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排序方式: 共有1406条查询结果,搜索用时 15 毫秒
51.
Dr. Matthias Hauf Dr. Florian Richter Tobias Schneider Thomas Faidt Dr. Berta M. Martins Dr. Tobias Baumann Dr. Patrick Durkin Prof. Dr. Holger Dobbek Prof. Dr. Karin Jacobs Prof. Dr. Andreas Möglich Prof. Dr. Nediljko Budisa 《Chembiochem : a European journal of chemical biology》2017,18(18):1771-1771
52.
Application of β-Sitosterol + γ-Oryzanol-Structured Organogel as Migration Barrier in Filled Chocolate Products 下载免费PDF全文
Alexandra Wendt Karin Abraham Christin Wernecke Julia Pfeiffer Eckhard Flöter 《Journal of the American Oil Chemists' Society》2017,94(9):1131-1140
Oil migration in filled pralines is a phenomenon that is highly correlated with the occurrence of chocolate bloom. In this study the potential to suppress or prevent oil migration by incorporation of sterol/sterolster-structured organogels was evaluated. Different quantities, 2.5 or 14% (w/w), of gel with structurant levels of either 10 or 25% (w/w) were studied in a layered model system. The gel was either a part of the nougat or of the chocolate phase, or as a separate layer. Samples were monitored regularly for a period of 24 weeks at storage temperatures of 10, 18 and 28 °C. The amount of migrated oil was determined via DSC analysis of a surface sample. The results indicate that, despite the additional oil brought into the system via the oleogel, the level of oil found in the chocolate layer is reduced through the presence of the gel. In particular, the three-layer system and gelled chocolate appear to be promising routes to either suppress oil migration or improve nutritional profiles by incorporation of liquid oils. 相似文献
53.
Zako T Sakono M Kobayashi T Sörgjerd K Nilsson KP Hammarström P Lindgren M Maeda M 《Chembiochem : a European journal of chemical biology》2012,13(3):358-363
Needles and noodles: Studying amyloid toxicity is important for understanding protein misfolding diseases. Using a luminescent conjugated polythiophene, we found that cell binding of nontoxic filamentous amyloids of insulin and β2-microglobulin was less efficient than that of toxic fibrillar amyloids; this suggests a correlation between amyloid toxicity and cell binding. 相似文献
54.
55.
The Phospholipid Composition of Kangaroo Spermatozoa Verified by Mass Spectrometric Lipid Analysis 下载免费PDF全文
Kathrin?M.?EngelEmail author Jürgen?Schiller Karin?Müller Dirk?Dannenberger Ulrike?Jakop 《Lipids》2017,52(10):857-869
Cryopreservation of kangaroo sperm has not been successful so far, and yet there is no promising cryopreservation protocol for these cells available. However, conservation of gametes is extremely important, particularly in the context of preserving endangered species. As spermatozoa are comprised of different membrane systems, the composition of these membranes might account for difficulties in cryopreservation. Lipids, as the main components, affect the physical properties of biological membranes and play a major role in sperm maturation. Therefore, knowledge of the lipid composition is crucial for any further step toward the preservation of the species. We used MALDI‐TOF, ESI‐IT, tandem mass spectrometry, and thin layer chromatography to investigate the lipid composition of epididymal spermatozoa of four different kangaroo species. Spectra of these species were very similar with respect to the identified lipid species. Tremendous changes in the lipid composition during the transit of sperm from caput to cauda epididymis could be seen, specifically an increase in poly‐unsaturated fatty acids, ether lipids, and plasmalogens, as well as a reduction in mono‐ and di‐unsaturated fatty acids. Additionally, phosphatidylcholines containing docosatrienoic acid (22:3), a heretofore unknown fatty acid for sperm membranes, showed the highest abundance in kangaroo sperm. 相似文献
56.
Dr. Alexander Fries Dr. Laura S. Mazzaferro Dr. Björn Grüning Dr. Philippe Bisel Karin Stibal Patrick C. F. Buchholz Prof. Dr. Jürgen Pleiss Prof. Dr. Georg A. Sprenger Prof. Dr. Michael Müller 《Chembiochem : a European journal of chemical biology》2019,20(13):1672-1677
Chorismate and isochorismate constitute branch-point intermediates in the biosynthesis of many aromatic metabolites in microorganisms and plants. To obtain unnatural compounds, we modified the route to menaquinone in Escherichia coli. We propose a model for the binding of isochorismate to the active site of MenD ((1R,2S, 5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxycyclohex-3-ene-1-carboxylate (SEPHCHC) synthase) that explains the outcome of the native reaction with α-ketoglutarate. We have rationally designed variants of MenD for the conversion of several isochorismate analogues. The double-variant Asn117Arg–Leu478Thr preferentially converts (5S,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylate (2,3-trans-CHD), the hydrolysis product of isochorismate, with a >70-fold higher ratio than that for the wild type. The single-variant Arg107Ile uses (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylate (2,3-trans-CHA) as substrate with >6-fold conversion compared to wild-type MenD. The novel compounds have been made accessible in vivo (up to 5.3 g L−1). Unexpectedly, as the identified residues such as Arg107 are highly conserved (>94 %), some of the designed variations can be found in wild-type SEPHCHC synthases from other bacteria (Arg107Lys, 0.3 %). This raises the question for the possible natural occurrence of as yet unexplored branches of the shikimate pathway. 相似文献
57.
Dr. Fumika Karaki Sho Umemoto Karin Ashizawa Tomoya Oki Noriko Sato Takumi Ogino Naoto Ishibashi Ryoto Someya Dr. Kanako Miyano Dr. Shigeto Hirayama Prof. Yasuhito Uezono Prof. Hideaki Fujii 《ChemMedChem》2019,14(21):1840-1848
Although the advantages of sp3-rich, sterically complicated molecules in drug development have been pointed out, modern screening libraries are filled with planar, sp2-rich components. Compounds that are sp3-rich are difficult to synthesize, and thus we aimed to invent an efficient method to construct sp3-rich libraries. By modifying sp3-rich 7-azanorbornane scaffolds through click chemistry, we efficiently prepared a small set of compounds. These compounds were not only sp3-rich, but also had sufficient “lead-like” properties in view of molecular weights and hydrophobicity. Screening assays of this library provided weak κ opioid receptor agonists and growth hormone secretagogue receptor agonists with high hit rates. These results indicate that the 7-azanorbornane scaffold may be a “privileged structure” for lead identification in drug discovery. 相似文献
58.
S. Mariccor A. B. Batoy Sabine Borgmann Karin Flick Josephine Griffith Jeffrey J. Jones Viswanathan Saraswathi Alyssa H. Hasty Peter Kaiser Charles L. Wilkins 《Lipids》2009,44(4):367-371
Here we describe a study of the feasibility of lipid and phospholipid (PL) profiling using matrix assisted laser desorption/ionization
(MALDI) Fourier transform mass spectrometry (FTMS) for two different applications. In this work PL profiles of different mammalian
tissues as well as those of whole cell organisms were examined. In particular, comparative analysis of lipid and PL profiles
of tissues from mice fed different diets was done and, in another application, MALDI FTMS was used to analyze PL profiles
of genetically modified Saccharomyces cerevisiae. Computational sorting of the observed ions was done in order to group the lipid and PL ions from complex MALDI spectra.
The PL profiles of liver tissues from mice fed different diets showed a cross correlation coefficient of 0.2580, indicating
significant dissimilarity, and revealed more than 30 significantly different peaks at the 99.9% confidence level. Histogram
plots derived from the spectra of wild type and genetically modified yeast resulted in a cross correlation coefficient 0.8941
showing greater similarity, but still revealing a number of significantly different peaks. Based on these results, it appears
possible to use MALDI FTMS to identify PLs as potential biomarkers for metabolic processes in whole cells and tissues. 相似文献
59.
Cleavable surfactants 总被引:6,自引:0,他引:6
Per-Erik Hellberg Karin Bergström Krister Holmberg 《Journal of surfactants and detergents》2000,3(1):81-91
Cleavable surfactants are of interest for several reasons. Above all, the development of surfactants with weak bonds deliberately built into the structure is driven by the need for improved biodegradability of amphiphiles. The breakdown may be catalyzed by enzymes, and biodegradation would be the normal mechanism in sewage plants. Alternatively, the surfactant may degrade by chemical means, e.g., induced by acid, alkali, ultraviolet (UV) light, heat, or ozone. Acid- and alkali-labile surfactants have attracted particular attention, and there is often a compromise between required stability at one stage and ease of breakdown at a subsequent stage. The paper reviews the main routes used to prepare cleavable surfactants and points out advantages and disadvantages of the different approaches. Emphasis is placed on the development during recent years. Cyclic and acyclic acetals, ketals, and ortho esters are the most important types of bonds for the preparation of acid-labile surfactants, whereas alkali-labile amphiphiles usually are based on ester bonds. The ester bond approach has been particularly important for cationic surfactants, and so-called ester quats have rapidly taken a large share of the traditional market for quats. Betaine esters constitute a special class of ester with very pronounced pH dependence. UV-labile surfactants based, for instance, on an azo bond, offer promise for the future. 相似文献
60.
Colour research from different scientific traditions start from different basic questions and use different methods and concepts. This makes it difficult to communicate and to judge result relevance in a wider perspective. Here we start from architects' need of colour knowledge and discuss recent studies of colour appearance and colour emotion, with and without explicit connection to architecture. We stress the need for further development and clarification of concepts and conclude that the multitude of studies with different approaches can be seen as cases, jointly adding to a widened and deepened understanding of colour. © 2010 Wiley Periodicals, Inc. Col Res Appl, 2010 相似文献