Leak detection in pipe using transient flow and genetic algorithm

An inverse transient method with genetic algorithm (GA) was applied to leak detection in pipeline. Transient flow caused by valve operation was calculated using the characteristics method. The location and discharge of leak were determined so that the difference of the calculated pressure may be minimized from the reference pressure calculated under a given leak condition. Calculations were done for the leak at one and two locations in pipeline. Furthermore, the effect of noise in pressure data was discussed, and the leak locations and leak discharges can be predicted precisely even in the case of noisy data. This paper was presented at the 9^th Asian International Conference on Fluid Machinery (AICFM9), Jeju Korea, October 16–19, 2007.     

Kinematics, kinetics and muscle activities of the lower extremity during the first four steps from gait initiation to the steady-state walking

In this study, biomechanical characteristics during the whole process of gait initiation for twenty normal healthy volunteers were determined by the motion analysis with six near-infrared cameras, four forceplates, and an EMG system. Gait initiation, a transitional movement phenomenon from quiet stance to steady-state walking, involves a series of muscular activities, GRFs, movements of COP and COM, and joint motions. Results showed that the location of the net COP to be most lateral during double limb stance at the beginning of gait initiation. During gait initiation, changes in anteroposterior components of GRFs were first found and then changes in vertical components followed. Hip and knee motions were found before the ankle joint motion. Walking speed, step length, and stride length gradually increased until the second step. The interaction between the COM and COP is tightly regulated to control the trajectory of the COM and thereby control total body balance. This paper was recommended for publication in revised form by Associate Editor Hong Hee Yoo Sun-Woo Park received a B.S. degree in Biomedical Engineering from Yonsei University in 2006. He is currently a M.S. candidate in the Department of Biomedical Engineering, Yonsei University, Korea. His research interests in the area of Human Movement and detection of gait phase using motion sensors. Hue-Seok Choi received a B.S. degree in Computer Engineering from Daejeon University in 2004. He is currently a P.D. candidate at the Department of Biomedical Engineering at Yonsei University, Korea. His research interests are in the area of Human Movement and Rehabilitation Engineering. Ki-Hong Ryu received a B.S. and M.S. degrees in Biomedical Engineering from Yonsei University in 1998 and 2001, respectively. He is currently a P.D. candidate in the Department of Biomedical Engineering, Yonsei University, Korea. His research interests are in the area of Human Movement and Gait Training System using Functional Electrical Stimulation. Sa-Yup Kim received a B.S. degree in Electrical Engineering from Yeungnam University in 2002 and M.S. degrees in Biomedical Engineering from Yonsei University in 2006. He is currently working from Korea Institute of Industrial Technology in Cheonan, Korea. Young-Ho Kim received a B.S. degree in Mechanical Engineering from Hanyang University in 1982. He then went on to receive his M.S. and Ph.D. degrees from University of Iowa in 1989 and 1991, respectively. He is currently a Professor in the Department of Biomedical Engineering, Yonsei University, Korea. His research interests are in the area of Human Movement, Rehabilitation Engineering, and Biomechanics.     

Endometrial Epithelial ARID1A Is Required for Uterine Immune Homeostasis during Early Pregnancy

A growing body of work suggests epigenetic dysregulation contributes to endometriosis pathophysiology and female infertility. The chromatin remodeling complex subunit AT-rich interaction domain 1A (ARID1A) must be properly expressed to maintain normal uterine function. Endometrial epithelial ARID1A is indispensable for pregnancy establishment in mice through regulation of endometrial gland function; however, ARID1A expression is decreased in infertile women with endometriosis. We hypothesized that ARID1A performs critical operations in the endometrial epithelium necessary for fertility besides maintaining gland function. To identify alterations in uterine gene expression resulting from loss of epithelial ARID1A, we performed RNA-sequencing analysis on pre-implantation uteri from Ltf^iCre/+Arid1a^f/f and control mice. Differential expression analysis identified 4181 differentially expressed genes enriched for immune-related ingenuity canonical pathways including agranulocyte adhesion and diapedesis and natural killer cell signaling. RT-qPCR confirmed an increase in pro-inflammatory cytokine and macrophage-related gene expression but a decrease in natural killer cell signaling. Immunostaining confirmed a uterus-specific increase in macrophage infiltration. Flow cytometry delineated an increase in inflammatory macrophages and a decrease in uterine dendritic cells in Ltf^iCre/+Arid1a^f/f uteri. These findings demonstrate a role for endometrial epithelial ARID1A in suppressing inflammation and maintaining uterine immune homeostasis, which are required for successful pregnancy and gynecological health.     

A Flower-like In2O3 Catalyst Derived via Metal–Organic Frameworks for Photocatalytic Applications

The most pressing concerns in environmental remediation are the design and development of catalysts with benign, low-cost, and efficient photocatalytic activity. The present study effectively generated a flower-like indium oxide (In₂O₃-MF) catalyst employing a convenient MOF-based solvothermal self-assembly technique. The In₂O₃-MF photocatalyst exhibits a flower-like structure, according to morphology and structural analysis. The enhanced photocatalytic activity of the In₂O₃-MF catalyst for 4-nitrophenol (4-NP) and methylene blue (MB) is likely due to its unique 3D structure, which includes a large surface area (486.95 m² g⁻¹), a wide spectrum response, and the prevention of electron–hole recombination compared to In₂O₃-MR (indium oxide-micro rod) and In₂O₃-MD (indium oxide-micro disc). In the presence of NaBH₄ and visible light, the catalytic performances of the In₂O₃-MF, In₂O₃-MR, and In₂O₃-MD catalysts for the reduction of 4-NP and MB degradation were investigated. Using In₂O₃-MF as a catalyst, we were able to achieve a 99.32 percent reduction of 4-NP in 20 min and 99.2 percent degradation of MB in 3 min. Interestingly, the conversion rates of catalytic 4-NP and MB were still larger than 95 and 96 percent after five consecutive cycles of catalytic tests, suggesting that the In₂O₃-MF catalyst has outstanding catalytic performance and a high reutilization rate.     

Bioinformatics of Differentially Expressed Genes in Phorbol 12-Myristate 13-Acetate-Induced Megakaryocytic Differentiation of K562 Cells by Microarray Analysis

Megakaryocytes are large hematopoietic cells present in the bone marrow cavity, comprising less than 0.1% of all bone marrow cells. Despite their small number, megakaryocytes play important roles in blood coagulation, inflammatory responses, and platelet production. However, little is known about changes in gene expression during megakaryocyte maturation. Here we identified the genes whose expression was changed during K562 leukemia cell differentiation into megakaryocytes using an Affymetrix GeneChip microarray to determine the multifunctionality of megakaryocytes. K562 cells were differentiated into mature megakaryocytes by treatment for 7 days with phorbol 12-myristate 13-acetate, and a microarray was performed using RNA obtained from both types of cells. The expression of 44,629 genes was compared between K562 cells and mature megakaryocytes, and 954 differentially expressed genes (DEGs) were selected based on a p-value < 0.05 and a fold change >2. The DEGs was further functionally classified using five major megakaryocyte function-associated clusters—inflammatory response, angiogenesis, cell migration, extracellular matrix, and secretion. Furthermore, interaction analysis based on the STRING database was used to generate interactions between the proteins translated from the DEGs. This study provides information on the bioinformatics of the DEGs in mature megakaryocytes after K562 cell differentiation.     

Direct vapor phase growth process and robust photoluminescence properties of large area MoS2 layers

There has been growing research interest in the use of molybdenum disulfide in the fields of optoelectronics and energy harvesting devices, by virtue of its indirect-to-direct band gap tunability. However, obtaining large area thin films of MoS2 for future device applications still remains a challenge. In the present study, the amounts of the precursors （S and MOO3） were varied systematically in order to optimize the growth of highly crystalline and large area MoS2 layers by the chemical vapor deposition method. Careful control of the amounts of precursors was found to the key factor in the synthesis of large area highly crystalline flakes. The thickness of the layers was confirmed by Raman spectroscopy and atomic force microscopy. The optical properties and chemical composition were studied by photoluminescence （PL） and X-ray photoelectron spectroscopy. The emergence of strong direct excitonic emissions at 1.82 eV （A-exciton, with a normalized PL intensity of -55 × 10^3） and 1.98 eV （B-exciton, with a normalized PL intensity of -5 × 10^3） of the sample at room temperature clearly indicates the high luminescence quantum efficiency. The mobility of the films was found to be 0.09 cm^2/（V.s） at room temperature. This study provides a method for the controlled synthesis of high-quality two-dimensional （2D） transition metal dichalcogenide materials, useful for applications in nanodevices, optoelectronics and solar energv conversion.     

Effect of surface structure on biomechanical properties and osseointegration

The purpose of this study is to improve the bone-bonding ability between titanium implants and living bone through the control of geometric design and chemical compositions of an implant surface. We compared the tissue healing response and resulting implant stability for three surface designs by characterizing the histological and mechanical properties of the healing tissue around smooth-surfaced Ti–6Al–4V (SS), CP-Ti plasma-spray-coated (PSC), alkali- and heat-treated (AHT) implants. The implants were transversely inserted into a dog thighbone and evaluated at 4, 8, and 12 weeks. Histological examination indicated that initial matrix mineralization leading to osseointegration occurred more rapidly with the AHT implant. During the 4, 8, and 12 week healing periods, new bone on the surface of AHT implant showed denser growth than that on the SS and PSC implants. The more extensive tissue integration and more rapid matrix mineralization with the AHT implant were reflected in the mechanical test data, which demonstrated superior attachment strength and interfacial stiffness for the AHT implant after healing for 4, 8 and 12 weeks of healing because of the mechanical interlocking in the micrometer sized rough surface and the large bonding area between bone and implant caused by the nanosized porous surface structure. Histological and mechanical data demonstrate that with the appropriate surface design selection, bone bone-bonding ability can be improved and can induce acceleration of the healing response, thereby improving the potential for implant osseointegration.     

Enhanced surface-excitonic emission in ZnO/Al(2)O(3) core-shell nanowires

We report the influence of an Al(2)O(3) shell on the photoluminescence emission of ZnO nanowires. At room temperature, the spectrum of the core-shell nanowires shows a strong reduction of the relative intensity of the green defect emission with respect to the near-band-edge emission. At 5?K an increase of the relative intensity of the surface exciton band with respect to the donor-bound exciton emission is observed. Annealing the core-shell nanowires at 500?°C does not increase the green defect luminescence at 5?K. We propose a model explaining the spectral changes.     

Heat-Killed Enterococcus faecalis EF-2001 Induces Human Dermal Papilla Cell Proliferation and Hair Regrowth in C57BL/6 Mice

Minoxidil is the most widely used treatment for hair growth, but has been associated with several side effects. In this study, we investigated the effects of heat-killed Enterococcus faecalis EF-2001 on hair loss prevention and regrowth using human dermal papilla cells and male C57BL/6 mice. To examine the effects of EF-2001, we used minoxidil as the positive control. In the in vitro experiments, EF-2001 treatment (75–500 μg/mL) led to the proliferation of human dermal papilla cells in a concentration-dependent manner. In the in vivo experiment, the topical application of 200 µL EF-2001 on the dorsal surface of C57BL/6 male mice led to hair growth. Changes in hair regrowth were examined by visual comparison and hematoxylin and eosin staining of skin sections. We also determined the expression levels of marker genes (Wnt) and growth factors (fibroblast growth factor, insulin growth factor 1, and vascular endothelial growth factor) in the skin tissues of the back of each mouse using a quantitative polymerase chain reaction. EF-2001 accelerated the progression of hair regrowth in mice and promoted hair-follicle conversion from telogen to anagen, likely by increasing the expression levels of growth factors and marker genes.