Designing a 21st and 22nd century fibre broadband access network

DSL technology has been incredibly successful at allowing copper telephony wires to deliver broadband services. However, the physics of copper dictate that as speeds increase further it will be necessary to push optical fibre closer to the customer — to the cabinet, and ultimately to the premises. Deploying optical fibre to the mass consumer is well known as representing a significant capital investment and will take many years to deploy. It would be fair to say that putting fibre in the residential access network is a ‘once in a century’ investment. There are many different choices of ‘high fibre’ broadband access systems and as technology develops further, this situation is likely to continue. When operators deploy fibre in the access network, it is therefore vital that they do so in a future-proof manner which can support technologies and architectures yet to come, and allow migration and further evolution.     

Complete Monte Carlo RF analysis of “real”short-channel compound FET's

A comprehensive RF analysis technique based on ensemble Monte Carlo (EMC) simulation of compound FET's with realistic device geometry is presented. Y-parameters are obtained through Fourier transformation of the EMC transients in response to small changes in the terminal voltages. The terminal currents are statistically enhanced and filtered to allow for reliable y-parameters extraction. Improved analytic procedure for extracting the intrinsic device small-signal circuit components is described. As a result, stable y-parameters and reliable circuit components can he extracted for the whole range of device operation voltages. Parasitic components like contact and gate resistances are included in the y-parameters at a post processing stage to facilitate the forecast of the performance figures of merit of real devices. The developed RF technique has been applied in the EMC simulation of pseudomorphic HEMT's (pHEMT's) fabricated at the Glasgow Nanoelectronics Research Center. Good agreement has been achieved between the simulated and measured small-signal circuit components and performance figures of merit     

Combined bioreaction and separation in centrifugal fields using zonal rotors

Combined biosynthesis and product separation has been successfully performed for the first time using a zonal centrifugal bioreactor–separator. The biosynthesis of dextran polymer from sucrose using the dextransucrase enzyme was investigated in order to evaluate bioreactor performance. The bioreactor consisted of a bowl, fitted with a Reorienting gradient rotor which facilitated substrate and enzyme loading and the reproducible unloading of solutions from the system. This allowed the distribution of substrate, enzyme and product materials in the bowl at the end of each trial to be accurately determined. Studies have indicated that combined bioreaction–separation is possible using this system. However, viscosity build-up in the bioreactor must be minimised in order to increase the yield of polymer product per unit time and improve product separation.     

Security of capsular fixation: small-versus large-hole plate-hepatic lenses

PURPOSE: To assess the effect of relatively large positioning holes on the security of capsular bag fixation of plate-haptic silicone intraocular lenses (IOLs). SETTING: Center for Research on Ocular Therapeutics and Biodevices, Department of Ophthalmology, Storm Eye Institute, Medical University of South Carolina, Charleston, South Carolina, USA. METHODS: This study tested the hypothesis that larger holes allow ingrowth of lens material, fibrous tissue, or both through them, which helps fixate the lens more firmly in the capsular bag. Five rabbits had bilateral continuous curvilinear capsulorhexis, phacoemulsification, and implantation of a plate-haptic silicone IOL. An IOL with a small, round positioning hole (Staar AA-4203V) was implanted in the right eye in each rabbit, and a large-hole IOL (Staar AA-4203VF) was implanted in the left eye. After 2 months, all rabbits were killed. The force required to extract one haptic from the capsular bag was measured with a digital force meter. All eyes had histopathological analysis. RESULTS: It was slightly more difficult to extract a large-hole IOL from the capsular bag, although this trend was not statistically significant. However, histopathological analysis consistently showed 360 degree synechia formation through the holes, showing that the IOL could be securely fixed in position. CONCLUSIONS: Proliferation of lens epithelial cells through a large positioning hole in a plate-haptic silicone IOL may improve the long-term security of capsular bag fixation. This will help reduce the incidence of IOL decentration and dislocation.     

Continuous annular chromatography for the separation of beet molasses

The continuous separation of a multicomponent feedstock on a rotating annular chromatograph is described. The annulus width was 12 mm, diameter 300 mm and height 1400 mm. The annulus was packed with a 420 micron ion-exchange resin in the sodium form. The feedstock separated was beet molasses which is the final syrup spun off after repeated crystallisations in the extraction of sugar. Two basic mechanisms were in operation to separate sucrose from impurities: ion exclusion and molecular sieving. A statistical approach was made to set up an experimental programme and analyse the results. Four factors were studied: feed flowrate, feed concentration, annulus rotation rate and eluent rate. Two of these, namely feed rate and eluent rate, significantly affected the response. The response was the separation achieved using the annular chromatograph between the sucrose and non-sucrose solids. A further series of experiments demonstrated the practical considerations to be made when separating beet molasses. A crystallisable product sucrose rate of 207 gh^?1 was achieved.     

Recombinant antigens for specific and sensitive serodiagnosis of Latin American tegumentary leishmaniasis

The diagnostic potential of recombinant leishmanial antigens for Latin American tegumentary leishmaniasis (LATL) was examined. Two Leishmania (Viannia) peruviana recombinant proteins, T26-U2 and T26-U4, were assessed by their reactivity to detect specific anti-leishmanial antibodies. Seventy-eight individual sera from persons with LATL, 39 from those with other diseases, and 10 negative control sera were tested by Western blotting and enzyme-linked immunosorbent assay. The sensitivity of the test using T26-U2 plus T26-U4 was similar to that obtained with whole parasite extract (92%). However, the specificity obtained using both recombinant antigens (87%) was higher than that of the whole parasite extract (65%). All tests using recombinant proteins (T26-U2, T26-U2 plus T26-U4 or T26-U4) had a higher positive predictive value (89%, 92% and 98%, respectively) than the value obtained using total parasites (81%). Eleven Colombian sera were also tested, and the results indicated that T26-U2 plus T26-U4 could be used successfully in Peru and in other Latin American countries.     

Isolation and physicochemical characterization of the half-unit membranes of oilseed lipid bodies

The lipid-free residue of lipid body membranes was isolated from quiescent peanuts and was physicochemically characterized. The preponderant component of the residue was proteinaceous and consisted of at least two polypeptides according to ultracentrifugation, gel filtration, gel electrophoresis and HPLC. The molecular weight of the principal polypeptide was between 10,000 and 16,000 daltons. Only one antigen, immunochemically unique with respect to other peanut components, was detected. Spectral analyses indicated the presence of a protoheme and revealed conformational modes of β-sheet and unordered structure but no α-helix. The amino acid composition was similar to that of an integral membrane polypeptide rather than to those of peripheral membranes or other plant polypeptides. The hydrophobicity, conformation and quantitative content of polypeptides were suitable for the existence of a monolayer at the lipid body-cytoplasm interface. The results indicated that lipid body coatings physicochemically resemble membranes of intracellular organelles and supported the morphological concept that the coatings are half-unit biological membranes. Reutilization of lipid body membranes appeared possible after lipid depletion during seed germination.     

Research issues in developing compact pulsed power for high peak power applications on mobile platforms

Pulsed power is a technology that is suited to drive electrical loads requiring very large power pulses in short bursts (high-peak power). Certain applications require technology that can be deployed in small spaces under stressful environments, e.g., on a ship, vehicle, or aircraft. In 2001, the U.S. Department of Defense (DoD) launched a long-range (five-year) Multidisciplinary University Research Initiative (MURI) to study fundamental issues for compact pulsed power. This research program is endeavoring to: 1) introduce new materials for use in pulsed power systems; 2) examine alternative topologies for compact pulse generation; 3) study pulsed power switches, including pseudospark switches; and 4) investigate the basic physics related to the generation of pulsed power, such as the behavior of liquid dielectrics under intense electric field conditions. Furthermore, the integration of all of these building blocks is impacted by system architecture (how things are put together). This paper reviews the advances put forth to date by the researchers in this program and will assess the potential impact for future development of compact pulsed power systems.     

Sensory characterization of human milk

Fore and hind milks from a single morning feed collected from 24 nursing mothers over 3 consecutive d were evaluated for sweetness, viscosity, and mouthcoat by a trained adult panel using magnitude estimation. Milks were perceived as sweet, thin, and low in mouthcoat. Hind milks were slightly more viscous and mouthcoating than fore milks. The higher fat content in hind milk was associated with higher estimation of physical viscosity. Milk fat content decreased as maternal age increased. Significant differences occurred between milks in lactose, fat, and protein contents. By use of a sweetness power function curve, the sweetness intensity of human milk was estimated as equivalent to a 2.12% sucrose solution.     

Comparative Cytotoxicity of Artemisinin and Cisplatin and Their Interactions with Chlorogenic Acids in MCF7 Breast Cancer Cells

In parts of Africa and Asia, self‐medication with a hot water infusion of Artemisia annua (Artemisia tea) is a common practice for a number of ailments including malaria and cancer. In our earlier work, such an extract showed better potency than artemisinin alone against both chloroquine‐sensitive and ‐resistant parasites. In this study, in vitro tests of the infusion in MCF7 cells showed high IC₅₀ values (>200 μM ). The combination of artemisinin and 3‐caffeoylquinic acid (3CA), two major components in the extract, was strongly antagonistic and gave a near total loss of cytotoxicity for artemisinin. We observed that the interaction of 3CAs with another cytotoxic compound, cisplatin, showed potentiation of activity by 2.5‐fold. The chelation of cellular iron by 3CA is hypothesized as a possible explanation for the loss of artemisinin activity.