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排序方式: 共有600条查询结果,搜索用时 31 毫秒
11.
Juskanič Dominik Mištinová Jana Poláková Hollý Samuel Sekerešová Monika Koleják Kamil Pátrovič Lukáš 《Magma (New York, N.Y.)》2022,35(1):45-52
Magnetic Resonance Materials in Physics, Biology and Medicine - Oncometabolite D-2-hydroxyglutarate (2HG) is pooled in isocitrate dehydrogenase (IDH)-mutant glioma cells. Detecting 2HG by MR... 相似文献
12.
Khondakar Kamil Reza Jing Wang Ramanathan Vaidyanathan Shuvashis Dey Yuling Wang Matt Trau 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(9)
Cancer diagnosis and patient monitoring require sensitive and simultaneous measurement of multiple cancer biomarkers considering that single biomarker analysis present inadequate information on the underlying biological transformations. Thus, development of sensitive and selective assays for multiple biomarker detection might improve clinical diagnosis and expedite the treatment process. Herein, a microfluidic platform for the rapid, sensitive, and parallel detection of multiple cancer‐specific protein biomarkers from complex biological samples is presented. This approach utilizes alternating current electrohydrodynamic‐induced surface shear forces that provide exquisite control over fluid flow thereby enhancing target–sensor interactions and minimizing non‐specific binding. Further, the use of surface‐enhanced Raman scattering‐based spectral encoding with individual barcodes for different targets enables specific and simultaneous detection of captured protein biomarkers. Using this approach, the specific and sensitive detection of clinically relevant biomarkers including human epidermal growth factor receptor 2 (HER2); Mucin 1, cell surface associated (MUC1); epidermal growth factor receptor; and Mucin 16, cell surface associated (MUC16) at concentrations as low as 10 fg mL?1 in patient serum is demonstrated. Successful target detection from patient samples further demonstrates the potential of this current approach for the clinical diagnosis, which envisages a clinical translation for a rapid and sensitive appraisal of clinical samples in cancer diagnostics. 相似文献
13.
A new measurement technique for the characterization of uniaxial as well as biaxial anisotropic surfaces and thin films is introduced. This technique is based on perpendicular-incidence photometric ellipsometry, in which a spectral-photometric dynamic ellipsometer with a rotating polarizer is used. This method is sensitive, contactless, nondestructive, and efficient for the estimation of anisotropic behavior. Furthermore, the spectroscopic measurement directly provides the anisotropy dispersion down to the UV wavelength range. Results on structurally anisotropic HfO(2) coatings are presented. 相似文献
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分析了特征选择采用互信息方法时文本分类性能较低的原因,认为与其在特征选择时倾向于选择稀有特征这一缺陷有很大关系。在此基础上,提出了一种基于分散度和平均频度的互信息特征选择方法。实验结果表明,改进后的互信息方法使文本分类性能有明显提高。 相似文献
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Carbon fibre reinforced ceramic matrix composites (CMC), originally developed for lightweight heat shields of spacecraft, are used for high performance brake discs in sports cars from different manufacturers. In contrast to the CMC materials for space applications, based on woven fabrics and costly manufacturing methods, these low cost friction materials are produced by liquid silicon infiltration of porous Carbon/Carbon (C/C) preforms, based on short fibre reinforced CFRP green bodies manufactured via warm press technique. In this work, different manufacturing methods for ventilated CMC brake discs are compared to each other, and the development of a new technology for the manufacture of single piece C/C‐SiC brake discs in net shape technique is presented. 相似文献
18.
Josef ajka Tom Dostl Kamil Vrba 《International Journal of Circuit Theory and Applications》2004,32(3):133-138
We propose a new current conveyor terminology and explain how these terms are coined. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
19.
With the deployment of native multicast in commercial networks, multicast is getting closer to becoming a ubiquitous service in the Internet. The success of this deployment largely depends on the availability of good management tools and systems. One of the most important management tasks for multicast is to verify the availability of the service to its users. This task is usually referred to as reachability monitoring. Reachability monitoring requires a number of monitoring stations to work together to collect this information in a distributed manner in the interdomain scale. In this paper we present a general architecture for multicast reachability monitoring systems and focus on three critical functions: agent configuration, monitoring, and feedback collection. For each component, we provide a number of alternative approaches to implement the required functionality and discuss their advantages and disadvantages. Then, we focus on the feedback collection component. To a large extent, it determines the complexity and the overhead of a monitoring system. Using simulations, we compare a number of alternative approaches for feedback collection and make suggestions on when to use each. We believe our work provides insight into the issues and considerations in designing and developing multicast reachability monitoring systems. 相似文献
20.
Zinc levels in serum and/or tissue are reported to be altered in melanoma with unknown effects on melanoma development and biology. The purpose of this study was to examine the effects of acute chelation of free intracellular zinc pools in melanoma cell lines Bowes and A375, as well as selected melanoma tissue explants with high or low intracellular free zinc. Zinc chelating agent TPEN at the concentration of 25 µM was employed during 48 h, which significantly reduced intracellular free zinc while decreasing melanoma cell proliferation, inducing G1/S arrest and cell damage leading to mitochondrial, caspase-dependent apoptosis. Chelation of free zinc was also associated with increased generation of superoxide in cell lines but not marked lysosomal membrane damage. Conversely, melanoma explant cultures mostly displayed time-dependent loss of lysosomal membrane integrity in the presence of slowly growing superoxide levels. Loss of free zinc-dependent p53 activity was similarly disparate in individual melanoma models. Surviving melanoma cells were arrested in the cell cycle, and varying proportions of them exhibited features characteristic of premature senescence, which increased in time despite zinc reloading. The present results show that melanoma cells with varying free zinc levels respond to its acute loss in a number of individual ways, reflecting activated mechanisms including oxidative stress, lysosomal damage, and p53 activity leading to heterogenous outcomes including cell death, transient, and/or permanent cell cycle arrest and premature senescence. 相似文献