Single-Cell Microgels for Diagnostics and Therapeutics

Cell encapsulation within hydrogel droplets is transforming what is feasible in multiple fields of biomedical science such as tissue engineering and regenerative medicine, in vitro modeling, and cell-based therapies. Recent advances have allowed researchers to miniaturize material encapsulation complexes down to single-cell scales, where each complex, termed a single-cell microgel, contains only one cell surrounded by a hydrogel matrix while remaining <100 μm in size. With this achievement, studies requiring single-cell resolution are now possible, similar to those done using liquid droplet encapsulation. Of particular note, applications involving long-term in vitro cultures, modular bioinks, high-throughput screenings, and formation of 3D cellular microenvironments can be tuned independently to suit the needs of individual cells and experimental goals. In this progress report, an overview of established materials and techniques used to fabricate single-cell microgels, as well as insight into potential alternatives is provided. This focused review is concluded by discussing applications that have already benefited from single-cell microgel technologies, as well as prospective applications on the cusp of achieving important new capabilities.     

Detailed Balance Broken by Catch Bond Kinetics Enables Mechanical-Adaptation in Active Materials

Unlike nearly all engineered materials which contain bonds that weaken under load, biological materials contain “catch” bonds which are reinforced under load. Consequently, materials, such as the cell cytoskeleton, can adapt their mechanical properties in response to their state of internal, non-equilibrium (active) stress. However, how large-scale material properties vary with the distance from equilibrium is unknown, as are the relative roles of active stress and binding kinetics in establishing this distance. Through course-grained molecular dynamics simulations, the effect of breaking of detailed balance by catch bonds on the accumulation and dissipation of energy within a model of the actomyosin cytoskeleton is explored. It is found that the extent to which detailed balance is broken uniquely determines a large-scale fluid-solid transition with characteristic time-reversal symmetries. The transition depends critically on the strength of the catch bond, suggesting that active stress is necessary but insufficient to mount an adaptive mechanical response.     

Bend,Twist, and Turn: First Bendable and Malleable Toughened PLA Green Composites

The future of green electronics possessing great strength and toughness proves to be a promising area of research in this technologically advanced society. This work develops the first fully bendable and malleable toughened polylactic acid (PLA) green composite by incorporating a multifunctional polyhydroxybutyrate rubber copolymer filler that acts as an effective nucleating agent to accelerate PLA crystallization and performs as a dynamic plasticizer to generate massive polymer chain movement. The resultant biocomposite exhibits a 24‐fold and 15‐fold increment in both elongation and toughness, respectively, while retaining its elastic modulus at >3 GPa. Mechanism studies show the toughening effect is due to an amalgamation of massive shear yielding, crazing, and nanocavitation in the highly dense PLA matrix. Uniquely distinguished from the typical flexible polymer that stretches and recovers, this biocomposite is the first report of PLA that can be “bend, twist, turn, and fold” at room temperature and exhibit excellent mechanical robustness even after a 180° bend, attributes to the highly interconnected polymer network of innumerable nanocavitation complemented with an extensively unified fibrillar bridge. This unique trait certainly opens up a new horizon to future sustainable green electronics development.     

Liquid Metal Based Island‐Bridge Architectures for All Printed Stretchable Electrochemical Devices

The adoption of epidermal electronics into everyday life requires new design and fabrication paradigms, transitioning away from traditional rigid, bulky electronics towards soft devices that adapt with high intimacy to the human body. Here, a new strategy is reported for fabricating achieving highly stretchable “island‐bridge” (IB) electrochemical devices based on thick‐film printing process involving merging the deterministic IB architecture with stress‐enduring composite silver (Ag) inks based on eutectic gallium‐indium particles (EGaInPs) as dynamic electrical anchors within the inside the percolated network. The fabrication of free‐standing soft Ag‐EGaInPs‐based serpentine “bridges” enables the printed microstructures to maintain mechanical and electrical properties under an extreme (≈800%) strain. Coupling these highly stretchable “bridges” with rigid multifunctional “island” electrodes allows the realization of electrochemical devices that can sustain high mechanical deformation while displaying an extremely attractive and stable electrochemical performance. The advantages and practical utility of the new printed Ag‐liquid metal‐based island‐bridge designs are discussed and illustrated using a wearable biofuel cell. Such new scalable and tunable fabrication strategy will allow to incorporate a wide range of materials into a single device towards a wide range of applications in wearable electronics.     

Reversing course: Issues in modeling legacy systems

The Enhanced Traffic Management System (ETMS) is a legacy system used by the Federal Aviation Administration (FAA) to support air traffic flow management. Air traffic flow management is the strategic control of air traffic to minimize delays and congestion and maximize the throughput of aircraft throughout the National Airspace System (NAS). This paper discusses the reasons for modeling a legacy system, problems and advantages encountered in modeling an operational system, and describes the construction of a simulation model of ETMS. Originally written in Pascal to run on Apollo workstations under the Aegis Domain operating system, ETMS has been converted to C/C++ and ported to HP servers and workstations running HP-UX, a POSIX-compliant version of UNIX. The objectives of the modeling task were to assess performance of the ported system and to provide a basis for evaluating a possible redesign/re-architecture of the system. The initial plan was to develop one or two models aimed at the network aspects (both LAN and WAN) of ETMS at a relatively high level, and then to develop a more detailed model to look at specific workstation/server issues.As is shown in this paper, issues of existing system design and documentation and the availability (or the lack) of data continually arose. Nevertheless, a reasonable set of working assumptions were derived which allowed modeling and evaluation to proceed. Thus, the quantitative and qualitative results obtained provided information and lessons learned that can be built upon. Moreover, the second of the stated goals (to provide a basis for possible redesign) was also achieved because there is now a baseline for future design/architecture studies. The focus of this paper is to provide insights into the issues involved in modeling an existing system rather than the results of the model itself.     

An investigation of surface state capture cross-sections for metal–oxide–semiconductor field-effect transistors using HfO2 gate dielectrics

MOSFETs and MOSCs incorporating HfO₂ gate dielectrics were fabricated. The I_DS–V_DS, I_DS–V_GS, gated-diode and C–V characteristics were investigated. The subthreshold swing and the interface trap density were obtained. The surface recombination velocity and the minority carrier lifetime in the field-induced depletion region measured from the gated diodes were about 2.73 × 10³ cm/s and 1.63 × 10⁻⁶ s, respectively. The effective capture cross section of surface state was determined to be 1.6 × 10⁻¹⁵ cm² using the gated-diode technique in comparison with the subthreshold swing measurement. A comparison with conventional MOSFETs using SiO₂ gate oxide was also made.     

On‐Body Bioelectronics: Wearable Biofuel Cells for Bioenergy Harvesting and Self‐Powered Biosensing

The growing power demands of wearable electronic devices have stimulated the development of on‐body energy‐harvesting strategies. This article reviews the recent progress on rapidly emerging wearable biofuel cells (BFCs), along with related challenges and prospects. Advanced on‐body BFCs in various wearable platforms, e.g., textiles, patches, temporary tattoo, or contact lenses, enable attractive advantages for bioenergy harnessing and self‐powered biosensing. These noninvasive BFCs open up unique opportunities for utilizing bioenergy or monitoring biomarkers present in biofluids, e.g., sweat, saliva, interstitial fluid, and tears, toward new biomedical, fitness, or defense applications. However, the realization of effective wearable BFC requires high‐quality enzyme‐electronic interface with efficient enzymatic and electrochemical processes and mechanical flexibility. Understanding the kinetics and mechanisms involved in the electron transfer process, as well as enzyme immobilization techniques, is essential for efficient and stable bioenergy harvesting under diverse mechanical strains and changing operational conditions expected in different biofluids and in a variety of outdoor activities. These key challenges of wearable BFCs are discussed along with potential solutions and future prospects. Understanding these obstacles and opportunities is crucial for transforming traditional bench‐top BFCs to effective and successful wearable BFCs.     

Synthesis and Characterization of 2D Molybdenum Carbide (MXene)

Large scale synthesis and delamination of 2D Mo₂CT _x (where T is a surface termination group) has been achieved by selectively etching gallium from the recently discovered nanolaminated, ternary transition metal carbide Mo₂Ga₂C. Different synthesis and delamination routes result in different flake morphologies. The resistivity of free‐standing Mo₂CT _x films increases by an order of magnitude as the temperature is reduced from 300 to 10 K, suggesting semiconductor‐like behavior of this MXene, in contrast to Ti₃C₂T _x which exhibits metallic behavior. At 10 K, the magnetoresistance is positive. Additionally, changes in electronic transport are observed upon annealing of the films. When 2 μm thick films are tested as electrodes in supercapacitors, capacitances as high as 700 F cm^?3 in a 1 m sulfuric acid electrolyte and high capacity retention for at least 10,000 cycles at 10 A g^?1 are obtained. Free‐standing Mo₂CT _x films, with ≈8 wt% carbon nanotubes, perform well when tested as an electrode material for Li‐ions, especially at high rates. At 20 and 131 C cycling rates, stable reversible capacities of 250 and 76 mAh g^?1, respectively, are achieved for over 1000 cycles.     

下一代手机设计的集成技术

为了满足对于高度集成的无线电半导体解决方案日益增长的需求，蜂窝电话的技术创新正在沿着两个不同的方向发展。其一是在芯片级和封装级的功能集成，其二是完整的半导体系统解决方案。后者将所有必须的芯片、模块和软件捆绑到一个全面的解决方案中，从而简化了设计步骤．加快了产品进入市场的时间。     

MPPA光动力作用诱导人鼻咽癌细胞凋亡的实验研究

为观察MPPa光动力作用对鼻咽癌细胞凋亡的影响,应用AnnexinV—PI双染结合流式细胞仪分析MPPa光动力作用后人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率。结果显示MPPa光动力作用实验组人鼻咽癌细胞株CNE2细胞发生凋亡和继发性坏死的比率分别增加到16.43 %和4.64 % ,且均显著高于单纯光照射组、单纯MMPa光敏剂处理组和假照射组(P <0 .0 1) ,而三对照组间无明显差异(P >0 .0 5 )。表明MPPa光动力作用能有效诱导低分化人鼻咽癌细胞株CNE2细胞凋亡的发生。这也可能是MPPa光动力作用杀伤鼻咽癌的重要机制之一。