Velocity measurements inside the concentration boundary layer during copper-magneto-electrolysis using a novel laser Doppler profile sensor

The evolution of the velocity boundary layer during the initial phase of copper electrolysis under the influence of a magnetic field is studied by using particle image velocimetry and a novel laser Doppler velocity profile sensor. With this new sensor, time-resolved velocity measurements within 400 μm of a vertically aligned cathode in an aqueous 0.05 M CuSO₄-solution are presented. In this way, the complex interaction of Lorentz force and opposing buoyancy-driven convection was studied by measuring the resulting velocity profile inside the concentration boundary layer with a spatial resolution of 15 μm. It is shown that the Lorentz force-driven convection only dominates the velocity boundary layer during the early phase of electrolysis and induces a linear velocity profile near the cathode. The linear relationship between the velocity gradient and Lorentz force is determined. With the onset of the opposing buoyancy-driven convection at the cathode, a duplex structure of the boundary layer appears. Its characteristic quantities, given by the horizontal distances, δ_max and δv=0, where the velocity reaches the maximum and where it is equal to zero, remain nearly unchanged, while the maximum velocity, vmax, in spite of the counteracting Lorentz force, increases faster as compared to pure natural convection, depending on the current density.     

Volumetric analysis of MRI data monitoring the treatment of polycystic kidney disease in a mouse model

Object  

The human condition autosomal dominant polycystic kidney disease (ADPKD) is characterized by the growth of cysts in the kidneys that increase renal volume and lead to kidney failure. Mice studies are performed for treatment development monitored with imaging. The analysis of the imaging data is typically manual, which is costly and potentially biased. This paper presents a reliable and reproducible method for the automated segmentation of polycystic mouse kidneys.     

A Comprehensive Review of Genetically Engineered Mouse Models for Prader-Willi Syndrome Research

Prader-Willi syndrome (PWS) is a neurogenetic multifactorial disorder caused by the deletion or inactivation of paternally imprinted genes on human chromosome 15q11-q13. The affected homologous locus is on mouse chromosome 7C. The positional conservation and organization of genes including the imprinting pattern between mice and men implies similar physiological functions of this locus. Therefore, considerable efforts to recreate the pathogenesis of PWS have been accomplished in mouse models. We provide a summary of different mouse models that were generated for the analysis of PWS and discuss their impact on our current understanding of corresponding genes, their putative functions and the pathogenesis of PWS. Murine models of PWS unveiled the contribution of each affected gene to this multi-facetted disease, and also enabled the establishment of the minimal critical genomic region (PWScr) responsible for core symptoms, highlighting the importance of non-protein coding genes in the PWS locus. Although the underlying disease-causing mechanisms of PWS remain widely unresolved and existing mouse models do not fully capture the entire spectrum of the human PWS disorder, continuous improvements of genetically engineered mouse models have proven to be very powerful and valuable tools in PWS research.     

Targeted Synthesis of Complex Spiro[3H-indole-3,2′-pyrrolidin]-2(1H)-ones by Intramolecular Cyclization of Azomethine Ylides: Highly Potent MDM2–p53 Inhibitors

Mouse double minute 2 (MDM2) is a main and direct inhibitor of the crucial tumor suppressor p53. Reports from initial clinical trials showed that blocking this interaction with a small-molecule inhibitor can have great value in the treatment of cancer for patients with p53 wild-type tumors; however, it also revealed dose-limiting hematological toxicities and drug-induced resistance as main issues. To overcome the former, an inhibitor with superior potency and pharmacokinetic properties to ultimately achieve full efficacy with less-frequent dosing schedules is required. Toward this aim, we optimized our recently reported spiro-oxindole inhibitors by focusing on the crucial interaction with the amino acid side chain of His96_MDM2. The designed molecules required the targeted synthesis of structurally complex spiro[indole-3,2′-pyrrolo[2,3-c]pyrrole]-2,4′-diones for which we developed an unprecedented intramolecular azomethine ylide cycloaddition and investigated the results by computational methods. One of the new compounds showed superior cellular potency over previously reported BI-0252. This finding is a significant step toward an inhibitor suitable to potentially mitigate hematological on-target adverse effects.     

Glass formation and mechanical properties of (Cu50Zr50)100−xAlx (x = 0, 4, 5, 7) bulk metallic glasses

The effect of Al content on the glass formation and mechanical properties was studied for (Cu₅₀Zr₅₀)_100−xAl_x (x = 0, 4, 5, 7) bulk metallic glasses. The crystallization temperatures of fully amorphous Cu₅₀Zr₅₀, Cu₄₈Zr₄₈Al₄, Cu_47.5Zr_47.5Al₅ and Cu_46.5Zr_46.5Al₇ as-cast rods are 724 K, 753 K, 758 K and 782 K, respectively. The mechanical properties were investigated under compression at room temperature. As-cast Cu_46.5Zr_46.5Al₇ shows the highest yield strength (1867 MPa), whereas Cu_47.5Zr_47.5Al₅ shows the largest fracture strain of 11.2%. The fracture surfaces of the compressed samples were investigated by scanning electron microscopy and their morphology has been correlated with the compressive plasticity.     

Development of a multifunctional coating system for laser-induced material transport

The aim of our research is to develop a novel surface coating for the use in laser microdissection and laser pressure catapulting (LMPC). LMPC is a contact- and contamination-free technique to separate histologic material and living cells for further proteomic and genomic analysis. Several physico-chemical functions must be included within the optimum coating system designed for this purpose, like optical absorption at the laser wavelength, combined with optical transparency in the visible region, a control of the laser ablation process, mechanical stability and biocompability for the adhesion of the histologic material.To achieve the optimum system the combination of several layers is required. The optical absorbance to capture the radiation energy from a frequency-tripled Nd:YAG laser (λ = 355 nm) is reached by a thin layer of zinc oxide (ZnO), deposited by hollow cathode gas flow sputtering. The laser ablation process is controlled by a polyelectrolyte multilayer, consisting of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS). The evaporation of chemisorbed water from the film is used to promote the catapulting process. For the mechanically stable, laser-dissectible layer organic coatings, like photoresists or lacquers, are suitable. Silica-containing polyacrylate nanocomposites were employed for this purpose.The investigation of the coating system included LMPC experiments with varying compositions of the layer system. The best results were obtained using a system consisting of ZnO, a polyelectrolyte multilayer deposited from 0.1 M Na₂SO₄ containing polymer solutions, and a 1.5-μm thick layer of the polyacrylate nanocomposite.To check the quality of the developed system, experiments with the commonly used poly(ethylene naphthalate) (PEN) foil were performed simultaneously. In addition to the determination of the parameters required for LMPC, quantitative real-time Polymerase Chain Reaction (rt-PCR) of the dissected material verified the benefit of the new system.     

Untersuchungen des Zeitstandverhaltens von Schweißverbindungen der Werkstoffe X 20 CrMoV 12 1 und GS-17 CrMoV 5 11

Untersucht wird das Zeitstandverhalten gleichartiger Schweißverbindungen des Werkstoffs X 20 CrMoV 12 1 sowie ungleichartiger Verbindungen zwischen diesem und dem Werkstoff GS-17 CrMoV 5 11. Bei quer entnommenen Kleinproben zeigt sich ein Abfall der Zeitstandfestigkeit gegenüber dem Grundwerkstoff. Große Querproben aus einer ungleichartigen Verbindung weisen eine höhere Zeitstandfestigkeit auf als entsprechende Kleinproben. Zur Klärung der in den Schweißverbindungen auftretenden Spannungs- und Dehnungsumlagerungen wurden inelastische Finite-Elemente-(FE-)Berechnungen durchgeführt. Dabei wurde das Zeitdehnverhalten von Grundwerkstoffen, Schweißgut und thermisch simulierten Gefügen der Wärmeeinflußzone berücksichtigt. Die Anwendung spannungsbezogener Kriterien zur Bestimmung der Anrißdauer der Proben auf die FE-Ergebnisse läßt eine Voraussage des Versagensorts sowie eine sichere Abschätzung der Versagenszeit zu.     

Teachers' perceptions of students' reading abilities: An examination of the relationship between teachers' judgments and students' performance across a continuum of rating methods.

Teacher perceptions about students' academic abilities are important for several reasons (e.g., instructional decision making, special education entitlement decisions). Not surprisingly, researchers have investigated the accuracy of teachers' decisions. Although some data reveal that teachers are relatively good judges of academic performance, other findings have suggested otherwise. A likely explanation for conflicting findings is the varying assessment methods (e.g., direct vs. indirect, norm-referenced vs. peer-independent) and different data analysis procedures that have been used across studies. The purpose of this study was to investigate a continuum of teacher-perception assessment methods as they corresponded to students' oral reading fluency performance. Participants included 10 teachers and 87 first, second, and third grade students from a suburban school in the northeast. Overall results suggested that teachers were generally accurate when estimating students' performance when students had strong oral reading fluency skills, but teachers had more difficulty judging students with average to low oral reading fluency. Further, data interpretation of teachers' judgment accuracy differed somewhat depending upon the statistical method employed. Implications, limitations, and suggestions for future research related to this study are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)