Fe<Subscript>3</Subscript>O<Subscript>4</Subscript>@PAM@NTA-Ni<Superscript>2+</Superscript> Magnetic Composite Nanoparticles for Highly Specific Separation of His-tagged Proteins

A facile approach has been developed to synthesize Fe₃O₄@PAM (polyacrylamide) nanoparticles (NPs) with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe₃O₄ NPs aqueous suspension. Nitrilotriacetic acid (NTA) was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni²⁺ ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins (His-tagged GFP). The high magnetic property of Fe₃O₄@PAM@NTA-Ni²⁺ allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity (42 μg protein/mg of NPs). The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale.     

CCD传感器实时检测系统在微细电火花加工机床上校正法的研究

CCD(Charge-Couple Device)传感器实时检测系统是一套基于光电检测技术的质量监测仪器,可用于实时观测和测量被加工工件,特别适合安装应用于微细电火花加工机床.本文就该系统安装在电火花加工机床上后如何提高检测精度进行研究,提出了3种方法对该检测系统进行校正,如,整体校准常数、C值校正、D值校正.此后设计实验来检验校准后该系统的稳定性和精确度,从而验证了所提出的三步校正法的可靠性.     

智能卡话者确认系统的研究

介绍了一个将参考模板存放在智能卡上的与相关的话者确认系统及其工作原理、相关算法和参数设置。     

变频调速器在供热锅炉中的应用

本文阐述了变频调速器的节能原理.以哈密基地1锅炉房工程为例,介绍了其在锅炉自动控制系统中的应用,并对其经济效益和社会效益进行了分析.     

厚壁容器圆柱壳开孔应力集中问题(Ⅱ)

根据厚壁圆筒形压力容器开孔应力集中系数的一般表达式，利用正交函数展开的方法，将厚壁圆柱壳的开孔应力问题简化为求解一组无穷代数方程组问题为说明复变函数分析方法的适用性，给出了圆筒形压力容器在自由边界条件下，开圆孔和椭圆孔应力集中系数的数值结果     

湿法冶炼浸出过程H∞优化控制研究与设计

应用H∞优化控制方法对锌湿法冶炼浸出过程进行H∞优化控制器设计,同时,讨论了如何把浸出过程控制问题转化为H∞优化控制问题、浸出过程的输入输出量和H∞标准控制问题各量之间的关系及其物理意义.通过仿真实验,给出了几种情况下的仿真曲线,并进行了分析,从而得出结论.     

Effect of H_2 dilution on the structure and properties of nc-CrC/a-C:H coatings deposited by a hybrid beams system

Nanocomposite Cr C/hydrogenated amorphous carbon(nc-CrC/a-C:H) coatings were deposited by a hybrid beams system comprised of a hollow cathode ion source and a cathodic arc ion-plating unit with varying H_2 flow rates. The influences of H_2 flow rates on the morphologies, microstructures, and properties of the coatings were systematically studied. The morphologies and microstructures of the coatings were characterized by SEM, AFM, XPS, Raman spectroscopy, GIXRD, and HRTEM. The mechanical and tribological properties were measured by a nano-indenter, scratch tester, and ball-ondisk tribometer. The wear tracks were evaluated using 3D profilometer, optical microscope, and EDS analysis. It has been found that a moderate H_2 flow rate can effectively smooth the surface, enlarge the fraction of a sp~3 bond, and improve the properties. The coating exhibits the highest hardness and elastic modulus at the H_2 flow rate of 40 sccm. A superior combination of adhesion strength,friction coefficient, and wear resistance can be achieved at the H_2 flow rate of 80 sccm.     

金属拱型屋面板的开发与应用

简要介绍了武钢Ｗ６６６金属拱型层面板的开发、设计、试验研究及应用情况,探讨了理论分析的方法和应用前景。     

Invariant Deformation Element Model Interpretation to the Crystallography of Diffusional Body-Centered-Cube to Face-Centered-Cube Phase Transformations

The crystallography of body-centered-cube to face-centered cube (bcc-to-fcc) diffusion phase transformations in a duplex stainless steel and a Cu-Zn alloy, including long axis, orientation relationship (OR), habit plane (HP), and dislocation spacing, is successfully interpreted with one-step rotation from the Bain lattice relationship by applying a simplified invariant line (IL) analysis. It is proposed that the dislocation slipping direction in the matrix plays an important role in controlling the crystallography of precipitation.     

城市“社区化”产住一体的新回归模式

中国城市增长的重要形态是郊区型开发区扩张,这种扩张,因功能单一而容易导致城市居民就业与居住的高度分离,并引发了一系列城市社会问题。反之,社区化形态的都市产业空间与生活开发,有助于实现产住一体化,以及产业、居住、商业、生活功能的聚合,从而更好地实现城市集约化、便利化和精明化增长。