Targeted Therapy in the Treatment of Pediatric Acute Lymphoblastic Leukemia—Therapy and Toxicity Mechanisms

Targeted therapy has revolutionized the treatment of poor-prognosis pediatric acute lymphoblastic leukemia (ALL) with specific genetic abnormalities. It is still being described as a new landmark therapeutic approach. The main purpose of the use of molecularly targeted drugs and immunotherapy in the treatment of ALL is to improve the treatment outcomes and reduce the doses of conventional chemotherapy, while maintaining the effectiveness of the therapy. Despite promising treatment results, there is limited clinical research on the effect of target cell therapy on the potential toxic events in children and adolescents. The recent development of highly specific molecular methods has led to an improvement in the identification of numerous unique expression profiles of acute lymphoblastic leukemia. The detection of specific genetic mutations determines patients’ risk groups, which allows for patient stratification and for an adjustment of the directed and personalized target therapies that are focused on particular molecular alteration. This review summarizes the knowledge concerning the toxicity of molecular-targeted drugs and immunotherapies applied in childhood ALL.     

Ruthenium Complexes with 2-Pyridin-2-yl-1H-benzimidazole as Potential Antimicrobial Agents: Correlation between Chemical Properties and Anti-Biofilm Effects

Antimicrobial resistance is a growing public health concern that requires urgent action. Biofilm-associated resistance to antimicrobials begins at the attachment phase and increases as the biofilms maturate. Hence, interrupting the initial binding process of bacteria to surfaces is essential to effectively prevent biofilm-associated problems. Herein, we have evaluated the antibacterial and anti-biofilm activities of three ruthenium complexes in different oxidation states with 2-pyridin-2-yl-1H-benzimidazole (L₁ = 2,2′-PyBIm): [(η⁶-p-cymene)Ru^IIClL₁]PF₆ (Ru(II) complex), mer-[Ru^IIICl₃(CH₃CN)L₁]·L₁·3H₂O (Ru(III) complex), (H₂L₁)₂[Ru^IIICl₄(CH₃CN)₂]₂[Ru^IVCl₄(CH₃CN)₂]·2Cl·6H₂O (Ru(III/IV) complex). The biological activity of the compounds was screened against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa strains. The results indicated that the anti-biofilm activity of the Ru complexes at concentration of 1 mM was better than that of the ligand alone against the P. aeruginosa PAO1. It means that ligand, in combination with ruthenium ion, shows a synergistic effect. The effect of the Ru complexes on cell surface properties was determined by the contact angle and zeta potential values. The electric and physical properties of the microbial surface are useful tools for the examined aggregation phenomenon and disruption of the adhesion. Considering that intermolecular interactions are important and largely define the functions of compounds, we examined interactions in the crystals of the Ru complexes using the Hirshfeld surface analysis.     

The application of macroalga Pithophora varia Wille enriched with microelements by biosorption as biological feed supplement for livestock

BACKGROUND: The paper discusses biosorption of microelements by a freshwater macroalga Pithophora varia Wille to produce biological feed supplement for livestock. The biomass was enriched with microelements recommended by feeding standards in a single and a multi‐metal system. RESULTS: The equilibrium of biosorption was described by using a Langmuir model and the following values of the maximum biosorption capacity in the single‐metal system were obtained: Zn(II), 61.1 mg g^?1; Co(II), 52.3 mg g^?1; Cu(II), 55.7 mg g^?1; and Mn(II), 38.3 mg g^?1. The average value of maximum biosorption capacity expressed in molar units for all the cations was equal 1.7 ± 0.2 meq g^?1, suggesting chemical rather than physical biosorption. It was also found that the mechanism of biosorption was due to cation exchange of alkali and alkaline earth metals with microelements. The biomass was also enriched with microelements in multi‐metal system. The total metal ion binding capacity in the multi‐metal system was two‐fold lower than in the single‐metal system and was 0.90 meq g^?1 in the preparation for laying hens and 0.95 meq g^?1 in the supplement for swine. CONCLUSION: Finally, the level of supplementation of livestock feed (with enriched single‐ and multi‐metal system macroalga), to cover 25% of total requirements for microelements, according to feeding standards, was provided. Copyright © 2008 Society of Chemical Industry     

Comparison of antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from humans and chicken carcasses in Poland

Campylobacter-associated gastroenteritis remains an important cause of morbidity worldwide, and some evidence suggests that poultry is an important source of this foodborne infection in humans. This study was conducted to analyze the prevalence and genetic background of resistance of 149 Campylobacter jejuni and 54 Campylobacter coli strains isolated from broiler chicken carcasses and from stool samples of infected children in Poland from 2003 through 2005. Nearly all isolates were susceptible to macrolides and aminoglycosides. The highest resistance in both human and chicken strains was observed for ciprofloxacin (more than 40%), followed by ampicillin (13 to 21%), and tetracycline (8 to 29%). Resistance to ampicillin and tetracycline rose significantly between 2003 and 2005. Slight differences in resistance between human and chicken isolates indicate that although chicken meat is not the only source of Campylobacter infection in our population, it can be involved in the transmission of drug-resistant Campylobacter strains to humans.     

Hedonic tests in different locations as predictors of apple juice consumption at home in elderly and young subjects

The objective of this study was to evaluate the predictive value of hedonic tests on apple juice carried out in a sensory laboratory, University common room and at home (post-consumption test) on ad libitum home consumption measured over 5 consecutive days. Thirty-five elderly volunteers (59–88 years old) and 33 young subjects (20–30 years old) assessed the degree of liking five apple juices varying in sweetness (0, 2, 4, 7 and 10% w/w sucrose added) on a nine-point hedonic scale. Both age groups judged similarly the juice with the lowest sugar concentrations, whereas the juice with 2% sugar added received lower scores from the elderly compared to the young participants. Those samples with higher sweetness (4, 7 and 10%) received higher scores from the elderly compared to the young people. The sample with no sugar added had higher mean score (all the subjects) in the home test (6.92±1.78) compared to both laboratory (5.51±2.15) and common room (5.92±2.08). No such differences were observed for the juices with the other sugar contents. In the elderly group, 1-day intake of apple juice remained on a similar level, regardless of sweetness liking, while among young adults the intake varied, and was highest for juices that were liked most (with 0 and 2% sucrose added). The results showed that hedonic ratings have a limited value as predictors of fruit juice consumption at home. The correlation between rated degree of liking and intake was low, especially for the elderly (r=0.39), indicating that factors other than pleasantness may affect intake. Among three test conditions, the lowest correlation of juice intake was obtained with laboratory test results (r=0.38), relatively higher—when hedonic tests were conducted in common room setting (r=0.49), or as the post-consumption test at home (r=0.73). Further research is required on sensory procedures relevant for the elderly, which together with some other nonsensory factors, would give a better prediction of consumption.     

Resource recovery from source separated domestic waste(water) streams; full scale results

A major fraction of nutrients emitted from households are originally present in only 1% of total wastewater volume. New sanitation concepts enable the recovery and reuse of these nutrients from feces and urine. Two possible sanitation concepts are presented, with varying degree of source separation leading to various recovery products. Separate vacuum collection and transport followed by anaerobic treatment of concentrated black water (BW) demonstrated on a scale of 32 houses preserve 7.6 g/N/p/d and 0.63 gP/p/d amounting to respectively 69 and 48% of the theoretically produced N and P in the household, and 95% of the retained P was shown to be recoverable via struvite precipitation. Reuse of the anaerobic sludge in agriculture can substantially increase the P recovery. Energy recovery in the form of biogas from anaerobic digestion of concentrated BW, fits well in new concepts of sustainable, zero energy buildings. Nutrient recovery from separately collected urine lowers the percentage of nutrient recovery in comparison with BW but can, on the other hand, often be implemented in existing sanitation concepts. Theoretically 11gN/p/d and 1.0 g P/p/d are produced with urine, of which 38-63 and 34-61% were recovered in practice on a scale of 8-160 inhabitants in Sweden. New sanitation concepts with resource recovery and reuse are being demonstrated worldwide and more and more experience is being gained.     

Perceptions of the health and convenience characteristics of fresh and dried fruits

Dried fruits consumption has not received much research attention, although these foods are associated with potential health benefits. Therefore, the aim of this study was to compare fresh fruits and dried fruits on measures of health, convenience and consumer feelings.An on-line questionnaire that measured consumers' perceptions of the health benefits and convenience characteristics and their feelings about the consumption of both fresh fruits (apples and plums) and dried fruits (e.g., raisins, dates, and apricots) was completed by 693 respondents (Polish, French, and Dutch).Generally, fresh fruits were perceived to be healthier and less convenient than dried fruits. Consumers also reported more positive feelings about the consumption of fresh fruits compared to dried fruits. The more that the respondents were willing to sacrifice for their health, the more positively they rated the health aspects of both fresh and dried fruits, as well as several perceptions of convenience of both fresh and dried fruits. Health preoccupation was unrelated to most of the convenience perceptions and was associated with lower scores on several perceptions of healthiness. Recommendations for the consumer-oriented development of dried fruits are also provided.     

Studies on the usefulness of Cucurbita maxima for the production of ready-to-eat dried vegetable snacks with a high carotenoid content

Recent research indicates a beneficial influence of a diet rich in β-carotene on human health. For that reason, experiments were undertaken to process winter squash with a high carotenoid content into ready-to-eat dried snacks. Sensory quality and dietary value of chips and crispy cubes made from different cultivars were investigated.The suitability of winter squash for drying purposes depended mainly on the dry matter content in the raw material. Although all the investigated cultivars can be used for producing chips, obtaining high quality porous cubes requires at least 15% of dry matter. ‘Justynka F1’ emerged as the most suitable cultivar for processing; both chips and cubes produced from its fruits were characterized by a high sensory quality and contained significant amounts of beta-carotene (225- 253 μg g-1). Also, the cultivar ‘Amazonka’ could be considered for the production of chips as it allowed us to obtain a product with a high beta-carotene content (200 μg g-1) and good sensory properties. The dried ready-to-eat crispy vegetable snacks made from the new cultivars of winter squash could be exploited as a novel attractive product with the attractive taste and colour to serve as a valuable source of carotenoids in human diet.