The physiochemical composition of durum wheat cultivars was studied in order to investigate the influence of vitreousness on the chemical composition of starch and its thermal and pasting properties. Six durum wheat lines were chosen and grown in northern Syria. Grains of each cultivar were visually sorted according to the degree of vitreousness into fully vitreous and fully starchy fractions. Amylose/amylopectin ratio and total starch was determined using Megazyme methods, while thermal and pasting properties were determined using DSC and RVA. Starchy kernels were higher in total starch than vitreous kernels but showed a decreased amylose content. Negative linear relations were found between amylose content, and both peak viscosity and breakdown. Trends in variation of gelatinisation characteristics were observed between vitreous and starchy kernels from the same cultivar, with higher total enthalpy being associated with starchy grains compared with vitreous grains of the same line. 相似文献
Excessive stimulation of the N-methyl-d-aspartate (NMDA)-type glutamate receptor has been implicated in the neuronal death resulting from focal hypoxia-ischemia. Certain neurosteroids, steroids synthesized de novo in the central nervous system (CNS), have been shown to modulate the action of neurotransmitters at their cellular receptors. Pregnenolone sulfate (PS) is an abundant neurosteroid that enhances the current evoked by NMDA. Using the Ca2+-sensitive fluorescent dye, Fluo-3, AM, and a trypan blue exclusion assay, we evaluated the ability of PS to modulate NMDA-induced changes in intracellular free calcium concentration ([Ca2+]i) and neuronal death in primary cultures of rat hippocampal neurons. The results demonstrate that PS potentiates NMDA-induced increases in [Ca2+]i by 150%. Further, PS exacerbates the MK-801-sensitive neuronal death produced by acute (PS EC50=37 microM) or chronic NMDA exposure, reducing the EC50 of NMDA from 13 to 4 microM under chronic exposure conditions, whereas pregnenolone is ineffective. Our results show that PS, or related sulfated neurosteroids, may play a role in the onset of excitotoxic neuronal death in vivo. 相似文献
BACKGROUND: Apoptosis that has been reported in human atherosclerosis may contribute to the remodeling of atherosclerotic plaques. The identification of specific markers for apoptosis in these plaques would permit the development of specific therapeutic strategies to limit their progression. Cysteine protease CPP-32 is essential for apoptotic death in mammalian cells and appears to be an attractive candidate. METHODS AND RESULTS: We studied 12 atherosclerotic plaques from 12 patients who underwent carotid endarterectomy. Apoptosis was analyzed by in situ end labeling of fragmented DNA (TUNEL method) and corroborated by the presence of DNA fragmentation in agarose gel electrophoresis. CPP-32 was detected with the use of a specific monoclonal antibody, and its expression was compared with that of interleukin-1beta-converting enzyme (ICE). We showed that CPP-32 was highly expressed in 10 of 12 atherosclerotic plaques and that it colocalized with apoptotic cells. Expression of ICE generally paralleled that of CPP-32, but ICE was also detected in plaques negative for CPP-32 and showing no apoptosis. CONCLUSIONS: CPP-32 is highly expressed within human atherosclerotic plaques and is closely related to apoptosis. This finding suggests that CPP-32 may be the ICE-like enzyme responsible for apoptosis in human atherosclerosis and opens new perspectives for the development of therapeutic strategies to alter the progression of this disease. 相似文献
The enantioselective hydrogenation of ethyl pyruvate to (R)- or (S)-ethyl lactate has been studied over alumina- and carbon-supported Pt-metal catalysts modified by various heterocyclic N-compounds and substituted amides. The reactions were carried out under mild conditions in acetic acid; other solvents had a detrimental effect on enantioselectivity. An enantiomeric excess (ee) of 67% and a rate acceleration by a factor of 6, compared to the unmodified catalyst, was observed with alumina-supported Pt modified by (R)-1-(1-naphthyl)ethylamine. In contrast, carbon-supported Pd, Ru and Rh were non-selective and only little active. The studies indicated that besides naphthyl or quinolyl groups, two separate phenyl groups or one phenyl group together with two amino groups can provide a suitable anchoring of the chiral modifier on the Pt surface. The nature of interaction between the modifiers and ethyl pyruvate is briefly discussed. 相似文献
Hydrogenation of 4-methoxy-6-methyl-2-pyrone 1 has been investigated over cinchona-modified Pd/TiO2. The appropriate start-up procedure including a catalyst reduction—oxidation—reduction cycle and short pretreatment with the modifier in the absence of reactant can remarkably enhance the enantiomeric excess (ee) to the dihydropyrone 2. Another key parameter is the alkaloid/Pds molar ratio; the alkaloid concentration in the slurry or the alkaloid/reactant ratio is not crucial. Under the best conditions 94% ee and 95% chemoselectivity to 2 were achieved at 80% conversion of 1, in only 30 min reaction time under ambient conditions. The ee can be further increased by kinetic resolution of 2. In the second reaction step the diasteroselectivity to the cis-tetrahydropyrone 3 is about 99%. 相似文献
A planar single-ended GaAs Schottky diode mixer has been designed, built, and tested at 119 GHz. The mixer front end includes also a waveguide filter for image rejection, and a temperature compensated ring filter. Measurements at room temperature showed a conversion loss of 7 dB and a noise temperature of 900 K (SSB). At 100 K the measured noise temperature of the mixer was 500 K (SSB). 相似文献
The present study reveals a cost benefit process in an open system for utilizing desert sand for preparing sodium silicate or precipitated silica. A simple alkali fusion method has been developed to prepare sodium silicate directly from sand and finally pure precipitated silica by acid precipitation. The reaction weight ratio of alkali to sand reaction parameters are studied for optimizing the silica yield. About 80% pure precipitated silica has been prepared in an open system at 150 °C within 45 min. Wet chemical methods, FTIR, TG-DTA, XRD and SEM techniques are used to characterize the silica prepared from sand available in Saudi Arabian deserts. In the XRD pattern, the peak corresponding to silica was obtained at the diffraction angle of 21.8 ° and it was found to be amorphous in nature. 相似文献
One common concern with video games today is the lack of physical activity they demand from the user. The design of games and tangible user interfaces (TUIs) that stimulate players and engage them into fun exercising activities is starting to attract the attention of many researchers and companies. This paper presents the software and hardware design and development of a TUI intended for exercise-based games targeted mostly towards children. The proposed TUI, SmartPads, can be constructed using elemental building blocks (pads) into numerous shapes. The pads-which are individually controlled by microcontrollers-are mapped onto a computer screen in real-time. A user interacts with the TUI by stepping on the pads. To evaluate the functionality and efficiency of the TUI, we developed three games in the field of exergaming. The games also have an educational value and are integrated with multimedia output modalities to enrich children’s playing experience.
The binary version of the school timetabling (STT) problem is a real‐world example of a constraint network that includes only constraints of inequality. A new and useful representation for this real‐world problem, the STT_Grid, leads to a generic decomposition technique. The paper presents proofs of necessary and sufficient conditions for the existence of a solution to decomposed STT_Grids. The decomposition procedure is of low enough complexity to be practical for large problems, such as a real‐world high school. To test the decomposition approach, a typical high school was analyzed and used as a model for generating STT_Grids of various sizes. Experiments were conducted to test the difficulty of large STT networks and their solution by decomposition. The experimental results show that the decomposition procedure enables the solution of large STT_Grids (620 variables for a real school) in reasonable time. The constraint network of a typical STT_Grid is sparse and belongs to the class of easy problems. Still, due to the sizes of STTs, good constraint satisfaction problem search techniques (i.e., BackJumping and ForwardChecking) do not terminate in reasonable times for STT_Grids that are larger than 300 variables. 相似文献