Cation/H+ antiporters mediate potassium and sodium fluxes in Pichia sorbitophila. Cloning of the PsNHA1 and PsNHA2 genes and expression in Saccharomyces cerevisiae

Pichia sorbitophila grows rapidly in the presence of very high NaCl concentrations. Under these conditions, even when the K(+) concentration is low, P. sorbitophila cells can maintain low Na(+) and high K(+) contents. This remarkable capacity of P. sorbitophila fails when the external pH is not acidic. This indicates that Na(+) efflux is mediated by an electroneutral Na(+)/H(+) antiporter. We have cloned and sequenced two genes designated as PsNHA1 and PsNHA2, which probably encode two antiporters of this type. The genes present high similarity with the corresponding genes from other yeasts. The heterologous expression of PsNHA1 or PsNHA2 in a Saccharomyces cerevisiae mutant lacking the Na(+) efflux systems and sensitive to high concentrations of Na(+) and K(+) rescued the tolerance and the ability to extrude both cations. The Accession Nos of the sequenced DNA fragments are: PsNHA1, AJ496431; PsNHA2, AJ496432. (TC 2.A.36) Copyright 2002 John Wiley & Sons, Ltd.     

Evanescent wave coupling in light scattering of an off-axis normally incident Gaussian beam by two parallel nonabsorbing cylinders

A theoretical and numerical investigation is devised for resonant light scattering of an off-axis normally incident Gaussian beam by two parallel nonabsorbing cylinders based on the related beam theory developed in J. Opt. Soc. Am. A 14, 640 (1997). By varying the half-beam width, we show that the multireflection process between the two scatterers can be minimized. Moreover, the study is an attempt to understand the underlying physics present in the process of resonance excitation by evanescent wave coupling.     

The Sonogel-Carbon electrode as a sol-gel graphite-based electrode

In this work, a novel sol-gel-based procedure to obtain solid carbon composite electrodes is proposed. The procedure is based on the use of sonocatalysis to apply high-energy ultrasound directly to the precursors; this way, ultrasonic cavitation is achieved so that hydrolysis with acidic water is promoted in the absence of any additional solvent and the time needed to get a unique phase is reduced drastically. The mix of sonogel with spectroscopic grade graphite leads to a new type of sol-gel electrode: the Sonogel-Carbon electrode. The amount of water, necessary for hydrolysis to occur, has been studied, as well as the sol pH value and the electrochemical behavior of the Sonogel-Carbon electrode in Britton-Robinson buffer. Mechanical renewal of the electrodes surfaces and background signal stability for at least 42 days have been also tested. We have carried out some tests to check the ability of the synthesized material to give electrochemical response when redox species are present in the background electrolyte. Mechanical and electrochemical studies indicate that the Sonogel-Carbon electrode shows good properties for use as an electrochemical sensor.     

Characterization of archaeological beeswax by electron ionization and electrospray ionization mass spectrometry

To better detect and identify beeswax in ancient organic residues from archaeological remains, we developed a new analytical methodology consisting of the analysis of (i) the trimethylsilylated organic extract by GC/MS and (ii) the crude extract by ESI-MS. Selective scanning modes, such as SIM or MRM, permit separate quantification of each chemical family (fatty acids, monoesters, monohydroxyesters, and diesters) and allow an improvement in sensitivity and selectivity, allowing the crude extract to be treated without further purification. GC/MS (SIM) was revealed to be a powerful method for the detection of components, with a detection limit down to a total lipid extract in the range of approximately 50 ng in a complex matix, such as archaeological degraded material, whereas ESI-MS/MS is instead used for the detection of nonvolatile biomarkers. Identification by GC/MS (SIM) and ESI-MS/ MS (MRM) of more than 50 biomarkers of beeswax in an Etruscan cup at the parts-per-million level provides the first evidence for the use of this material by the Etruscans as fuel or as a waterproof coating for ceramics.     

Speckle size of light scattered from slightly rough cylindrical surfaces

This research is an extension of the optical method of quality control presented in a previous paper [Appl. Opt. 39, 5811 (2000)] to the case of slightly rough cylindrical surfaces. Applying the Kirchhoff scalar diffraction theory yields an analytical expression of the autocorrelation function of the intensity scattered from slightly rough cylindrical surfaces. This function, which is related to speckle size and shape, is shown to depend on the surface correlation length, unlike for plane surfaces for which the speckle depends on the illuminated area only. The theoretical expression is compared with that for the speckle produced by the light scattered from a cylindrical bearing and from various high-quality wires, showing that the method allows the correlation lengths of high-quality cylindrical surfaces to be determined.     

Measurement of the induced refractive index in a photothermorefractive glass by a liquid-cell shearing interferometer

A liquid-cell shearing interferometer was developed to measure refractive-index variations (delta n) in transparent materials. The cell was filled with a liquid having a matched refractive index. The achieved resolution was better than 1/1000 of a fringe shift and resulted in a delta n measurement sensitivity down to 10(-7) for 1-mm-thick samples. A refractive-index increment in photothermorefractive glass of up to 5 x 10(-6) was observed after UV exposure at 325 nm. A refractive-index decrement of up to 1 x 10(-3) was observed after thermal development of the exposed sample. It was proved that photothermorefractive glass obeys the reciprocity law; i.e., delta n depends on the UV dosage but does not depend on the irradiance.     

Light-scattering measurements of optical thin-film components at 157 and 193 nm

An instrument for total backscattering and forward-scattering measurements of optical coating components at 157 and 193 nm is described. The system is operated in both vacuum and nitrogen purge gas. An excimer laser as well as a deuterium lamp can be used as a radiation source. Suppression of the background signal level to 1 part in 10(6) permits measurements even of low-scatter samples such as superpolished substrates and antireflection coatings. Results of investigations of antireflective and highly reflective multilayers and CaF2 substrates reveal scattering from surface and interface roughness as well as from the volume of the substrate material. First steps to extend the instrument for angle-resolved scatter, transmittance, and reflectance measurements are described.     

Multiwavelength imaging of defects in ultraviolet optical materials

Laser-induced damage in bare glass substrates and thin films has long been widely acknowledged as a localized phenomenon associated with the presence of micrometer and submicrometer scale defects. The scanning of both optical absorption and scattering allows us to discriminate between absorbing and nonabsorbing defects and can give specific information about the origin of the defects. We investigate the spectral properties of defects in thin films and fused-silica surfaces. Absorbing and scattering defects are studied at different wavelengths in the ultraviolet, visible, and infrared ranges. Absorbing defects are shown to be highly wavelength dependent, whereas we have observed significant correlation between scattering defects.     

Optical properties and quantum efficiency of thin-film alkali halides in the far ultraviolet

The optical constants of thin films of CsI, KI, and KBr and the quantum efficiency (QE) of planar photocathodes made with these alkali halides in the 53.6-174.4-nm spectral range are presented. The optical constants were obtained from measurements of the reflectance as a function of incidence angle. The effect of film heating and exposure to UV irradiation on the optical properties and on the QE of the three alkali halides was investigated. KBr was found to be the most stable material for both heating and UV irradiation. KI appeared to be close to temperature stable, whereas UV exposure affected its optical constants. CsI optical constants changed after 420 K heating and after UV exposure. The changes in the optical constants were related to the QE changes, and a certain correlation between both variations was determined. However, it was also demonstrated that the QE changes cannot be explained solely by the changes in optical constants.     

Retrieval of the ultraviolet aerosol optical depth during a spring campaign in the Bavarian Alps

A measurement campaign was organized in March 1999 in the Bavarian Alps as part of the European project, Characteristics of the UV Radiation Field in the Alps (CUVRA), to analyze the effect of altitude, aerosols, and snow cover on ground-level UV spectral irradiance. We present the results of simultaneous measurements of aerosol optical depth (AOD) made at various sites on two cloudless days in March 1999. The two days exhibited different aerosol conditions. Results derived from spectral measurements of UV irradiance are compared with data from filter radiometer measurements made at discrete wavelengths extending from the UV to the near IR. The different methods generated values for the AOD that were in good agreement. This result confirms that one can use either method to retrieve the AOD with an uncertainty of approximately 0.03-0.05. On 18 March, high turbidity was observed at low altitude (400-nm AOD approximately 0.5 at 700 m above sea level), and the AOD decreased regularly with altitude; on 24 March, the turbidity was much less (0.11 at 700 m above sea level). On both days very low AODs (0.05-0.09) were measured at 3000 m above sea level. The spectral dependence of the AOD is often parameterized by the angstrom relationship; the alpha parameter is generally difficult or impossible to retrieve from spectral measurements because of the relatively narrow wavelength range (320-400 nm), and only one of the spectro-radiometers used during the campaign permits this retrieval. In most cases, during this field campaign, alpha was found by filter sunphotometers to be 1.1-1.5.