重复压裂气井产能模拟研究

由于重复压裂气井原有裂缝的失效程度难以评价。导致重复压裂气井的产能预测常常有很大的误差。为此，根据重复压裂气井的压前产量和舍水率，拟合了原有裂缝的有效率，在考虑新裂缝和原有裂缝共同作用的情况下，建立了气、水两相平面二维的裂缝-油藏数学模型，通过对模型的数值求解，对重复压裂气井进行了产能评价。研究表明，在考虑新、老裂缝共同作用下的产能评价方法，能更为准确地预测重复压裂后的生产动态情况，对压裂时机的确定、压后经济效益的预测都具有重要的指导意义。     

塔里木盆地雅—轮地区断层封堵性研究

塔里木盆地雅—轮地区大部分圈闭及油气藏的形成与断裂作用密切相关,对断层封堵性的综合研究已成为该地区油气勘探的重要因素。应用地质定性分析、Allen剖面分析及断层涂抹量计算等方法,通过对研究区4个典型断层封堵油气藏的系统解剖研究,建立雅—轮地区断裂封堵性评价标准,对雅—轮地区断层的封堵性进行综合评价。雅—轮地区断层整体封堵效果良好,断层在圈闭形成和油气聚集过程中起着重要的控制作用,形成一系列沿断层带状分布的油气藏。亚南断裂的封堵效果东部要优于西部,下部要优于上部。轮台断裂在不同部位、不同深度均存在着不同的封堵性能,封堵非均质性明显,总体显示断裂东部地区(也根附近)的封堵性要好于西部雅南地区。      

聚丙烯酰胺/氧化石墨纳米复合材料的研究

氧化石墨具有良好的层状结构，其层间具有丰富的官能团，能与有机聚合物形成插层纳米复合材料进而改善材料的性能．采用层离吸收-原位聚合法制备了聚丙烯酰胺／氧化石墨纳米复合材料，并采用XRD、HREM及DSC等对其结构和性能进行了表征。结果表明，聚丙烯酰胺与氧化石墨两者之间存在着较强的相互作用，材料的玻璃化转变温度得到提高，层离吸收-原位聚合法是获得聚丙烯酰胺／氧化石墨层纳米复合材料的有效途径，聚丙烯酰胺在氧化石墨中存在着多种排列方式，不同层间距(1．6nm和2．8nm)的聚丙烯酰胺／氧化石墨纳米复合结构同时存在。     

二氧化锡溅射工艺研究

SnO2 是最早使用也是目前使用最广泛的一种气敏材料 ,使用该材料设计制作的气敏传感器具有许多优点。在简要介绍溅射镀膜的成膜过程和特点的基础上 ,着重介绍了SnO2 膜的制备流程 ,分析了功率和温度变化对成膜质量的影响     

绚丽多彩的楚汉丝织品（之二）──色彩与纹饰

湖南出土的楚汉丝织品，以马王堆一、三号汉墓所出为代表，其色彩反映了我国当时印染工艺技术所达到的高度水平，其纹饰则显示了鲜明的时代特征，表现了运动、气势和古拙的艺术风格，充满着神奇浪漫的色彩。     

Effect of alendronate treatment on the osteoclastogenic potential of bone marrow cells in mice

The expression of cytokeratins (CK), involucrin, vimentin, CD34, and alpha-smooth-muscle actin was studied in fetal and adult hair follicles. The first stage of the developing hair follicle is characterized by palisaded, elongated epithelial cells budding from the epidermal basal layer. These cells express CK5/6, CK14, CK17, CK19, and vimentin. During the following weeks of gestation, different structures in the developing hair follicle can be identified and characterized. The matrical cells display only CK19. The keratinocytes of the outer root sheath express CK5/6, CK14, CK17, CK19, and involucrin; those of the inner root sheath, CK4, CK18, and involucrin; those of the isthmus, the same profile as the ORS. In the infundibulum, the basal-layer keratinocytes express CK5/6, CK14, CK17, and CK19, whereas in the suprabasal layers CK1, CK4, CK10, CK14, and CK17 are seen. The adult hair follicle in anagen fails to express CK19 in the matrical cells and isthmus and both CK17 and CK19 in the infundibulum. These profiles of intermediate filaments and other markers appear to be potentially useful in categorizing neoplasms with apparent follicular differentiation.     

Purification of the cardiac sarcoplasmic reticulum membrane protein phospholamban from recombinant Escherichia coli

Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification.