A novel small conductance Ca2+-activated K+ channel blocker from Oxyuranus scutellatus taipan venom. Re-evaluation of taicatoxin as a selective Ca2+ channel probe

Taicatoxin, isolated from the venom of the Australian taipan snake Oxyuranus scutellatus, has been previously regarded as a specific blocker of high threshold Ca2+ channels in heart. Here we show that taicatoxin (in contrast to a range of other Ca2+ channel blockers) interacts with apamin-sensitive, small conductance, Ca2+-activated potassium channels on both chromaffin cells and in the brain. Taicatoxin displays high affinity recognition of 125I-apamin acceptor-binding sites, present on rat synaptosomal membranes (Ki = 1.45 +/- 0.22 nM) and also specifically blocks affinity-labeling of a 33-kDa 125I-apamin-binding polypeptide on rat brain membranes. Taicatoxin (50 nM) completely blocks apamin-sensitive after-hyperpolarizing slow tail K+ currents generated in rat chromaffin cells (mean block 97 +/- 3%, n = 12) while only partially reducing total voltage-dependent Ca2+ currents (mean block 12 +/- 4%, n = 6). In view of these findings, the use of taicatoxin as a specific ligand for Ca2+ channels should now be reconsidered.     

How do people really seek information about others?: Information seeking across Internet and traditional communication channels

The present research describes the manner in which individuals use various media in the interpersonal information seeking process. Stephens' (2007) information and communication technology (ICT) succession theory was applied to an interpersonal information seeking context, and hypotheses and research questions about the channels people use to seek information about others of various relationship to the seeker were offered. Two hundred and twenty-five participants responded to a survey about this topic, and they reported a greater likelihood to seek information about less-known targets using channels where they would be unidentifiable. However, participants reported a greater likelihood to seek information about more-known targets using channels where they would be identifiable. Channels such as social networking websites were frequently reported to be useful regardless of whether the target was well known or less known. Properties of these channels and their implications for interpersonal information seeking as well as theoretical implications of these findings are discussed and directions for future research are examined.     

Standard delay eyeblink classical conditioning is independent of awareness.

P. F. Lovibond and D. R. Shanks (see record 2002-00340-001) suggested that all forms of classical conditioning depend on awareness of the stimulus contingencies. This article considers the available data for eyeblink classical conditioning, including data from 2 studies (R. E. Clark, J. R. Manns, & L. R. Squire, 2001; J. R. Manns, R. E. Clark, & L. R. Squire, 2001) that were completed too recently to have been considered in their review. In addition, in response to questions raised by P. F. Lovibond and D. R. Shanks, 2 new analyses of data are presented from studies published previously. The available data from humans and experimental animals provide strong evidence that delay eyeblink classical conditioning (but not trace eyeblink classical conditioning) can be acquired and retained independently of the forebrain and independently of awareness. This conclusion applies to standard conditioning paradigms; for example, to single-cue delay conditioning when a tone is used as the conditioned stimulus (CS) and to differential delay conditioning when the positive and negative conditioned stimuli (CS+ and CS-) are a tone and white noise. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

An expert system for the determination and computation of hypothesis tests

We describe the design and development of Hypothesis Tester (HT), an Expert System which not only guides the user to select the proper statistical test, but is also capable of computing the value of the test statistic. HT is designed to test hypotheses of proportions and means, in one and two population samples, under various conditions, such as large or small sample size, Normal or not Normal distribution, known or unknown variance, and equal or unequal variances.     

Formulation of Controlled Release Matrices by Granulation with a Polymer Dispersion

The objective of this work was to incorporate an ethylcellulose-based controlled-release coating suspension (Surelease, Colorcon) within a tablet matrix to provide a release controlling mechanism. Anhydrous theophylline, chlorpheniramine maleate, and acetaminophen were selected as model drug entities. Surelease dispersion was incorporated as the granulating agent either to the drug entity alone or to a blended mixture of drug and filler. Control batches included simple aqueous granulations and direct compression mixtures. Tablets were prepared on a single stroke tablet press. Dissolution was performed by the USP Method I (rotating basket) in purified water for the granulations and the resulting tablets. The uncompressed granulations did not exhibit prolonged release. In general, tablets prepared with the polymer suspension as the granulating agent were non-disintegrating, and exhibited slower dissolution than the control tablets. Release profiles were affected by drug concentration and excipient levels. By the dissolution method selected, complete drug release for the various formulations ranged from less than 1 hour to greater than 12 hours. The use of the polymer dispersion appears to enhance the processing characteristics of some materials, and to provide the formulator with control over drug release.     

Benzocyclobutene (BCB) dielectrics for the fabrication of high density,thin film multichip modules

A new class of organic dielectrics, benzocyclobutenes, 1, are described and their application to the fabrication of thin film multichip modules is detailed. Key properties for3, a siloxy containing BCB derivative include low dielectric constant (2.7), low loss (0.008 at 1 MHz), low water absorption (0.25% after 24 h water boil) and high degree of planarization (>90% from one layer coverage). All other properties meet the requirements necessary for fabrication of thin film MCM structures.     

Modelling knowledge and action in distributed systems

We present a formal model that captures the subtle interaction between knowledge and action in distributed systems. We view a distributed system as a set ofruns, where a run is a function from time toglobal states and a global state is a tuple consisting of anenvironment state and alocal state for earch process in the system. This model is a generalization of those used in many previous papers.Actions in this model are associated with functions from global states to global states. Aprotocol is a function from local states to actions. We extend the standard notion of a protocol by definingknowledge-based protocols, ones in which a process' actions may depend explicitly on its knowledge. Knowledge-based protocols provide a natural way of describing how actions should take place in a distributed system. Finally, we show how the notion of one protocolimplementing another can be captured in our model. Joseph Y. Halpern received a B.Sc. in mathematics from the University of Toronto in 1975, and a Ph.D. in mathematics from Harvard University in 1981. In between, he spent two years as the head of the Mathematics Department at Bawku Secondary School, in Ghana. After a year as a visiting scientist at MIT, he joined IBM in 1982. He is currently the manager of the Mathematics and Related Computer Science Department at the IBM Almaden Research Center, and a consulting professor in the Computer Science Department at Stanford. His major research interests are reasoning about knowledge, distributed computation, and logics of programs. He was program chairman and organizer of the first conference of Theoretical Aspects of Reasoning About Knowledge, program chairman of the Fifth ACM Symposium on Principles of Distributed Computing, and was the co-recipient (with Ronald Fagin) of the MIT Publisher's Prize for the Best Paper Paper at the 1985 International Joint Conference on Artificial Intelligence. Ronald Fagin is manager of the Foundations of Computer Science group at the IBM Almaden Research Center. He received his B.A. degree in mathematics from Dartmouth College in 1967 and his Ph.D. in mathematics, specializing in mathematical logic, from the University of California at Berkeley in 1973. He joined IBM in 1973 at the Thomas J. Watson Research Center. In 1975, he transferred to the San Jose Research Laboratory (now the IBM Almaden Research Center) where most of his research has centered on applications of logic to computer science. In particular, he has done research on the theory of relational databases and, more recently, on theories of knowledge and belief. He has received three IBM Outstanding Innovation Awards for his contributions to relational database theory, extendible hashing, and reasoning about knowledge. He was co-recipient (with Joe Halpern) of the MIT Press Publisher's Prize for the Best Paper at the 1985 International Joint Conference on Artificial Interlligence.Some material in this paper appeared in preliminary form in Halpern and Fagin (1985). An abridged version of the paper appeared in Vogt F (ed) Proceeding of Concurrency 88 (Lecture Notes in Computer Science Vol. 335) Springer-Verlag, 1988, pp 18–32