs is found by careful selection of finite differences according to an upwinding condition. The behavior and performance of our method is demonstrated on two simulations and two magnetic resonance imaging data sets in two and three dimensions. These experiments reveal very good performance and show strong potential for application in tissue thickness visualization and quantification. 相似文献
107.
An approach for the characterization of glycosylation sites and oligosaccharide heterogeneity in glycoproteins based on a combination of nonspecific proteolysis, deglycosylation, and matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FT MS) is described. Glycoproteins were digested with Pronase yielding primarily glycopeptides and amino acids. Nonglycosylated peptide fragments were susceptible to complete Pronase digestion to their constituent amino acids. Steric hindrance prohibited the digestion of the peptide moiety attached to the glycan. Glycopeptides were desalted and concentrated using solid-phase extraction and analyzed by MALDI MS. The oligosaccharides were also analyzed by MALDI MS after releasing the glycans from glycoproteins using PNGase F. The peptide moiety of the glycopeptides was identified by subtracting the masses of the glycans derived from PNGase F treatment from the masses of the glycopeptides. The experimental strategy was validated using glycoproteins with known oligosaccharide structures, ribonuclease B and chicken ovalbumin. This procedure was then used to determine the N-glycosylation sites and site heterogeneity of a glycoprotein whose glycosylation pattern was unknown, namely, the Xenopus laevis egg cortical granule lectin. This procedure is useful for determining protein site heterogeneity and structural heterogeneities of the oligosaccharide moiety of glycoproteins. 相似文献
108.
Fibre-optic components fabricated on the same substrate as integrated circuits are important for future high-speed communications. One industry response has been the costly push to develop indium phosphide (InP) electronics. However, for fabrication simplicity, reliability and cost, gallium arsenide (GaAs) remains the established technology for integrated optoelectronics. Unfortunately, the GaAs bandgap wavelength (0.85 microm) is far too short for fibre optics at 1.3-1.5 microm. This has led to work on materials that have a large lattice mismatch on GaAs. Here we demonstrate the first light-emitting diode (LED) that emits at 1.5 microm fibre-optic wavelengths in GaAs using optical transitions from arsenic antisite (As(Ga)) deep levels. This is an enabling technology for fibre-optic components that are lattice-matched to GaAs integrated circuits. We present experimental results showing significant internal optical power (24 mW) and speed (in terahertz) from GaAs optical emitters using deep-level transitions. Finally, we present theory showing the ultimate limit to the efficiency-bandwidth product of semiconductor deep-level optical emitters. 相似文献
109.
Mandeep M. Sahani Tarek M. Daoud Ramin Sam Jerry Andrews Yuk Lun Cheng Carl M. Kjellstrand Todd S. Ing 《Hemodialysis international. International Symposium on Home Hemodialysis》2001,5(1):92-96
An aggressive dialysis in a grossly azotemic patient, especially one with severe metabolic acidosis, can lead to dialysis disequilibrium syndrome (DDS). Mild forms present as nausea, vomiting, restlessness, and headache. Severe manifestations include seizures, obtundation, coma, and even death. This clinical picture is caused by cerebral edema induced by one or more of the following mechanisms:
- 相似文献
110.
Roberto Passerone Jerry R. Burch Alberto L. Sangiovanni-Vincentelli 《Formal Methods in System Design》2007,31(1):1-33
Embedded systems are electronic devices that function in the context of a real environment, by sensing and reacting to a set
of stimuli. Because of their close interaction with the environment, and to simplify their design, different parts of an embedded
system are best described using different notations and different techniques. In this case, we say that the system is heterogeneous. We informally refer to the notation and the rules that are used to specify and verify the elements of heterogeneous systems
and their collective behavior as a model of computation. In this paper, we consider different classes of relationships between models of computation and discuss their preservation
properties with respect to the model's refinement relation and composition operator. In particular, we focus on abstraction
and refinement relationships in the form of abstract interpretations and introduce the notion of conservative approximation. We show that, unlike abstract interpretations, conservative approximations preserve refinement verification results from
an abstract to a concrete model while avoiding false positives. We also characterize the relationship between abstract interpretations
and conservative approximations, and derive necessary and sufficient conditions to obtain a conservative approximation from
a pair of abstract interpretations. In addition, we use the inverse of a conservative approximation to identify components
that can be used indifferently in several models, thus enabling reuse across models of computation. The concepts described
in this paper are illustrated with examples from continuous time and discrete time models of computation. 相似文献