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排序方式: 共有1137条查询结果,搜索用时 15 毫秒
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Joanna Szpotkowska Kamil Szpotkowski Jerzy Ciesioka 《International journal of molecular sciences》2022,23(17)
A mouse model has often been used in studies of p53 gene expression. Detailed interpretation of functional studies is, however, hampered by insufficient knowledge of the impact of mouse p53 mRNA’s structure and its interactions with proteins in the translation process. In particular, the 5′-terminal region of mouse p53 mRNA is an important region which takes part in the regulation of the synthesis of p53 protein and its N-truncated isoform Δ41p53. In this work, the spatial folding of the 5′-terminal region of mouse p53 mRNA and its selected sub-fragments was proposed based on the results of the SAXS method and the RNAComposer program. Subsequently, RNA-assisted affinity chromatography was used to identify proteins present in mouse fibroblast cell lysates that are able to bind the RNA oligomer, which corresponds to the 5′-terminal region of mouse p53 mRNA. Possible sites to which the selected, identified proteins can bind were proposed. Interestingly, most of these binding sites coincide with the sites determined as accessible to hybridization of complementary oligonucleotides. Finally, the high binding affinity of hnRNP K and PCBP2 to the 5′-terminal region of mouse p53 mRNA was confirmed and their possible binding sites were proposed. 相似文献
33.
Steven J. Cunning Jerzy W. Rozenblit 《Journal of Intelligent and Robotic Systems》2005,41(2-3):87-112
A method for the automatic generation of test scenarios from the behavioral requirements of a system is presented in this paper. The generated suite of test scenarios validates the system design or implementation against the requirements. The approach proposed here uses a requirements model and a set of four algorithms. The requirements model is an executable model of the proposed system defined in a deterministic state-based modeling formalism. Each action in the requirements model that changes the state of the model is identified with a unique requirement identifier. The scenario generation algorithms perform controlled simulations of the requirements model in order to generate a suite of test scenarios applicable for black box testing. Measurements of several metrics on the scenario generation algorithms have been collected using prototype tools. 相似文献
34.
The study of chemical composition and dough rheology changes in sieve‐classified two fractions (up to 60 and 60–240 μm particles) of wheat flour was the subject of this study. The straight grade flours were obtained by the milling of three Polish varieties of spring wheat, differing in ?? abbreviation? (PSI) values. The flours were separated with the use of a SZ‐1 laboratory sifter. The yield of fine fraction was in the range 50.0–55.7%. The obtained fractions were assayed for the content and composition of free lipids, gluten proteins, damaged starch, ash, water absorption and amylograph viscosity. Dough rheology (extrusion in OTMS cell, alveograph and farinograph tests) and baking trials were also performed. The content of free lipids, including the non‐polar and phospholipids was lower and the content of glycolipids was higher in fine flours. Those fractions were more rich in linoleic acid but the lower content of oleic and linolenic acids resulted in a higher oxidizability index of free lipids. Fine flours contained less ash and significantly more damaged starch. At the same time, they were characterized by a higher content of wet gluten, water absorption, amylograph viscosity and better dough parameters. This was reflected in the bread volume, which was higher by 6.3–10.7%. The influence of the changes in composition and the content of free lipids upon the rheology of the dough after the 90 days flour storage has not been defined unambiguously and requires further research. 相似文献
35.
Patrick G. Clark Jerzy W. Grzymala-Busse Wojciech Rzasa 《Journal of Intelligent Information Systems》2016,47(3):515-529
A probabilistic approximation is a generalization of the standard idea of lower and upper approximations, defined for equivalence relations. Recently probabilistic approximations were additionally generalized to an arbitrary binary relation so that probabilistic approximations may be applied for incomplete data. We discuss two ways to induce rules from incomplete data using probabilistic approximations, by applying true MLEM2 algorithm and an emulated MLEM2 algorithm. In this paper we report novel research on a comparison of both approaches: new results of experiments on incomplete data with three interpretations of missing attribute values. Our results show that both approaches do not differ much. 相似文献
36.
Applications of fuzzy‐based linguistic patterns for the assessment of computer screen design quality
Jerzy Grobelny Waldemar Karwowski Jozef Zurada 《International journal of human-computer interaction》2013,29(3):193-212
The main objective of this study was to develop a modeling framework which would unify different aspects of computer screen design and result in a quantitative criterion for an optimized computer screen format. The fuzzy set‐based linguistic design patterns were utilized as a tool to build this model. The linguistic patterns are based on categories of expressions related closely to natural language and truth values, which are close to a human designer's intuition. The proposed framework is capable of assessing the quality of computer screen design based on existing knowledge in human‐computer interface domain using the fuzzy‐based linguistic pattern approach. Exemplary patterns for an optimal screen density, information grouping, and some aspects of screen layout are presented, along with a sequence of calculations based on the exemplary screen format. This study showed that it is possible to achieve a rational and relatively easy to interpret assessment of different screen designs in the form of the degrees of truth. Such an evaluation criterion reflects the compatibility of a given screen design with the optimal one based on the current knowledge in the field. It is believed that the proposed methodological framework for computer screen design should significantly augment the efforts of human designers. 相似文献
37.
Jerzy Jedlinski Jean Luc Grosseau Poussard Jarosław Dabek Kazimierz Kowalski Marek Nocun Grzegorz Smola Zbigniew Żurek 《Oxidation of Metals》2017,88(3-4):371-382
The very early stages of the oxidation of an Fe20Cr2Al alloy, unmodified and ion-implanted by aluminium, yttrium and a combination of both elements, Al and Y, were studied at 1100 °C in oxygen using two-stage-oxidation exposures with 18O2 as a tracer and subsequent characterisation of the scales using SIMS analyses of distribution of oxygen isotopes and oxide-related negative ion clusters, SEM observations of the surface morphology and photoluminescence spectroscopy analysis of the phase composition. The scales formed in all cases, except for the Al-implanted alloy, exhibited layered structures, with the outer part comprising Fe- and Cr-rich oxide, and the inner part being Al2O3, which grew due to a mixed outward–inward mechanism . The alumina sub-layers contained the transient oxides and α-Al2O3. Implanted Al significantly affected the mechanism of the scale growth, providing that the scale consisted essentially of α-Al2O3, and grew via a mixed inward-outward mechanism typical for scales on alumina formers. 相似文献
38.
Ewa Watorek Tomasz Golebiowski Mariusz Kusztal Krzysztof Letachowicz Waldemar Letachowicz Hanna Augustyniak Bartosik Jerzy Garcarek Katarzyna Madziarska Wacław Weyde Marian Klinger 《Hemodialysis international. International Symposium on Home Hemodialysis》2014,18(1):113-117
Elderly patients, defined as octogenarians and nonagenarians, are an increasing population entering renal replacement therapy. Advanced age appears as an exclusive factor negatively influencing dialysis practice. Elderly patients are referred late for the initiation of hemodialysis and more likely are offered catheters rather than arteriovenous fistulae (AVF), which increase mortality and negatively affect quality of life. We present our approach to the creation of vascular access for hemodialysis in this demanding population. In 2006–2012, 39 patients aged 85.9 ± 2.05 with end‐stage renal disease, mainly resulting from ischemic nephropathy, were admitted to the Department of Nephrology to establish permanent vascular access for hemodialysis: preferably AVF. Temporary dialysis catheters were implanted in uremic emergency to bridge the time to fistula creation/maturation. AVF was attempted in 87.2% of the patients. Primary AVF function was achieved in 54% of the patients. Cumulative proportional survival of AVF at months 12 and 24 was 81.5%. Ninety‐four percent of AVF were localized on the forearm: 74% in the distal and 20% in the proximal part. Mean duration of hemodialysis therapy was 20.80 ± 19.45 months. The mean time of AVF use was 15.9 ± 20.2 months. Until present, 38% have been dialyzed using AVF for 31.0 ± 18.8 months. Five patients died with functioning fistula. Eight patients initiated hemodialysis therapy with fistula. During further observation, the use of AVF increased to 62%. Elderly patients should not be denied creation of AVF as a rule. The outcome of AVF benefits more from acknowledging individual vascular conditions rather than age of the patient. 相似文献
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