HSV-tk基因/GCV系统治疗肿瘤的旁观者效应与缝隙连接关系的研究

目的 探讨单纯疱疹病毒胸苷激酶（HSV－tk）基因／丙氧鸟苷（GCV）系统杀伤肿瘤细胞时旁观者效应与细胞间缝隙连接的关系。方法 用PA317细胞包装STK质粒（逆转录病毒载体介导的HSV-tk基因），形成假逆转录病毒颗粒，转染有缝隙连接的大鼠胶质瘤细胞C6和无缝隙连接的入宫颈癌细胞 Hela，制成 C6 tk+和 Helatk+细胞，将不同比例的C6和 C6 tk+、Hela和 Hela tk+细胞混合，每种比例8孔，培养 24h后 4孔加入含0．5μg/mlGCV的培养基，4孔作对照，6d后用MTT法测量细胞存活率。在两个培养皿中放入周围粘有33mm高滤纸的载玻片，分别接种 C6、C6 tk+细胞。细胞 80%汇合后将载玻片互换，并加入含0．5μg/ml GCV的培养基，6d后观察细胞形态。结果C6细胞组存在旁观者效应，Heal细胞组不存在旁观者效应。培养皿中tk+细胞大部分被杀死,tk-细胞无变化。结论HSV-tk基因/GCV系统杀伤肿瘤细胞时旁观者效应与细胞间缝隙连接有关。     

Anisotropic properties of aligned SWNT modified poly (methyl methacrylate) nanocomposites

The poly (methyl methacrylate) (PMMA)/single-walled carbon nanotube (SWNT) composites with good uniformity, dispersion and alignment of SWNT were fabricated in an improved figuration process. The semidried mixture was stretched along one direction at a drawing ratio of 50 before it was dried, and then folded along the same direction stretching repeatedly for 100 times. The transmission electron microscopic (TEM) observation demonstrated that SWNT in the PMMA/SWNT composite tends to align in the stretching direction owing to a torque exerting on it in the stretching process. The electrical and mechanical properties of PMMA/SWNT composite were studied as a function of SWNT orientation and concentration. The aligned SWNT modified PMMA/SWNT composite presented highly anisotropic properties. The experimental results showed that the electrical conductivity and mechanical properties of composite rise with the increase of SWNT concentration, and that composite films showed higher conductivity and higher mechanical draw ratios along the stretched direction than perpendicular to it. The thermogravimetric analysis (TGA) revealed that embedding the SWNTs into the PMMA matrix also improves the thermal stability of the composite.     

无线网络安全的风险评估

本文讨论了对无线网络安全的风险评估方法,首先对影响无线网络安全的风险因素进行识别,然后采用AHP法和模糊逻辑法相结合的方法对无线网络系统进行风险评估。通过计算系统的风险度,衡量整个无线网络系统的风险等级。     

基于中间件的Web服务和网格服务混合合成

随着Web服务和网格服务数量急剧增加,通过agent协作的两种服务混合合成却无法解决从安全性到事务处理等问题。本文利用中间件技术,对Web服务和网格服务混合合成框架进行了扩展;并利用面向方面的方法灵活了服务的合成。最终解决了Web服务和网格服务混合合成中安全性、可靠性、一致性、动态性等问题。     

网络综合监控技术

网络综合监控系统是将入侵检测技术,旁路式网络审计相结合的一套综合系统,在不影响网络性能的前提下实现对网络主机的全面监测。提供多种报警手段,对违规事件进行实时报警和阻断,有效防止内部信息泄露与被窃行为。     

Strain rate sensitivity of tensile properties in high Nb containing TiAl alloys

The effect of strain rate on the yield strength of high Nb containing TiAl alloy was studied. The results show that the strain rate sensitivity varies with the test temperature, and the yield strength is not sensitive to the strain rate at room temperature but significantly sensitive to the strain rate at high temperature. An increase of the strain rate or a decrease of the temperature results in an obvious change of fracture mode. It is found that the strain rate sensitivity of this alloy varying with temperature is due to the dislocation climb generated at high temperature.     

马氏体相变的界面吸引子模型

从经典的马氏体相界面运动方程出发,利用相平面分析,证明具有界面摩擦的马氏体相界面是吸引子,与理想的马氏体相界面的孤立子特征不同.通过对非线性方程解的分析,得到发生应力诱发马氏体相变的相界面趋向为焦点型定常吸引子,而热诱发相变的马氏体相界面趋向为结点型定常吸引子,因此考虑界面摩擦后相界面的运动与理想相界面的运动具有不同的非线性特征.     

Rocking stiffness of mounting arrangements in electrical cabinets and control panels

Several studies have shown that the consideration of a rigid body-rocking mode in a cabinet is necessary to evaluate accurate incabinet spectra. Observations from finite element analyses are used to study cabinet rocking behavior and to show that accurate representation of the boundary conditions at the cabinet base is essential in the evaluation of correct rocking mode. Simple formulations for evaluating the rocking stiffness are developed by conducting detailed analytical studies for three different types of cabinet mounting arrangements. Availability of these formulations enables incorporation of a cabinet rocking mode in the Ritz vector approach [Nucl. Eng. Des. 190 (1990) 225] for evaluating the cabinet dynamic properties in significant mode and for generating the incabinet response spectra.     

一种无组合器的面向群体密码系统的改进方案

通过修改消息加密方式，该文提出了一种面向群体的密码系统改进方案。新的方案不仅避免了同谋攻击，而且简化了原方案的执行步骤，降低了通信量。新方案具有更理想的性能．并且容易实现。     

电网继电保护及故障信息处理系统关键技术的探讨

根据湖南电网继电保护及故障录波信息处理系统的开发与应用实践 ,以及对国内现有模式和应用情况的分析 ,对该系统中子站设计、远程数据传输、信息组织和高层应用等关键技术问题进行了探讨 ,并给出了部分试验数据和试验结论